LOCUS       CN2451-rAAV-eHGT_621h-minBglobin-SYFP2-WPRE3-BGHpA        4643 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...c9y-bb65pqx
VERSION     urn.local...c9y-bb65pqx
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2451-rAAV-eHGT_621h-minBglobin-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            1992 0 2003 0 2459 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 622 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 551 0 1404 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 660 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2045 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 1994 0 2005 0 0 
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COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 1992 0 2003 0 0 
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COMMENT       (CObList 
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COMMENT        #13=(CFSignal (CObList) "SYFP2" 21 0 0 661 1380 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "BGH-pA" 21 0 0 1674 1877 0 (CStringList) 
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COMMENT                          "Gibson/Homology Cloning ligation product." 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/urn.local...c9y-bb65pqx\">urn.local...c9y-bb65pqx</a>" 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..561
                     /created_by="refugio.martinez"
                     /vntifkey="21"
                     /label=eHGT_621h
     misc_feature    568..620
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    661..1380
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1415..1660
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1674..1877
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1906..2046
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2138..2444
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    2963..3820
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      3971..4638
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1111 a      1216 c      1182 g      1134 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgttgctg aaccaattaa ggccagtggt 
      181 accacctgag attttgaaaa taaatcagtg ggtttttatt tcagctgttt ggaaatgact 
      241 aatagagtaa tacaatagat taattttatc taacattaat agctggatgt ataagaaaca 
      301 cccactctct agaaattaag acatgtcacc atagtcttat gacaagagtg agtctcttat 
      361 ttttgaactt tctattgaac agttctcacc atcttaaatc accctttgtt atgagttttt 
      421 aagtgtctta agtagagaag gttaagaaaa tgaatactta tactccttta aatactaatt 
      481 aagaggccct ggtaaccatt tatcagtaat attaatggca tgaaaattga aaaagtatga 
      541 ctcaaaggtg aattcagggc agagctcggg ctgggcataa aagtcagggc agagccatct 
      601 attgcttaca tttgcttctg ggatccagat ctttcgaagc tagcgctacc ggtcgccacc 
      661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 
      721 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 
      781 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc 
      841 ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag 
      901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 
      961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 
     1021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 
     1081 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac 
     1141 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc 
     1201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 
     1261 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc 
     1321 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 
     1381 gtcgacggcg cgccgcggcc gcgaattcga tatcataatc aacctctgga ttacaaaatt 
     1441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     1501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     1561 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     1621 tggacagggg ctcggctgtt gggcactgac aattccgtgg ctcgagagat cttcgactgt 
     1681 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 
     1741 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 
     1801 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 
     1861 agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga 
     1921 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg 
     1981 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc 
     2041 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
     2101 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2161 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     2221 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     2281 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 
     2341 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     2401 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     2461 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     2521 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg 
     2581 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 
     2641 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 
     2701 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 
     2761 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 
     2821 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     2881 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     2941 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     3001 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     3061 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     3121 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     3181 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     3241 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     3301 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     3361 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     3421 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     3481 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     3541 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     3601 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     3661 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     3721 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     3781 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     3841 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     3901 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     3961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     4021 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     4081 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     4141 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     4201 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     4261 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     4321 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     4381 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     4441 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     4501 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     4561 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     4621 ttgctggcct tttgctcaca tgt 
//
