LOCUS       CN2747\pAAV-eHGT_784m-minBG-SYFP2-WPRE3-BGHpA\(JT)        4727 bp    DNA     circular     13-OCT-2022
SOURCE      
  ORGANISM  
COMMENT     rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
            
            clones 2,3,4 all good
            clone 1 low yield 
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14611375|
COMMENT     VNTDBDATE|-14611375|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2747 pAAV-eHGT_784m-minBG-SYFP2-WPRE3-BGHpA (JT)|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    1990..2130
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2222..2528
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3047..3904
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4055..4722
                     /vntifkey="33"
                     /label=pUC\ori
     primer          complement(1872..1891)
                     /vntifkey="27"
                     /label=BGH-R
     misc_feature    1758..1961
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1499..1744
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1499..1658
                     /vntifkey="21"
                     /label=gamma
     misc_feature    1664..1744
                     /vntifkey="21"
                     /label=alpha
     misc_feature    complement(1612..1631)
                     /vntifkey="21"
                     /label=WPRE3-R\(JT)
     misc_feature    652..704
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    661..666
                     /vntifkey="21"
                     /label=TATA\box
     misc_feature    693..693
                     /vntifkey="21"
                     /label=transcriptional\start
     misc_feature    745..1464
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    complement(667..687)
                     /vntifkey="21"
                     /label=minBG-PCR-R
     misc_feature    156..645
                     /vntifkey="21"
                     /label=eHGT_784m
BASE COUNT     1088 a      1254 c      1254 g      1131 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaggaa ctggacacac aaagcctttc 
      181 tcgttttttc ttcctccctg tgtttcggtc caggggtcct aagtgtgtag ctcccgggga 
      241 gatgtgggac tgttctcacc cagaaagagg aactagaacc acaagagcct aaccccccac 
      301 ggtgggaaga cactctgtgg catcaccggg aggggaggcc aggtgtgggc aactgcagct 
      361 atttctaatc caccagagga aagagagctt tgactgcttg aaaagcaggc tgattgtcag 
      421 gggcggaacc cagctcagag ctgtcaggga agctcctggc agggggtgga gacagggggc 
      481 ttggactgac aggcctgtga ttttgaaaga aatgtcttta agagagtgac ttaaagtagg 
      541 agagaaatat tattattagg taatgcctgt caggttatat ttttttttga tgtgttcttg 
      601 gtatttcata atcattttgc ctttagatgg taagcccttc ctggtgagct cgggctgggc 
      661 ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg 
      721 aagctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg 
      781 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 
      841 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc 
      901 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt gcagtgcttc 
      961 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 
     1021 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 
     1081 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 
     1141 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 
     1201 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc 
     1261 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 
     1321 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc 
     1381 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 
     1441 ggcatggacg agctgtacaa gtaagtcgac ggcgcgccgc ggccgcgaat tcgatatcat 
     1501 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 
     1561 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 
     1621 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc 
     1681 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 
     1741 gtggctcgag agatcttcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 
     1801 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 
     1861 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 
     1921 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gagatctcac gtgcggaccg 
     1981 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc 
     2041 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag 
     2101 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc 
     2161 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 
     2221 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     2281 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     2341 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     2401 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     2461 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     2521 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 
     2581 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     2641 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 
     2701 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 
     2761 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 
     2821 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 
     2881 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 
     2941 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     3001 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     3061 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     3121 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     3181 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     3241 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     3301 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     3361 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     3421 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     3481 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     3541 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     3601 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     3661 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     3721 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     3781 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     3841 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     3901 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     3961 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     4021 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     4081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     4141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     4201 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     4261 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     4321 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     4381 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     4441 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     4501 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     4561 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     4621 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     4681 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt 
//
