LOCUS       CN2237_rAAV-eHGT_452h-minBglobin-SYFP2-WPRE3-BGHpA        4823 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...f6p-azzxtw2
VERSION     urn.local...f6p-azzxtw2
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2237_rAAV-eHGT_452h-minBglobin-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN2237_rAAV-eHGT_452h-minBglobin-SYFP2-WPRE3-BGHpA" 0 4823 
COMMENT       (CDBMol 0 0 1 1 1 0 0 10 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4823 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2762 0 3259 0 4505 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 9 41 0 52 0 315 0 591 0 746 
COMMENT            0 1842 0 2172 0 2183 0 2639 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 802 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1584 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 447 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 840 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2225 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2174 0 2185 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2172 0 2183 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3012 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 1563 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 8 848 1 881 1 1202 1 
COMMENT             1385 0 1535 1 2688 1 2885 1 3627 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 53 38 0 143 1 147 0 555 0 
COMMENT             1060 0 1101 0 1168 0 1207 0 1302 0 1345 0 1365 1 1518 0 1521 0 1574 
COMMENT             0 1576 1 1580 0 1769 1 1782 0 1795 0 2070 1 2079 1 2083 0 2188 1 
COMMENT             2238 1 2264 1 2277 0 2314 1 2328 1 2331 1 2359 0 2386 0 2791 0 2817 
COMMENT             0 2827 0 2866 0 3040 1 3087 0 3186 1 3295 1 3372 1 3416 0 3537 1 
COMMENT             3583 0 3774 1 3865 1 4227 0 4236 1 4371 0 4481 1 4602 1 4621 1 4748 
COMMENT             1 4776 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 2745 0 3329 0 3702 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 4 995 0 1238 0 3265 0 
COMMENT             4277 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 825 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 829 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 29 12 0 320 0 449 0 531 0 
COMMENT             745 0 820 0 862 0 895 0 967 0 979 0 1000 0 1216 0 1264 0 1375 0 
COMMENT             1449 0 1462 0 1549 0 2216 0 2445 0 2702 0 2880 0 2899 0 3578 0 3641 
COMMENT             0 3741 0 4262 0 4519 0 4565 0 4655 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 12 797 1 810 0 1336 1 
COMMENT             1535 0 3292 0 3296 1 3613 0 4076 1 4077 0 4173 1 4175 0 4261 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1569 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 3756 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 678 0 1498 0 2072 0 3567 
COMMENT             0 3789 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 663 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2232 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 206 1 2052 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 211 1 2047 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 20 21 0 518 1 987 1 1093 
COMMENT             0 1384 0 1410 1 1413 1 1666 1 1784 1 2203 1 2225 0 2362 0 2886 1 
COMMENT             3497 1 3888 0 4191 1 4397 1 4400 1 4490 0 4822 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 12 133 0 784 0 890 0 1142 
COMMENT             0 1412 0 1886 0 2094 1 2517 1 2549 0 3474 1 3761 1 3885 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 13 916 0 928 0 976 0 
COMMENT             1057 0 1168 0 1258 0 1336 0 1378 0 1429 0 1582 0 1782 0 2505 0 4319 
COMMENT             1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 13 916 0 928 0 976 0 
COMMENT             1057 0 1168 0 1258 0 1336 0 1378 0 1429 0 1582 0 1782 0 2505 0 4320 
COMMENT             1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 1667 1 3094 0 4621 0 
COMMENT             0 "") #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 10 131 0 345 0 664 0 821 0 
COMMENT             1867 0 2095 0 2379 0 3738 0 4073 0 4326 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 3 808 0 1848 0 2059 0 0 
COMMENT             "") #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 1463 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 3891 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 824 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1286 0 1526 0 3862 0 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 5 374 1 3327 0 4195 0 
COMMENT             4436 1 4734 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2169 0 2229 0 3009 
COMMENT             0 3391 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 3865 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 14 542 0 943 1 1217 0 
COMMENT             1232 0 1331 0 1973 0 2228 1 2264 0 2780 1 2870 0 3233 1 3478 0 3673 
COMMENT             1 4725 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 13 23 1 98 0 226 1 1436 
COMMENT             1 1454 1 1968 1 2132 1 2207 0 2783 0 3444 0 3961 1 4127 1 4536 1 0 
COMMENT             "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 2 872 0 1723 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 2 751 1 4515 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 970 1 1067 0 1391 0 0 
COMMENT             "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 4 2894 0 2936 1 3089 1 
COMMENT             3865 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 2894 0 2936 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 1 622 1 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 13 24 1 97 0 227 1 1437 
COMMENT             1 1455 1 1969 1 2133 1 2206 0 2782 0 3443 0 3962 1 4128 1 4537 1 0 
COMMENT             "") #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 2986 0 3091 0 4099 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2079 1 2388 0 3089 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 2 3697 0 3871 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1551 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 11 1453 1 1639 0 2621 0 
COMMENT             3286 0 3456 1 3725 0 3768 0 3886 0 4292 0 4404 1 4417 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1569 0 2124 
COMMENT             0 2209 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1022 1 2955 1 3262 0 
COMMENT             4646 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 815 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 8 222 1 713 0 882 1 1248 
COMMENT             1 2862 1 3485 0 3772 0 3953 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 22 381 0 805 0 811 0 984 0 
COMMENT             1344 0 1492 0 1530 0 1851 0 2062 0 3251 0 3287 0 3304 0 3562 0 3608 
COMMENT             0 3626 0 3967 0 4072 0 4084 0 4162 0 4170 0 4181 0 4256 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 12 279 0 887 0 1012 0 1034 
COMMENT             0 1124 0 1199 0 1253 0 1750 0 1917 0 4660 0 4673 0 4794 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 22 17 0 102 0 153 0 1170 0 
COMMENT             1488 0 1571 0 1576 0 1582 0 2126 0 2211 0 2304 0 2328 0 2348 0 2724 
COMMENT             0 2835 0 2938 0 2940 0 3040 0 3372 0 3865 0 4195 0 4776 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 40 12 0 15 0 40 0 146 0 
COMMENT             149 0 534 0 1003 0 1084 0 1368 0 1375 0 1426 0 1429 0 1523 0 1576 0 
COMMENT             1579 0 1582 0 1682 0 1784 0 1800 0 2082 0 2085 0 2191 0 2216 0 2219 
COMMENT             0 2317 0 2331 0 2353 0 2793 0 2902 0 3189 0 3418 0 3513 0 3540 0 
COMMENT             3879 0 4207 0 4413 0 4416 0 4481 0 4624 0 4779 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 5 636 0 733 0 1055 0 3512 
COMMENT             0 3532 1 0 "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 37 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 420 0 559 0 822 0 965 0 998 0 1046 0 1060 0 1370 
COMMENT             0 1377 0 1571 0 1786 0 1795 0 1875 0 2052 0 2126 0 2130 0 2134 0 
COMMENT             2203 0 2207 0 2211 0 2221 0 2372 0 2386 0 2429 0 2805 0 3818 0 4209 
COMMENT             0 4769 0 4806 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 10 949 1 1213 0 1228 0 1357 
COMMENT             0 1408 0 1780 0 1969 0 2545 0 3278 0 3679 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 22 382 0 806 0 812 0 985 0 
COMMENT             1345 0 1493 0 1531 0 1852 0 2063 0 3252 0 3288 0 3305 0 3563 0 3609 
COMMENT             0 3627 0 3968 0 4073 0 4085 0 4163 0 4171 0 4182 0 4257 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 6 490 0 1270 0 1552 0 2300 
COMMENT             0 2773 0 3449 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 18 376 0 419 0 841 0 1075 
COMMENT             0 1105 0 1495 0 1540 0 1700 0 2055 0 2903 0 2987 0 3092 0 3485 0 
COMMENT             3521 0 3599 0 3609 0 4100 0 4820 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 89 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 213 0 248 0 255 0 284 0 320 0 395 0 449 0 531 0 548 0 608 0 
COMMENT             668 0 745 0 751 0 775 0 820 0 862 0 895 0 915 0 967 0 979 0 1000 0 
COMMENT             1015 0 1038 0 1116 0 1216 0 1264 0 1284 0 1331 0 1375 0 1416 0 1449 
COMMENT             0 1462 0 1549 0 1579 0 1721 0 1811 0 1816 0 1841 0 1877 0 2082 0 
COMMENT             2110 0 2146 0 2177 0 2191 0 2216 0 2431 0 2445 0 2459 0 2540 0 2644 
COMMENT             0 2682 0 2702 0 2803 0 2807 0 2847 0 2880 0 2899 0 2953 0 3540 0 
COMMENT             3578 0 3641 0 3651 0 3741 0 3807 0 3816 0 3820 0 3846 0 3887 0 3899 
COMMENT             0 4262 0 4291 0 4334 0 4345 0 4410 0 4489 0 4514 0 4519 0 4565 0 
COMMENT             4655 0 4753 0 4779 0 4797 0 4808 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 15 32 0 84 0 235 0 299 0 
COMMENT             1445 0 1463 0 1977 0 2141 0 2193 0 2769 0 3004 0 3430 0 3970 0 4136 
COMMENT             0 4545 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 22 380 0 804 0 810 0 983 0 
COMMENT             1343 0 1491 0 1529 0 1850 0 2061 0 3250 0 3286 0 3303 0 3561 0 3607 
COMMENT             0 3625 0 3966 0 4071 0 4083 0 4161 0 4169 0 4180 0 4255 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3353 0 4045 0 4064 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 1577 0 2080 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 2 573 0 3132 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 1090 1 1334 1 1784 0 
COMMENT             3789 0 4617 0 4763 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 7 995 0 1238 0 1286 0 
COMMENT             1526 0 3265 0 3862 0 4277 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 869 0 1523 0 2146 0 2171 0 2172 0 2182 0 2183 0 2857 0 2892 0 
COMMENT             3393 0 3744 0 4440 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 2 320 0 745 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 2951 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1592 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 15 317 0 382 0 816 0 892 
COMMENT             0 1186 0 1213 0 1228 0 1357 0 1564 0 1589 0 1844 0 1855 0 2498 0 
COMMENT             3278 0 4722 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 18 375 0 418 0 840 0 1074 0 
COMMENT             1104 0 1494 0 1539 0 1699 0 2054 0 2902 0 2986 0 3091 0 3484 0 3520 
COMMENT             0 3598 0 3608 0 4099 0 4819 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 8 562 0 1067 0 1175 0 
COMMENT             1802 0 2324 1 2393 0 2824 0 2834 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 11 681 0 1017 0 1418 0 
COMMENT              1501 0 2075 0 2541 0 2954 0 3570 0 3792 0 3809 0 3888 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 40 10 0 13 0 38 0 144 0 
COMMENT              147 0 532 0 1001 0 1082 0 1366 0 1373 0 1424 0 1427 0 1521 0 1574 
COMMENT              0 1577 0 1580 0 1680 0 1782 0 1798 0 2080 0 2083 0 2189 0 2214 0 
COMMENT              2217 0 2315 0 2329 0 2351 0 2791 0 2900 0 3187 0 3416 0 3511 0 
COMMENT              3538 0 3877 0 4205 0 4411 0 4414 0 4479 0 4622 0 4777 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 8 209 1 720 0 869 1 1235 
COMMENT              1 2849 1 3492 0 3779 0 3960 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3708 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 8 144 1 144 0 913 0 1577 
COMMENT              1 1577 0 2080 0 2080 1 3538 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 24 37 0 82 0 118 0 146 0 
COMMENT              248 0 915 0 1015 0 1331 0 1416 0 1579 0 2082 0 2110 0 2146 0 2191 
COMMENT              0 2540 0 2682 0 2953 0 3540 0 3807 0 3887 0 4345 0 4779 0 4797 0 
COMMENT              4808 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "HgaI" "gacgc" 11 16 6 46 1 2177 0 2841 0 
COMMENT              3399 0 4129 1 4707 1 0 "") 
COMMENT        #108=(CRSite (CStringList) "HhaI" "gcgc" 4 0 32 17 0 19 0 102 0 104 0 
COMMENT              826 0 1131 0 1172 0 1488 0 1571 0 1573 0 2126 0 2128 0 2211 0 2213 
COMMENT              0 2231 0 2306 0 2319 0 2328 0 2350 0 2376 0 2384 0 2837 0 2940 0 
COMMENT              3040 0 3372 0 3709 0 3802 0 4195 0 4304 0 4478 0 4578 0 4645 0 0 
COMMENT              "") 
COMMENT        #109=(CRSite (CStringList) "HinfI" "gantc" 2 0 5 734 0 2580 0 2602 0 
COMMENT              3932 0 4449 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "HinP1I" "gcgc" 2 0 32 15 0 17 0 100 0 102 0 
COMMENT              824 0 1129 0 1170 0 1486 0 1569 0 1571 0 2124 0 2126 0 2209 0 2211 
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COMMENT                              "BpmI: 3 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                                             " N3: 4610 (on complementary strand)" 
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COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
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COMMENT                                             " N4: 4443 (on complementary strand)" 
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COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
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COMMENT                             "GCATCNNNNNNNNNN" "CGTAGNNNNNNNNNN") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    748..800
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    841..1560
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1595..1840
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1854..2057
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2086..2226
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2318..2624
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3143..4000
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4151..4818
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    156..741
                     /vntifkey="21"
                     /label=eHGT_452h
BASE COUNT     1132 a      1306 c      1235 g      1150 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaggga ggaactaaag tccattcata 
      181 tgtccatatt ctcaatttgg gaaaaaatgt ggctttgtct tcatcctctt ttcctgagtg 
      241 tataaggcca gaagccaaag gcaagagcag gacattccag gagcctttat gattgctcta 
      301 agtcacatct gctctcgagc tcccattcca ctgtgaaaaa tacctagtct tgaactttgg 
      361 agttccaaat tccacatgat cgacttcctc aaggcttttc acttgacgaa caaattgcat 
      421 gccaagaaat cgtcaaaatt ctatcaagct taattatgca atttctgtct ataaatgaca 
      481 tacctcctgt accctatgac acccacctat gcactaataa gatgtgacag ctgcatcacc 
      541 aacaaagcct cccccgcctg ccattctctt cggaggtgag cacctcctgc tcgggaggaa 
      601 ctgacagccc ctttcagaaa tgtgaaggca gtgaagtccc aaaccagaaa cagtgtgacc 
      661 acctaggctc acccaaggac cgtcctgtgc ttctgggcaa ccaatggatg tagggggtta 
      721 ggaggcagtg gggattcgtc agagctcggg ctgggcataa aagtcagggc agagccatct 
      781 attgcttaca tttgcttctg ggatccagat ctttcgaagc tagcgctacc ggtcgccacc 
      841 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 
      901 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 
      961 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc 
     1021 ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag 
     1081 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 
     1141 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 
     1201 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 
     1261 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac 
     1321 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc 
     1381 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 
     1441 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc 
     1501 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 
     1561 gtcgacggcg cgccgcggcc gcgaattcga tatcataatc aacctctgga ttacaaaatt 
     1621 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     1681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     1741 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     1801 tggacagggg ctcggctgtt gggcactgac aattccgtgg ctcgagagat cttcgactgt 
     1861 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 
     1921 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 
     1981 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 
     2041 agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga 
     2101 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg 
     2161 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc 
     2221 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
     2281 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2341 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     2401 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     2461 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 
     2521 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     2581 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     2641 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     2701 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg 
     2761 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 
     2821 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 
     2881 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 
     2941 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 
     3001 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     3061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     3121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     3181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     3241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     3301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     3361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     3421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     3481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     3541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     3601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     3661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     3721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     3781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     3841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     3901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     3961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     4021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     4081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     4141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     4201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     4261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     4321 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     4381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     4441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     4501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     4561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     4621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     4681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     4741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     4801 ttgctggcct tttgctcaca tgt 
//
