LOCUS       Exported                7739 bp ds-DNA     circular SYN 31-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 12, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        386..661
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     primer_bind     1599..1619
                     /label=CAG-F
                     /note="CAG-F"
                     /note="color: black; sequence: GGCTTCTGGCGTGTGACCGGC"
     primer_bind     1704..1742
                     /label=h003 sp F
                     /note="color: black; sequence: 
                     CATCATTTTGGCAAAGGCCACCATGCTGCCTGGGCGGCT"
     primer_bind     1705..1747
                     /label=Hg-016-CSTN2-F3
                     /note="color: black; sequence: 
                     ATCATTTTGGCAAAGGCCACCATGCTGCCTGGGCGGCTGTGCT"
     misc_feature    1705..1719
                     /label=recombination fragment front
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1720..1728
                     /label=KOZAK fragment
                     /note="color: #ff9900; direction: RIGHT"
     CDS             1726..4683
                     /codon_start=1
                     /label=CSTN2
                     /note="color: #993366"
                     /translation="MLPGRLCWVPLLLALGVGSGSGGGGDSRQRRLLAAKVNKHKPWIE
                     TSYHGVITENNDTVILDPPLVALDKDAPVPFAGEICAFKIHGQELPFEAVVLNKTSGEG
                     RLRAKSPIDCELQKEYTFIIQAYDCGAGPHETAWKKSHKAVVHIQVKDVNEFAPTFKEP
                     AYKAVVTEGKIYDSILQVEAIDEDCSPQYSQICNYEIVTTDVPFAIDRNGNIRNTEKLS
                     YDKQHQYEILVTAYDCGQKPAAQDTLVQVDVKPVCKPGWQDWTKRIEYQPGSGSMPLFP
                     SIHLETCDGAVSSLQIVTELQTNYIGKGCDRETYSEKSLQKLCGASSGIIDLLPSPSAA
                     TNWTAGLLVDSSEMIFKFDGRQGAKVPDGIVPKNLTDQFTITMWMKHGPSPGVRAEKET
                     ILCNSDKTEMNRHHYALYVHNCRLVFLLRKDFDQADTFRPAEFHWKLDQICDKEWHYYV
                     INVEFPVVTLYMDGATYEPYLVTNDWPIHPSHIAMQLTVGACWQGGEVTKPQFAQFFHG
                     SLASLTIRPGKMESQKVISCLQACKEGLDINSLESLGQGIKYHFNPSQSILVMEGDDIG
                     NINRALQKVSYINSRQFPTAGVRRLKVSSKVQCFGEDVCISIPEVDAYVMVLQAIEPRI
                     TLRGTDHFWRPAAQFESARGVTLFPDIKIVSTFAKTEAPGDVKTTDPKSEVLEEMLHNL
                     DFCDILVIGGDLDPRQECLELNHSELHQRHLDATNSTAGYSIYGVGSMSRYEQVLHHIR
                     YRNWRPASLEARRFRIKCSELNGRYTSNEFNLEVSILHEDQVSDKEHVNHLIVQPPFLQ
                     SVHHPESRSSIQHSSVVPSIATVVIIISVCMLVFVVAMGVYRVRIAHQHFIQETEAAKE
                     SEMDWDDSALTITVNPMEKHEGPGHGEDETEGEEEEEAEEEMSSSSGSDDSEEEEEEEG
                     MGRGRHGQNGARQAQLEWDDSTLPYYPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    1726..1785
                     /label=signal peptides
                     /note="color: #a6acb3"
     primer_bind     complement(1726..1784)
                     /label=h004 sp M
                     /note="color: black; sequence: 
                     CCGCTCCCCACGCCCAGCGCCAGCAGGAGCGGCACCCAGCACAGCCGCCCAGGCAGCAT
                     "
     misc_feature    1726..1728
                     /label=ATG
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     1729..1747
                     /label=Hg-012-CSTN2-F1R
                     /note="color: black; sequence: 
                     GGATCCTATCCATATGACGTTCCAGATTACGCTCTGCCTGGGCGGCTGTGCT"
     primer_bind     complement(1766..1785)
                     /label=h005 sp R
                     /note="color: black; sequence: 
                     AGGGATAGCCAGCGTAATCTGGAACATCGTATGGGTAGCCGCTCCCCACGCCCAGCG"
     primer_bind     1786..1802
                     /label=h006 3XHA-C F
                     /note="color: black; sequence: 
                     GATCCTATCCATATGACGTTCCAGATTACGCTAGCGGCGGTGGCGGGGA"
     primer_bind     1939..1958
                     /label=AgeI-clsMid-F
                     /note="color: black; sequence: GCCGCaccggttccttttgcagg"
     primer_bind     2654..2673
                     /label=F1
                     /note="color: black; sequence: GTGACCGGGAGACCTACTCT"
     primer_bind     2752..2771
                     /label=Hg-014-CSTN2-F2/F4
                     /note="color: black; sequence: TGGACTGCAGGACTGCTGGT"
     primer_bind     complement(2752..2771)
                     /label=Hg-013-CSTN2-R1/R3
                     /note="color: black; sequence: ACCAGCAGTCCTGCAGTCCA"
     primer_bind     complement(2753..2811)
                     /label=h009 CLSTN2-1 R
                     /note="color: black; sequence: 
                     ctgcctgccgtcaaacttgaagatcatctcactgctgtccaccagcagtcctgcagtcc
                     "
     primer_bind     2793..2823
                     /label=h010 CLSTN2-2 F
                     /note="color: black; sequence: 
                     caagtttgacggcaggcagggtgccaaagtc"
     primer_bind     2805..2823
                     /label=h002 CSTN2 F
                     /note="color: black; sequence: CAGGCAGGGTGCCAAAGTC"
     primer_bind     complement(2805..2822)
                     /label=h001 CSTN2 R
                     /note="color: black; sequence: ACTTTGGCACCCTGCCTG"
     primer_bind     complement(2945..2964)
                     /label=R1
                     /note="color: black; sequence: GTGATGCCGGTTCATTTCGG"
     primer_bind     complement(4202..4218)
                     /label=NotI-cls-extra-R
                     /note="color: black; sequence: 
                     ATTATTgcggccgcctggaagttctgttccaggggcccgcttgggaccactgaac"
     primer_bind     complement(4202..4218)
                     /label=XbaI-Cls-extra-R
                     /note="color: black; sequence: 
                     GCCGCtctagagcttgggaccactgaac"
     primer_bind     complement(4572..4625)
                     /label=Hg-017-CSTN2-R4L
                     /note="color: black; sequence: 
                     GGATAGCCAGCGTAATCTGGAACATCGTATGGGTAGTAGGGGAGGGTGGAGTCA"
     primer_bind     complement(4574..4592)
                     /label=Hg-015-CSTN2-R2
                     /note="color: black; sequence: 
                     GCACCTGAGGAGTGCCTAGTAGGGGAGGGTGGAGT"
     CDS             4591..4680
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     4591..4626
                     /label=Hg-011-CSTN2-F1L
                     /note="color: black; sequence: 
                     ATCATTTTGGCAAAGGCCACCATGTACCCATACGATGTTCCAGATTACGCTGGCTATCC
                     C"
     primer_bind     4610..4663
                     /label=Hg-009-Mrear
                     /note="color: black; sequence: 
                     ATTACGCTGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATG"
     primer_bind     complement(4610..4663)
                     /label=Hg-002-Mfront
                     /note="color: black; sequence: 
                     CATATGGATAGGATCCTGCATAGTCCGGGACGTCATAGGGATAGCCAGCGTAAT"
     primer_bind     complement(4642..4698)
                     /label=Hg-018-CSTN2-R4R
                     /note="color: black; sequence: 
                     GCACCTGAGGAGTGCCTAAGCGTAATCTGGAACGTCATATGGATAGGATCCTGCATA"
     misc_feature    4684..4698
                     /label=recombination fragment rear
                     /note="color: #00ff00; direction: RIGHT"
     primer_bind     complement(4723..4742)
                     /label=CAG-R
                     /note="CAG-R"
                     /note="color: black; sequence: CAGGGCATTGGCCACACCAG"
     polyA_site      4828..4883
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5242..5258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5266..5282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5290..5320)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5290 .. 5296 / #ffffff / -10
                        2: 5297 .. 5314 / #ffffff
                        3: 5315 .. 5320 / #ffffff / -35"
     protein_bind    5335..5356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        5415..5610
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5461..5596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     polyA_signal    5616..5750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5989..6577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6748..7608)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6748 .. 7539 / #ccffcc
                        2: 7540 .. 7608 / #ccffcc / signal sequence
                     Cleavage site after base 7539"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7609..7713)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaagg ccaccatgct gcctgggcgg
     1741 ctgtgctggg tgccgctcct gctggcgctg ggcgtgggga gcggcagcgg cggtggcggg
     1801 gacagccggc agcgccgcct cctcgcggct aaagtcaata agcacaagcc atggatcgag
     1861 acttcatatc atggagtcat aactgagaac aatgacacag tcattttgga cccaccactg
     1921 gtagccctgg ataaagatgc accggttcct tttgcagggg aaatctgtgc gttcaagatc
     1981 catggccagg agctgccctt tgaggctgtg gtgctcaaca agacatcagg agagggccgg
     2041 ctccgtgcca agagccccat tgactgtgag ttgcagaagg agtacacatt catcatccag
     2101 gcctatgact gtggtgctgg gccccacgag acagcctgga aaaagtcaca caaggccgtg
     2161 gtccatatac aggtgaagga tgtcaacgag tttgctccca ccttcaaaga gccagcctac
     2221 aaggctgttg tgacggaggg caagatctat gacagcattc tgcaggtgga ggccattgac
     2281 gaggactgct ccccacagta cagccagatc tgcaactatg aaatcgtcac cacagatgtg
     2341 ccttttgcca tcgacagaaa tggcaacatc aggaacactg agaagctgag ctatgacaaa
     2401 caacaccagt atgagatcct ggtgaccgcc tacgactgtg gacagaagcc cgctgctcag
     2461 gacaccctgg tgcaggtgga tgtgaagcca gtttgcaagc ctggctggca agactggacc
     2521 aagaggattg agtaccagcc tggctccggg agcatgcccc tgttccccag catccacctg
     2581 gagacgtgcg atggagccgt gtcttccctc cagatcgtca cagagctgca gactaattac
     2641 attgggaagg gttgtgaccg ggagacctac tctgagaaat cccttcaaaa gttatgtgga
     2701 gcctcctctg gcatcattga cctcttgcca tcccctagcg ctgccaccaa ctggactgca
     2761 ggactgctgg tggacagcag tgagatgatc ttcaagtttg acggcaggca gggtgccaaa
     2821 gtccccgatg ggattgtgcc caagaacctg accgatcagt tcaccatcac catgtggatg
     2881 aaacacggcc ccagccctgg tgtgagagcc gagaaggaaa ccatcctctg caactcagac
     2941 aaaaccgaaa tgaaccggca tcactatgcc ctgtatgtgc acaactgccg cctcgtcttt
     3001 ctcttgcgga aggacttcga ccaggctgac acctttcgcc ccgcggagtt ccactggaag
     3061 ctggatcaga tttgtgacaa agagtggcac tactatgtca tcaatgtgga gtttcctgtg
     3121 gtaaccttat acatggatgg agcaacatat gaaccatacc tggtgaccaa cgactggccc
     3181 attcatccat ctcacatagc catgcaactc acagtcggcg cttgttggca aggaggagaa
     3241 gtcaccaaac cacagtttgc tcagttcttt catggaagcc tggccagtct caccatccgc
     3301 cctggcaaaa tggaaagcca gaaggtgatc tcctgcctgc aggcctgcaa ggaagggctg
     3361 gacattaatt ccttggaaag ccttggccaa ggaataaagt atcacttcaa cccctcgcag
     3421 tccatcctgg tgatggaagg tgacgacatt gggaacatta accgtgctct ccagaaagtc
     3481 tcctacatca actccaggca gttcccaacg gcgggtgtgc ggcgcctcaa agtatcctcc
     3541 aaagtccagt gctttgggga agacgtatgc atcagtatcc ctgaggtaga tgcctatgtg
     3601 atggtcctcc aggccatcga gccccggatc accctccggg gcacagacca cttctggaga
     3661 cctgctgccc agtttgaaag tgccagggga gtgaccctct tccctgatat caagattgtg
     3721 agcaccttcg ccaaaaccga agcccccggg gacgtgaaaa ccacagaccc caaatcagaa
     3781 gtcttagagg aaatgcttca taacttagat ttctgtgaca ttttggtgat cggaggggac
     3841 ttggacccaa ggcaggagtg cttggagctc aaccacagtg agctccacca acgacacctg
     3901 gatgccacta attctactgc aggctactcc atctacggtg tgggctccat gagccgctat
     3961 gagcaggtgc tacatcacat ccgctaccgc aactggcgtc cggcttccct tgaggcccgg
     4021 cgtttccgga ttaagtgctc agaactcaat gggcgctaca ctagcaatga gttcaacttg
     4081 gaggtcagca tccttcatga agaccaagtc tcagataagg agcatgtcaa tcatctgatt
     4141 gtgcagcctc ccttcctcca gtctgtccat catcctgagt cccggagtag catccagcac
     4201 agttcagtgg tcccaagcat tgccacagtg gtcatcatca tctccgtgtg catgcttgtg
     4261 tttgtcgtgg ccatgggtgt gtaccgggtc cggatcgccc accagcactt catccaggag
     4321 actgaggctg ccaaggaatc tgagatggac tgggacgatt ctgcgctgac tatcacagtc
     4381 aaccccatgg agaaacatga aggaccaggg catggggaag atgagactga gggagaagag
     4441 gaggaagaag ccgaggaaga aatgagctcc agcagtggct ctgacgacag cgaagaggag
     4501 gaggaggagg aagggatggg cagaggcaga catgggcaga atggagccag gcaagcccag
     4561 ctggagtggg atgactccac cctcccctac tacccatacg atgttccaga ttacgctggc
     4621 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     4681 taggcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     4741 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     4801 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     4861 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4921 atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa
     4981 caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     5041 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     5101 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     5161 tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct cgacctgcag
     5221 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5281 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5341 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5401 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5461 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5521 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5581 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     5641 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5701 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     5761 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6181 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6421 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6721 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6781 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6841 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6901 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6961 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7021 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7081 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7141 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7201 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7261 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7321 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7381 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7441 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7501 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7561 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7621 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7681 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
