LOCUS       Exported                5245 bp ds-DNA     circular SYN 11-NOV-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 12, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     2589..2607
                     /label=TV-seq-L2
                     /note="color: black; sequence: agctatgaccatgattacg"
     misc_feature    2681..2714
                     /label=vLOXP
                     /note="color: #ff9900; direction: RIGHT"
     misc_feature    2715..3212
                     /label=hGH polyA
                     /note="color: #a6acb3"
     primer_bind     2715..2736
                     /label=loxp 5' EcoRI
                     /note="color: black; sequence: 
                     GCCGCCgaattccgatcatattcaataacccttaatataacttcgtataatgtatgcta
                     tacgaagttatagcgctgctcgagagatctacg"
     primer_bind     complement(3192..3247)
                     /label=hGHpA 3' MluI
                     /note="color: black; sequence: 
                     gccgccACGCGTggctggacgtaaactcctcttcagacctaaaggacagggaagggagc
                     agt"
     primer_bind     3213..3237
                     /label=PL752 construction1 foward
                     /note="color: black; sequence: 
                     ggcggcgaattcTCAATTTCTGAGAACTGTCATTCTCGGAAATTGAtaggtctgaagag
                     gagtttacgtcc"
     primer_bind     3239..3269
                     /label=PGK MluI 5'
                     /note="color: black; sequence: 
                     gccgccACGCGTaggatctcgacctcgaaattct"
     primer_bind     3249..3259
                     /label=PL752 construction2 reverse
                     /note="color: black; sequence: 
                     ggcggcggatccTCAATTTCCGAGAATGACAGTTCTCAGAAATTGAattaagggttccg
                     gatctcgacc"
     promoter        3264..3776
                     /label=mouse PGK promoter
                     /note="color: #c040ff; direction: RIGHT"
     primer_bind     complement(3529..3547)
                     /label=PL552 PGK promoter primer 3'
                     /note="color: black; sequence: AGCGGTGCTGTCCATCTGC"
     primer_bind     complement(3781..3808)
                     /label=PGK 3' hindIII
                     /note="color: black; sequence: 
                     GCCGCCaagcttcagctgctcgagatctagatgg"
     exon            3812..4411
                     /label=PUR
                     /note="color: #ff6600; direction: RIGHT"
     polyA_signal    4412..4967
                     /label=PolyA
                     /note="color: #008000"
     primer_bind     complement(4449..4475)
                     /label=PL752 construction1 rev
                     /note="color: black; sequence: 
                     ggcggcatatcatcgatgcatggggtcgtgcgc"
     primer_bind     4460..4482
                     /label=PL752 construction2 foward
                     /note="color: black; sequence: 
                     ggcggcatgcatcgatgatatcagatcc"
     primer_bind     4634..4651
                     /label=PL552 specific primer 5'
                     /note="color: black; sequence: TTAGATAAATGCCTGCTC"
     primer_bind     4868..4887
                     /label=vCre testing genotyping F
                     /note="color: black; sequence: acgagatcagcagcctctgt"
     primer_bind     complement(4945..5007)
                     /label=PL752 construction2 reverse
                     /note="color: black; sequence: 
                     ggcggcggatccTCAATTTCCGAGAATGACAGTTCTCAGAAATTGAattaagggttccg
                     gatctcgacc"
     misc_feature    4968..5001
                     /label=vLOXP
                     /note="color: #ff9900; direction: RIGHT"
     primer_bind     complement(5071..5090)
                     /label=TV-seq-R
                     /note="color: black; sequence: aaacgacggccagtgaattg"
     primer_bind     complement(5094..5113)
                     /label=vCre testing genotyping R
                     /note="color: black; sequence: gttttcccagtcacgacgtt"
ORIGIN
        1 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
       61 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      421 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2581 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     2641 aaaagctggt accgggcccc ccctcgaggt cgacgaattc tcaatttctg agaactgtca
     2701 ttctcggaaa ttgaagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc
     2761 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     2821 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     2881 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     2941 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     3001 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     3061 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     3121 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     3181 aggcgtgaac cactgctccc ttccctgtcc tttaggtctg aagaggagtt tacgtccagc
     3241 cacgcgtagg atctcgacct cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca
     3301 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     3361 gcctcgcaca cattccacat ccaccggtag gcgccaaccg actccgttct ttggtggccc
     3421 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     3481 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     3541 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     3601 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     3661 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     3721 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat
     3781 ccatctagat ctcgagcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct
     3841 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3901 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3961 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4021 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4081 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4141 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4201 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     4261 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccaca acctcccctt
     4321 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     4381 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     4441 cgaaaggagc gcacgacccc atgcatcgat gatatcagat ccccgggatg cagaaattga
     4501 tgatctatta aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt
     4561 aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg
     4621 gggtggggtg ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta
     4681 tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag
     4741 ctcattcctc ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc
     4801 tcttgattcc cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc
     4861 ctgaagaacg agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc
     4921 caagttctaa ttccatcaga agctggtcga gatccggaac ccttaattca atttctgaga
     4981 actgtcattc tcggaaattg aggatccact agttctagag cggccgccac cgcggtggag
     5041 ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     5101 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5161 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5221 gaatggcgaa tggaaattgt aagcg
//
