LOCUS       Exported               11275 bp ds-DNA     circular SYN 23-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   skyou
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 12, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     939..967
                     /label=Neo-bGHpA 5' MfeI
     CDS             949..1752
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1790..2014
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1992..2014)
                     /label=Neo-bGHPA 3' SpeI
     enhancer        2082..2461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2463..2740
                     /label=chicken beta-actin promoter
     primer_bind     3813..3850
                     /label=NucleoPlas EGFP 5'
     CDS             3829..4596
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKRPAATKKAGQAKKKK"
     primer_bind     complement(4526..4606)
                     /label=NucleoPlas EGFP 3'
     misc_feature    4546..4593
                     /label=nucleoplasmin
     misc_feature    4630..5218
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5101..5112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5250..5726
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_site      5901..5956
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(6317..6333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6341..6357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6365..6395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6410..6431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(7382..7400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7407..7423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7561..7863
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(9260..10120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10244..10832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11120..11141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11156..11186
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11194..11210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11218..11234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11255..11273
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggat gggatcggcc
      961 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1021 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1081 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     1141 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1201 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1261 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1321 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1381 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     1441 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     1501 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     1561 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1621 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1681 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1741 gagttcttct gaggggatca attctctaga gctcgctgat cagcctcgac tgtgccttct
     1801 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     1861 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     1921 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     1981 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2041 ggctcgacta gagcttgcgg aacccttaat ggtaccgtct cgacattgat tattgactag
     2101 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     2161 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2221 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     2281 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     2341 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     2401 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2461 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     2521 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     2581 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     2641 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     2701 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg gggagtcgct gcgacgctgc
     2761 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     2821 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
     2881 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
     2941 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     3001 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
     3061 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     3121 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     3181 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     3241 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     3301 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     3361 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     3421 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     3481 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     3541 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     3601 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     3661 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     3721 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     3781 attgtgctgt ctcatcattt tggcaaagaa ttcgtcgacg ccgccaccat ggtgagcaag
     3841 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3901 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3961 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4021 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     4081 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4141 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4201 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4261 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     4321 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     4381 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     4441 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     4501 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaagcg acctgccgcc
     4561 acaaagaagg ctggacaggc taagaagaag aaataatgaa cgcgtgaatt cgatatcaag
     4621 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4681 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4741 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4801 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4861 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4921 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4981 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5041 tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5101 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5161 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5221 cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc
     5281 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     5341 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     5401 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     5461 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     5521 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     5581 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     5641 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     5701 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccaggcgc gtgaattcac
     5761 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     5821 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     5881 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     5941 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     6001 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     6061 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     6121 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     6181 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6241 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     6301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6481 agcggatcga cagtactaag ctttactagg gacaggattg gtgacagaaa agccccatcc
     6541 ttaggcctcc tccttcctag tctcctgata ttgggtctaa cccccacctc ctgttaggca
     6601 gattccttat ctggtgacac acccccattt cctggagcca tctctctcct tgccagaacc
     6661 tctaaggttt gcttacgatg gagccagaga ggatcctggg agggagagct tggcaggggg
     6721 tgggagggaa gggggggatg cgtgacctgc ccggttctca gtggccaccc tgcgctaccc
     6781 tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc
     6841 tgcagcttcc ttacacttcc caagaggaga agcagtttgg aaaaacaaaa tcagaataag
     6901 ttggtcctga gttctaactt tggctcttca cctttctagt ccccaattta tattgttcct
     6961 ccgtgcgtca gttttacctg tgagataagg ccagtagcca gccccgtcct ggcagggctg
     7021 tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt
     7081 gttcaccagg tcgtggccgc ctctactccc tttctctttc tccatccttc tttccttaaa
     7141 gagtccccag tgctatctgg gacatattcc tccgcccaga gcagggtccc gcttccctaa
     7201 ggccctgctc tgggcttctg ggtttgagtc cttggcaagc ccaggagagg cgctcaggct
     7261 tccctgtccc ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc ccttccctac
     7321 aggggttcct ggctctgctc taagggcaag ggcgaattcg cggccgctaa attcaattcg
     7381 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     7441 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7501 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct atacgtacgg
     7561 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     7621 agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag tgcacgtctg
     7681 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     7741 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     7801 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     7861 taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt cacgtagaaa
     7921 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
     7981 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
     8041 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
     8101 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga
     8161 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     8221 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     8281 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     8341 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     8401 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     8461 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     8521 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     8581 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     8641 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     8701 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     8761 ctcgtcgtga cccatgcatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     8821 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     8881 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     8941 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     9001 gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     9061 gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     9121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagatta tcaaaaagga
     9181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9841 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10141 attgaagcat ttatcagggt tattgtctca tgaccaaaat cccttaacgt gagttttcgt
    10201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    11041 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    11101 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    11161 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    11221 gaaacagcta tgaccatgat tacgccaagc tcagaattaa ccctcactaa agggt
//
