LOCUS       CN1389\pAAV-hI56i(core)-minBG-SYFP2-WPRE3-BGHpA\(JT)        4398 bp    DNA     circular      9-FEB-2018
SOURCE      
  ORGANISM  
COMMENT     rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
            
            clones 2,3,4 all good
            clone 1 low yield 
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12152517|
COMMENT     VNTDBDATE|-12152517|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN1389 pAAV-hI56i(core)-minBG-SYFP2-WPRE3-BGHpA (JT)|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    1661..1801
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      1893..2199
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    2718..3575
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      3726..4393
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    1429..1632
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1170..1415
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1170..1329
                     /vntifkey="21"
                     /label=gamma
     misc_feature    1335..1415
                     /vntifkey="21"
                     /label=alpha
     misc_feature    323..375
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    416..1135
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    156..316
                     /vntifkey="21"
                     /label=hI56i(core)
BASE COUNT     1022 a      1180 c      1146 g      1050 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgttcgcc tttcctgttc ctgaatctaa 
      181 ataaagatgg ctttttagta ttaaaagtgg aagaaaatta caggtaatta tctttgacgg 
      241 taaaaacgct gtaatcagcg ggctacatga aaaattactc taattatggc tgcatttaag 
      301 agaatggacc tgcagggagc tcgggctggg cataaaagtc agggcagagc catctattgc 
      361 ttacatttgc ttctgggatc cagatctttc gaagctagcg ctaccggtcg ccaccatggt 
      421 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 
      481 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 
      541 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 
      601 gaccaccctg ggctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca 
      661 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 
      721 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 
      781 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 
      841 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat 
      901 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca 
      961 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 
     1021 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 
     1081 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagtcga 
     1141 cggcgcgccg cggccgcgaa ttcgatatca taatcaacct ctggattaca aaatttgtga 
     1201 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 
     1261 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 
     1321 atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 
     1381 aggggctcgg ctgttgggca ctgacaattc cgtggctcga gagatcttcg actgtgcctt 
     1441 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 
     1501 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 
     1561 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 
     1621 atagcaggca tgagatctca cgtgcggacc gagcggccgc aggaacccct agtgatggag 
     1681 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 
     1741 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag 
     1801 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt 
     1861 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     1921 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     1981 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
     2041 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg 
     2101 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     2161 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct 
     2221 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
     2281 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca 
     2341 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac 
     2401 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga 
     2461 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac 
     2521 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 
     2581 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
     2641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
     2701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
     2761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
     2821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
     2881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
     2941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
     3001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     3061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     3121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     3181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     3241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     3301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     3361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     3421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     3481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     3541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     3601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     3661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     3721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     3781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     3841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 
     3901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     3961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     4021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     4081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     4141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     4201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     4261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     4321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     4381 ggccttttgc tcacatgt 
//
