LOCUS       CN2514_pAAV-3xSP10ins-core2_eHGT367h-minRho*-SYFP2-WPRE3-BGHpA        4627 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...bd-bja6uuy
VERSION     urn.local...bd-bja6uuy
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
                       clones 2,3,4 all good
                       clone 1 low yield
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2514_pAAV-3xSP10ins-core2_eHGT367h-minRho*-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN2514_pAAV-3xSP10ins-core2_eHGT367h-minRho*-SYFP2-WPRE3-BGHpA" 
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COMMENT        (CObList) (CObList) -1 "") 
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COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
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COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2566 0 3063 0 4309 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 6 41 0 52 0 1646 0 1976 0 
COMMENT            1987 0 2443 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 606 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1388 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 644 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2029 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 1978 0 1989 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 1976 0 1987 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 2816 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 158 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 1367 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 9 381 0 652 1 685 1 
COMMENT             1006 1 1189 0 1339 1 2492 1 2689 1 3431 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 53 38 0 143 1 147 0 356 0 
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COMMENT             0 3578 1 3669 1 4031 0 4040 1 4175 0 4285 1 4406 1 4425 1 4552 1 
COMMENT             4580 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 2549 0 3133 0 3506 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 4 799 0 1042 0 3069 0 
COMMENT             4081 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 629 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 633 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 32 12 0 167 0 217 0 267 0 
COMMENT             324 0 371 0 482 0 499 0 624 0 666 0 699 0 771 0 783 0 804 0 1020 0 
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COMMENT             0 3382 0 3445 0 3545 0 4066 0 4323 0 4369 0 4459 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 13 434 1 601 1 614 0 1140 
COMMENT             1 1339 0 3096 0 3100 1 3417 0 3880 1 3881 0 3977 1 3979 0 4065 0 0 
COMMENT             "") #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1373 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 3560 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 1302 0 1876 0 3371 0 
COMMENT             3593 0 0 "") #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2036 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 1 1856 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 1851 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 20 21 0 400 0 791 1 897 0 
COMMENT             1188 0 1214 1 1217 1 1470 1 1588 1 2007 1 2029 0 2166 0 2690 1 3301 
COMMENT             1 3692 0 3995 1 4201 1 4204 1 4294 0 4626 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 12 133 0 428 0 694 0 946 
COMMENT             0 1216 0 1690 0 1898 1 2321 1 2353 0 3278 1 3565 1 3689 1 0 "") 
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COMMENT             "") 
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COMMENT             0 972 0 1062 0 1140 0 1182 0 1233 0 1386 0 1586 0 2309 0 4124 1 0 
COMMENT             "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 1471 1 2898 0 4425 0 
COMMENT             0 "") #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 11 131 0 210 0 260 0 310 0 
COMMENT             625 0 1671 0 1899 0 2183 0 3542 0 3877 0 4130 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 4 349 0 612 0 1652 0 1863 
COMMENT             0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 1267 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 3695 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 628 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 4 498 0 1090 0 1330 0 
COMMENT             3666 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 3131 0 3999 0 4240 1 
COMMENT             4538 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 1973 0 2033 0 2813 
COMMENT             0 3195 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 3669 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 14 606 0 747 1 1021 0 
COMMENT             1036 0 1135 0 1777 0 2032 1 2068 0 2584 1 2674 0 3037 1 3282 0 3477 
COMMENT             1 4529 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 13 23 1 98 0 605 0 1240 
COMMENT             1 1258 1 1772 1 1936 1 2011 0 2587 0 3248 0 3765 1 3931 1 4340 1 0 
COMMENT             "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 3 398 0 676 0 1527 1 0 
COMMENT             "") #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 1 4319 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 774 1 871 0 1195 0 0 
COMMENT             "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 4 2698 0 2740 1 2893 1 
COMMENT             3669 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 2698 0 2740 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 13 24 1 97 0 604 0 1241 
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COMMENT             "") #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 2790 0 2895 0 3903 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 1883 1 2192 0 2893 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 2 3501 0 3675 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1355 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 12 481 0 1257 1 1443 0 2425 
COMMENT             0 3090 0 3260 1 3529 0 3572 0 3690 0 4096 0 4208 1 4221 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1373 0 1928 
COMMENT             0 2013 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 5 549 0 826 1 2759 1 3066 
COMMENT             0 4450 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 619 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 8 415 0 598 1 686 1 1052 
COMMENT             1 2666 1 3289 0 3576 0 3757 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 23 352 0 442 0 609 0 615 0 
COMMENT             788 0 1148 0 1296 0 1334 0 1655 0 1866 0 3055 0 3091 0 3108 0 3366 
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COMMENT             "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 12 465 0 691 0 816 0 838 0 
COMMENT             928 0 1003 0 1057 0 1554 0 1721 0 4464 0 4477 0 4598 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 23 17 0 102 0 153 0 316 0 
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COMMENT             "") #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 40 12 0 15 0 40 0 146 0 
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COMMENT             3683 0 4011 0 4217 0 4220 0 4285 0 4428 0 4583 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 4 365 0 859 0 3316 0 3336 
COMMENT             1 0 "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 36 7 0 17 0 21 0 25 0 94 
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COMMENT             0 4610 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 10 753 1 1017 0 1032 0 1161 
COMMENT             0 1212 0 1584 0 1773 0 2349 0 3082 0 3483 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 23 353 0 443 0 610 0 616 0 
COMMENT             789 0 1149 0 1297 0 1335 0 1656 0 1867 0 3056 0 3092 0 3109 0 3367 
COMMENT             0 3413 0 3431 0 3772 0 3877 0 3889 0 3967 0 3975 0 3986 0 4061 0 0 
COMMENT             "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 6 159 0 1074 0 1356 0 2104 
COMMENT             0 2577 0 3253 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 16 645 0 879 0 909 0 1299 
COMMENT             0 1344 0 1504 0 1859 0 2707 0 2791 0 2896 0 3289 0 3325 0 3403 0 
COMMENT             3413 0 3904 0 4624 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 92 12 0 37 0 51 0 82 0 118 
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COMMENT             0 4323 0 4369 0 4459 0 4557 0 4583 0 4601 0 4612 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 15 32 0 84 0 500 0 591 0 
COMMENT             1249 0 1267 0 1781 0 1945 0 1997 0 2573 0 2808 0 3234 0 3774 0 3940 
COMMENT             0 4349 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 23 351 0 441 0 608 0 614 0 
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COMMENT             0 3411 0 3429 0 3770 0 3875 0 3887 0 3965 0 3973 0 3984 0 4059 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3157 0 3849 0 3868 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 1381 0 1884 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 209 0 259 0 309 0 2936 
COMMENT             1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 894 1 1138 1 1588 0 
COMMENT             3593 0 4421 0 4567 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 8 498 0 799 0 1042 0 
COMMENT             1090 0 1330 0 3069 0 3666 0 4081 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 19 40 0 41 0 51 0 52 0 77 
COMMENT             0 492 0 602 0 673 0 1327 0 1950 0 1975 0 1976 0 1986 0 1987 0 2661 
COMMENT             0 2696 0 3197 0 3548 0 4244 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 482 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 2755 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1396 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 14 485 0 620 0 696 0 990 
COMMENT             0 1017 0 1032 0 1161 0 1368 0 1393 0 1648 0 1659 0 2302 0 3082 0 
COMMENT             4526 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 16 644 0 878 0 908 0 1298 0 
COMMENT             1343 0 1503 0 1858 0 2706 0 2790 0 2895 0 3288 0 3324 0 3402 0 3412 
COMMENT             0 3903 0 4623 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 7 871 0 979 0 1606 0 2128 
COMMENT             1 2197 0 2628 0 2638 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 10 821 0 1222 0 1305 0 
COMMENT              1879 0 2345 0 2758 0 3374 0 3596 0 3613 0 3692 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 40 10 0 13 0 38 0 144 0 
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COMMENT              3342 0 3681 0 4009 0 4215 0 4218 0 4283 0 4426 0 4581 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 8 422 0 585 1 673 1 1039 
COMMENT              1 2653 1 3296 0 3583 0 3764 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3512 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 8 144 1 144 0 717 0 1381 
COMMENT              1 1381 0 1884 0 1884 1 3342 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 24 37 0 82 0 118 0 146 0 
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COMMENT              4612 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "HgaI" "gacgc" 11 16 7 46 1 322 0 1981 0 2645 
COMMENT              0 3203 0 3933 1 4511 1 0 "") 
COMMENT        #108=(CRSite (CStringList) "HhaI" "gcgc" 4 0 33 17 0 19 0 102 0 104 0 
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COMMENT                             "GCAGTGNNN" "CGTCACNNN") 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                             "CTGRAGNNNNNNNNNNNNNNNNN" "GACYTCNNNNNNNNNNNNNNNNN") 
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COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
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COMMENT                              "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT") 
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COMMENT                             1 5 "CCATGG" "GGTACC") 
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COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    163..312
                     /vntifkey="21"
                     /label=3xSP10\core\insulator
     misc_feature    319..478
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=core2_eHGT367h
     misc_feature    485..604
                     /vntifkey="21"
                     /label=Rho\basal\promoter
     misc_feature    645..1364
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1399..1644
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1399..1558
                     /vntifkey="21"
                     /label=gamma
     misc_feature    1564..1644
                     /vntifkey="21"
                     /label=alpha
     misc_feature    1658..1861
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1890..2030
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2122..2428
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    2947..3804
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      3955..4622
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1065 a      1273 c      1211 g      1078 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgccggta ccgaagctac ccctaacaca 
      181 ctattctaca cacagaaaat gctcttcact aggaagctac ccctaacaca ctattctaca 
      241 cacagaaaat gctcttcact aggaagctac ccctaacaca ctattctaca cacagaaaat 
      301 gctcttcact agacgcgttc cagcttaaag gaaaattcca gccccagaga tcttccgcag 
      361 tgagtgacag ctccttaatg tgaggagcag cgtgggcgtg gtgggtggga tgtcagagcc 
      421 atcaaagccc tcatttctga tccctctgtg atgtcggggt tccctggcac gcccactgga 
      481 gctcgattca gccgggagct tagggagggg aggtcacttc ataagggctt ggggggggag 
      541 ttggagccac gagtcgtcca gccggagccc cgtgtggctg tgctccggcc tcagaagcat 
      601 ccccggatcc agatctttcg aagctagcgc taccggtcgc caccatggtg agcaagggcg 
      661 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 
      721 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 
      781 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg 
      841 gctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca 
      901 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 
      961 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 
     1021 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 
     1081 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact 
     1141 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga 
     1201 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 
     1261 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 
     1321 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagtcgac ggcgcgccgc 
     1381 ggccgcgaat tcgatatcat aatcaacctc tggattacaa aatttgtgaa agattgactg 
     1441 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     1501 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag 
     1561 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     1621 tgttgggcac tgacaattcc gtggctcgag agatcttcga ctgtgccttc tagttgccag 
     1681 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 
     1741 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 
     1801 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 
     1861 gagatctcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc 
     1921 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 
     1981 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat 
     2041 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca 
     2101 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     2161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     2221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     2281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt 
     2341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     2401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat 
     2461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     2521 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca 
     2581 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg 
     2641 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 
     2701 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc 
     2761 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 
     2821 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     2881 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     2941 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 
     3001 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 
     3061 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 
     3121 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 
     3181 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 
     3241 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 
     3301 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 
     3361 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 
     3421 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 
     3481 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 
     3541 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 
     3601 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 
     3661 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 
     3721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
     3781 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
     3841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
     3901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 
     3961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 
     4021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 
     4081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 
     4141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 
     4201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 
     4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 
     4321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 
     4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 
     4441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 
     4501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 
     4561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 
     4621 cacatgt 
//
