LOCUS       CN3445-pAAV-eHGT_873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA        5423 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...2ss-df0z60n
VERSION     urn.local...2ss-df0z60n
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN3445-pAAV-eHGT_873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc 
COMMENT       "CN3445-pAAV-eHGT_873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA" 0 
COMMENT       5423 
COMMENT       (CDBMol 0 0 1 1 1 0 0 522 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5423 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3362 0 3859 0 5105 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 41 0 800 0 2058 0 2442 0 
COMMENT            2772 0 2783 0 3239 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 856 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 1731 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1972 0 2184 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 231 0 319 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 894 0 1959 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2825 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 2060 0 2774 0 2785 0 
COMMENT            0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 2058 0 2772 0 2783 0 
COMMENT            0 "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3612 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 8 902 1 935 1 1256 1 
COMMENT             1439 0 1589 1 3288 1 3485 1 4227 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 57 38 0 132 1 136 0 1114 0 
COMMENT             1155 0 1222 0 1261 0 1356 0 1399 0 1419 1 1572 0 1575 0 1809 0 1835 
COMMENT             1 1860 1 1882 0 2020 0 2174 0 2176 1 2180 0 2369 1 2382 0 2395 0 
COMMENT             2670 1 2679 1 2683 0 2788 1 2838 1 2864 1 2877 0 2914 1 2928 1 2931 
COMMENT             1 2959 0 2986 0 3391 0 3417 0 3427 0 3466 0 3640 1 3687 0 3786 1 
COMMENT             3895 1 3972 1 4016 0 4137 1 4183 0 4374 1 4465 1 4827 0 4836 1 4971 
COMMENT             0 5081 1 5202 1 5221 1 5348 1 5376 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 3345 0 3929 0 4302 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 6 671 0 1049 0 1292 0 
COMMENT             1940 0 3865 0 4877 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 3 879 0 2042 0 2155 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 883 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 31 12 0 233 0 321 0 444 0 
COMMENT             662 0 799 0 874 0 916 0 949 0 1021 0 1033 0 1054 0 1270 0 1318 0 
COMMENT             1429 0 1503 0 1516 0 1603 0 1893 0 2816 0 3045 0 3302 0 3480 0 3499 
COMMENT             0 4178 0 4241 0 4341 0 4862 0 5119 0 5165 0 5255 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 13 851 1 864 0 1390 1 
COMMENT             1589 0 2059 1 3892 0 3896 1 4213 0 4676 1 4677 0 4773 1 4775 0 4861 
COMMENT             0 0 "") #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2169 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 2 461 0 4356 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 1552 0 1927 0 2672 0 
COMMENT             4167 0 4389 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2832 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 1 2652 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 2647 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 23 21 0 442 0 1041 1 1147 
COMMENT             0 1438 0 1464 1 1467 1 1890 0 2073 1 2076 1 2266 1 2384 1 2803 1 
COMMENT             2825 0 2962 0 3486 1 4097 1 4488 0 4791 1 4997 1 5000 1 5090 0 5422 
COMMENT             1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 16 122 0 391 0 838 0 944 
COMMENT             0 1196 0 1466 0 1907 0 2057 0 2144 0 2486 0 2694 1 3117 1 3149 0 
COMMENT             4074 1 4361 1 4485 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 15 970 0 982 0 1030 0 
COMMENT             1111 0 1222 0 1312 0 1390 0 1432 0 1483 0 2060 1 2088 0 2099 1 2382 
COMMENT             0 3105 0 4919 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 15 970 0 982 0 1030 0 
COMMENT             1111 0 1222 0 1312 0 1390 0 1432 0 1483 0 2061 1 2088 0 2100 1 2382 
COMMENT             0 3105 0 4920 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2267 1 3694 0 5221 0 
COMMENT             0 "") #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 9 120 0 875 0 1683 0 2467 0 
COMMENT             2695 0 2979 0 4338 0 4673 0 4926 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 5 493 0 862 0 1766 0 2448 
COMMENT             0 2659 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 1517 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 248 1 4491 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 878 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 4 1340 0 1580 0 1791 0 
COMMENT             4462 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 5 170 0 3927 0 4795 0 
COMMENT             5036 1 5334 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 46 0 2769 0 2829 0 3609 
COMMENT             0 3991 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 904 0 4465 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 639 1 997 1 1271 0 
COMMENT             1286 0 1385 0 1973 0 2009 0 2573 0 2828 1 2864 0 3380 1 3470 0 3833 
COMMENT             1 4078 0 4273 1 5325 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 15 23 1 87 0 405 1 598 
COMMENT             1 1490 1 1508 1 1777 0 2568 1 2732 1 2807 0 3383 0 4044 0 4561 1 
COMMENT             4727 1 5136 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 3 926 0 1676 0 2323 1 0 
COMMENT             "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 4 294 0 689 1 805 1 5115 
COMMENT             0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 1024 1 1121 0 1445 0 0 
COMMENT             "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 6 904 0 1683 1 3494 0 
COMMENT             3536 1 3689 1 4465 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3494 0 3536 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 15 24 1 86 0 406 1 599 
COMMENT             1 1491 1 1509 1 1776 0 2569 1 2733 1 2806 0 3382 0 4043 0 4562 1 
COMMENT             4728 1 5137 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 2 1757 0 1778 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 5 1723 0 1968 0 3586 0 
COMMENT             3691 0 4699 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1915 1 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2679 1 2988 0 3689 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 2 4297 0 4471 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1605 0 1908 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 13 254 1 776 1 1507 1 2239 
COMMENT             0 3221 0 3886 0 4056 1 4325 0 4368 0 4486 0 4892 0 5004 1 5017 1 0 
COMMENT             "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 89 0 2169 0 2724 
COMMENT             0 2809 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1076 1 3555 1 3862 0 
COMMENT             5246 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 2 248 0 869 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 11 383 1 651 0 682 1 936 
COMMENT             1 1302 1 1660 0 1747 0 3462 1 4085 0 4372 0 4553 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 25 496 0 859 0 865 0 1038 
COMMENT             0 1398 0 1546 0 1584 0 1723 0 1769 0 2067 0 2451 0 2662 0 3851 0 
COMMENT             3887 0 3904 0 4162 0 4208 0 4226 0 4567 0 4672 0 4684 0 4762 0 4770 
COMMENT             0 4781 0 4856 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 16 388 0 941 0 1066 0 1088 
COMMENT             0 1178 0 1253 0 1307 0 1671 0 1677 0 2024 0 2094 0 2350 0 2517 0 
COMMENT             5260 0 5273 0 5394 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 22 17 0 91 0 142 0 1224 0 
COMMENT             1542 0 2171 0 2176 0 2182 0 2726 0 2811 0 2904 0 2928 0 2948 0 3324 
COMMENT             0 3435 0 3538 0 3540 0 3640 0 3972 0 4465 0 4795 0 5376 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 45 12 0 15 0 40 0 135 0 
COMMENT             138 0 433 0 1057 0 1138 0 1422 0 1429 0 1480 0 1483 0 1577 0 1863 0 
COMMENT             1881 0 1884 0 2089 0 2092 0 2176 0 2179 0 2182 0 2282 0 2384 0 2400 
COMMENT             0 2682 0 2685 0 2791 0 2816 0 2819 0 2917 0 2931 0 2953 0 3393 0 
COMMENT             3502 0 3789 0 4018 0 4113 0 4140 0 4479 0 4807 0 5013 0 5016 0 5081 
COMMENT             0 5224 0 5379 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 4 542 1 1109 0 4112 0 4132 
COMMENT             1 0 "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 41 7 0 17 0 21 0 25 0 83 
COMMENT             0 87 0 91 0 140 0 380 0 428 0 876 0 1019 0 1052 0 1100 0 1114 0 
COMMENT             1424 0 1431 0 1794 0 1874 0 2084 0 2112 0 2171 0 2386 0 2395 0 2475 
COMMENT             0 2652 0 2726 0 2730 0 2734 0 2803 0 2807 0 2811 0 2821 0 2972 0 
COMMENT             2986 0 3029 0 3405 0 4418 0 4809 0 5369 0 5406 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 14 958 1 1003 1 1267 0 1282 
COMMENT             0 1411 0 1462 0 1732 0 1747 1 2005 0 2380 0 2569 0 3145 0 3878 0 
COMMENT             4279 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 25 497 0 860 0 866 0 1039 0 
COMMENT             1399 0 1547 0 1585 0 1724 0 1770 0 2068 0 2452 0 2663 0 3852 0 3888 
COMMENT             0 3905 0 4163 0 4209 0 4227 0 4568 0 4673 0 4685 0 4763 0 4771 0 
COMMENT             4782 0 4857 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 9 591 0 1324 0 1606 0 1909 
COMMENT             0 2127 0 2146 0 2900 0 3373 0 4049 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 22 275 0 305 0 895 0 1129 
COMMENT             0 1159 0 1549 0 1594 0 1703 0 1724 0 1960 0 1969 0 2300 0 2655 0 
COMMENT             3503 0 3587 0 3692 0 4085 0 4121 0 4199 0 4209 0 4700 0 5420 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 102 12 0 37 0 71 0 107 0 
COMMENT             135 0 148 0 205 0 233 0 321 0 378 0 382 0 401 0 444 0 457 0 511 0 
COMMENT             603 0 662 0 799 0 805 0 829 0 874 0 916 0 949 0 969 0 1021 0 1033 0 
COMMENT             1054 0 1069 0 1092 0 1170 0 1270 0 1318 0 1338 0 1385 0 1429 0 1470 
COMMENT             0 1503 0 1516 0 1603 0 1614 0 1675 0 1776 0 1785 0 1792 0 1824 0 
COMMENT             1881 0 1893 0 1958 0 2015 0 2075 0 2105 0 2135 0 2179 0 2321 0 2411 
COMMENT             0 2416 0 2441 0 2477 0 2682 0 2710 0 2746 0 2777 0 2791 0 2816 0 
COMMENT             3031 0 3045 0 3059 0 3140 0 3244 0 3282 0 3302 0 3403 0 3407 0 3447 
COMMENT             0 3480 0 3499 0 3553 0 4140 0 4178 0 4241 0 4251 0 4341 0 4407 0 
COMMENT             4416 0 4420 0 4446 0 4487 0 4499 0 4862 0 4891 0 4934 0 4945 0 5010 
COMMENT             0 5089 0 5114 0 5119 0 5165 0 5255 0 5353 0 5379 0 5397 0 5408 0 0 
COMMENT             "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 20 32 0 73 0 403 0 414 0 
COMMENT             607 0 1499 0 1517 0 1763 0 1842 0 2130 0 2157 0 2577 0 2741 0 2793 
COMMENT             0 3369 0 3604 0 4030 0 4570 0 4736 0 5145 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 25 495 0 858 0 864 0 1037 0 
COMMENT             1397 0 1545 0 1583 0 1722 0 1768 0 2066 0 2450 0 2661 0 3850 0 3886 
COMMENT             0 3903 0 4161 0 4207 0 4225 0 4566 0 4671 0 4683 0 4761 0 4769 0 
COMMENT             4780 0 4855 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 4 667 0 3953 0 4645 0 4664 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 4 133 0 2073 0 2177 0 2680 
COMMENT             0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 1 3732 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 1144 1 1388 1 2384 0 
COMMENT             4389 0 5217 0 5363 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 10 671 0 1049 0 1292 
COMMENT             0 1340 0 1580 0 1791 0 1940 0 3865 0 4462 0 4877 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 66 0 923 0 
COMMENT             1577 0 2057 0 2058 0 2746 0 2771 0 2772 0 2782 0 2783 0 3457 0 3492 
COMMENT             0 3993 0 4344 0 5040 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 799 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 1673 0 3551 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2192 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 16 249 0 870 0 946 0 1240 
COMMENT             0 1267 0 1282 0 1411 0 1732 0 1996 0 2046 0 2189 0 2444 0 2455 0 
COMMENT             3098 0 3878 0 5322 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 22 274 0 304 0 894 0 1128 0 
COMMENT             1158 0 1548 0 1593 0 1702 0 1723 0 1959 0 1968 0 2299 0 2654 0 3502 
COMMENT             0 3586 0 3691 0 4084 0 4120 0 4198 0 4208 0 4699 0 5419 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 9 1121 0 1229 0 1816 0 
COMMENT             2027 0 2402 0 2924 1 2993 0 3424 0 3434 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 13 1071 0 1472 0 1555 0 
COMMENT              1676 0 1787 0 1930 0 2675 0 3141 0 3554 0 4170 0 4392 0 4409 0 
COMMENT              4488 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 45 10 0 13 0 38 0 133 0 
COMMENT              136 0 431 0 1055 0 1136 0 1420 0 1427 0 1478 0 1481 0 1575 0 1861 
COMMENT              0 1879 0 1882 0 2087 0 2090 0 2174 0 2177 0 2180 0 2280 0 2382 0 
COMMENT              2398 0 2680 0 2683 0 2789 0 2814 0 2817 0 2915 0 2929 0 2951 0 
COMMENT              3391 0 3500 0 3787 0 4016 0 4111 0 4138 0 4477 0 4805 0 5011 0 
COMMENT              5014 0 5079 0 5222 0 5377 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 11 370 1 658 0 669 1 923 
COMMENT              1 1289 1 1667 0 1754 0 3449 1 4092 0 4379 0 4560 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 4308 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 10 133 1 133 0 967 0 
COMMENT              2073 1 2073 0 2177 0 2177 1 2680 0 2680 1 4138 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 30 37 0 71 0 107 0 135 0 
COMMENT              378 0 969 0 1069 0 1385 0 1470 0 1675 0 1785 0 1958 0 2075 0 2105 
COMMENT              0 2135 0 2179 0 2682 0 2710 0 2746 0 2791 0 3140 0 3282 0 3553 0 
COMMENT              4140 0 4407 0 4487 0 4945 0 5379 0 5397 0 5408 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "HgaI" "gacgc" 11 16 6 35 1 2777 0 3441 0 
COMMENT              3999 0 4729 1 5307 1 0 "") 
COMMENT        #108=(CRSite (CStringList) "HhaI" "gcgc" 4 0 40 17 0 19 0 91 0 93 0 880 
COMMENT              0 1185 0 1226 0 1542 0 1849 0 1872 0 1878 0 2001 0 2029 0 2043 0 
COMMENT              2082 0 2156 0 2171 0 2173 0 2726 0 2728 0 2811 0 2813 0 2831 0 
COMMENT              2906 0 2919 0 2928 0 2950 0 2976 0 2984 0 3437 0 3540 0 3640 0 
COMMENT              3972 0 4309 0 4402 0 4795 0 4904 0 5078 0 5178 0 5245 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "HinfI" "gantc" 2 0 8 269 0 360 0 1666 0 1761 
COMMENT              0 3180 0 3202 0 4532 0 5049 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "HinP1I" "gcgc" 2 0 40 15 0 17 0 89 0 91 0 
COMMENT              878 0 1183 0 1224 0 1540 0 1847 0 1870 0 1876 0 1999 0 2027 0 2041 
COMMENT              0 2080 0 2154 0 2169 0 2171 0 2724 0 2726 0 2809 0 2811 0 2829 0 
COMMENT              2904 0 2917 0 2926 0 2948 0 2974 0 2982 0 3435 0 3538 0 3638 0 
COMMENT              3970 0 4307 0 4400 0 4793 0 4902 0 5076 0 5176 0 5243 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "HpaII" "ccgg" 2 0 26 42 0 68 0 884 0 924 0 
COMMENT              987 0 1047 0 1578 0 1758 0 1779 0 1941 0 2059 0 2747 0 2773 0 2784 
COMMENT              0 3028 0 3459 0 3493 0 3994 0 4236 0 4346 0 4413 0 4447 0 4851 0 
COMMENT              5041 0 5067 0 5214 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "HphI" "ggtga" 14 13 15 913 1 1243 0 1267 0 
COMMENT              1345 1 1849 0 2044 1 2131 1 3132 0 3511 1 3520 1 3804 1 3839 0 
COMMENT              4045 1 4461 0 4688 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "Hpy99I" "cgwcg" 6 0 7 51 0 1208 0 1469 0 
COMMENT              2771 0 4266 0 4529 0 5323 0 0 "") 
COMMENT        #115=(CRSite (CStringList) "HpyAV" "ccttc" 12 11 15 255 0 412 1 1160 1 
COMMENT              1267 1 1825 1 2473 0 2514 1 2515 0 3016 0 3807 0 4166 1 4419 0 
COMMENT              4874 1 4932 0 5184 1 0 "") 
COMMENT        #116=(CRSite (CStringList) "HpyCH4I" "catg" 4 0 22 277 0 307 0 897 0 
COMMENT              1131 0 1161 0 1551 0 1596 0 1705 0 1726 0 1962 0 1971 0 2302 0 
COMMENT              2657 0 3505 0 3589 0 3694 0 4087 0 4123 0 4201 0 4211 0 4702 0 
COMMENT              5422 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "HpyCH4IV" "acgt" 2 0 13 1173 0 1344 0 2666 0 
COMMENT              2883 0 3021 0 3131 0 3174 0 3186 0 3345 0 3609 0 3929 0 4302 0 
COMMENT              4718 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "HpyCH4V" "tgca" 3 0 18 5 0 238 0 466 0 781 0 
COMMENT              1041 0 1102 0 1426 0 2565 0 2823 0 3364 0 3502 0 3861 0 4105 0 
COMMENT              4193 0 4386 0 4476 0 4811 0 5107 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 2828 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "MaeIII" "gtnac" 1 0 15 771 0 1078 0 1567 0 
COMMENT              1837 0 2942 0 2954 0 3482 0 3870 0 4058 0 4211 0 4269 0 4600 0 
COMMENT              4883 0 4999 0 5062 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "MboI" "gatc" 1 0 25 493 0 856 0 862 0 1035 0 
COMMENT              1395 0 1543 0 1581 0 1720 0 1766 0 2064 0 2448 0 2659 0 3848 0 
COMMENT              3884 0 3901 0 4159 0 4205 0 4223 0 4564 0 4669 0 4681 0 4759 0 
COMMENT              4767 0 4778 0 4853 0 0 "") 
COMMENT        #123=(CRSite (CStringList) "MboII" "gaaga" 14 13 14 278 0 1138 1 1183 1 
COMMENT              1186 1 1381 0 1864 0 2443 1 2652 0 2993 1 3749 0 3858 0 3936 0 
COMMENT              4691 0 4762 1 0 "") 
COMMENT        #124=(CRSite (CStringList) "MfeI" "caattg" 2 0 0 0 "") 
COMMENT        #125=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 140 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "MlyI" "gagtc" 11 11 5 1755 1 3189 0 3196 1 
COMMENT              4541 0 5043 1 0 "") 
COMMENT        #127=(CRSite (CStringList) "MmeI" "tccrac" 27 25 3 3157 1 5026 1 5210 1 
COMMENT              0 "") 
COMMENT        #128=(CRSite (CStringList) "MnlI" "cctc" 12 11 41 27 1 82 0 91 1 194 1 
COMMENT              495 0 614 0 754 1 783 1 904 1 985 1 991 1 1085 0 1222 1 1234 1 
COMMENT              1285 1 1405 1 1654 1 1766 1 1775 1 2005 1 2065 0 2116 1 2159 1 
COMMENT              2214 0 2344 0 2504 0 2549 1 2624 1 2727 0 2736 1 2802 0 3105 0 
COMMENT              3505 1 3564 0 4158 1 4364 1 4511 0 4592 0 4992 0 5242 1 5316 0 0 
COMMENT              "") 
COMMENT        #129=(CRSite (CStringList) "MscI" "tggcca" 4 0 4 107 0 378 0 2105 0 2710 
COMMENT              0 0 "") 
COMMENT        #130=(CRSite (CStringList) "MseI" "ttaa" 2 0 26 366 0 461 0 569 0 616 0 
COMMENT              621 0 666 0 720 0 2244 0 2285 0 2922 0 3193 0 3291 0 3308 0 3319 0 
COMMENT              3331 0 3342 0 3399 0 3580 0 3952 0 4317 0 4356 0 4591 0 4644 0 
COMMENT              4658 0 4663 0 4715 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 8 12 0 2176 0 2397 0 
COMMENT              2816 0 3429 0 3895 0 4836 0 5081 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "NaeI" "gccggc" 4 0 1 3029 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 2829 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "NciI" "ccsgg" 3 0 17 42 0 43 0 68 0 925 0 
COMMENT              1579 0 2059 0 2060 0 2748 0 2773 0 2774 0 2784 0 2785 0 3459 0 
COMMENT              3494 0 3995 0 4346 0 5042 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 783 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 1 3027 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 874 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "NlaIII" "catg" 5 0 22 278 0 308 0 898 0 1132 
COMMENT              0 1162 0 1552 0 1597 0 1706 0 1727 0 1963 0 1972 0 2303 0 2658 0 
COMMENT              3506 0 3590 0 3695 0 4088 0 4124 0 4202 0 4212 0 4703 0 5423 0 0 
COMMENT              "") 
COMMENT        #139=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 3 133 0 2177 0 2680 0 0 
COMMENT              "") #140=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #143=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 5419 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "PleI" "gagtc" 10 11 5 1755 1 3188 0 3196 1 
COMMENT              4540 0 5043 1 0 "") 
COMMENT        #145=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 1 2667 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Ppu10I" "atgcat" 2 0 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "PsiI" "ttataa" 4 0 3 479 0 1716 0 3260 0 0 
COMMENT              "") #149=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 4162 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 12 0 2816 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "RleAI" "cccaca" 19 16 0 0 "") 
COMMENT        #153=(CRSite (CStringList) "RsaI" "gtac" 3 0 9 592 0 1325 0 1607 0 1910 
COMMENT              0 2128 0 2147 0 2901 0 3374 0 4050 0 0 "") 
COMMENT        #154=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 801 0 0 "") 
COMMENT        #155=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 2177 0 0 "") 
COMMENT        #156=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #157=(CRSite (CStringList) "SapI" "gctcttc" 9 12 0 0 "") 
COMMENT        #158=(CRSite (CStringList) "Sau96I" "ggncc" 2 0 13 1068 0 1469 0 1552 0 
COMMENT              1673 0 1784 0 1927 0 2672 0 3138 0 3551 0 4167 0 4389 0 4406 0 
COMMENT              4485 0 0 "") 
COMMENT        #159=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 2 7 0 2825 0 0 "") 
COMMENT        #160=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4050 0 0 "") 
COMMENT        #161=(CRSite (CStringList) "SciI" "ctcgag" 4 0 1 2444 0 0 "") 
COMMENT        #162=(CRSite (CStringList) "SelI" "cgcg" 1 0 22 15 0 89 0 140 0 1222 0 
COMMENT              1540 0 2169 0 2174 0 2180 0 2724 0 2809 0 2902 0 2926 0 2946 0 
COMMENT              3322 0 3433 0 3536 0 3538 0 3638 0 3970 0 4463 0 4793 0 5374 0 0 
COMMENT              "") 
COMMENT        #163=(CRSite (CStringList) "SfaNI" "gcatc" 11 15 16 636 1 994 1 1272 0 
COMMENT              1287 0 1386 0 1974 0 2010 0 2574 0 2825 1 2865 0 3377 1 3471 0 
COMMENT              3830 1 4079 0 4270 1 5322 1 0 "") 
COMMENT        #164=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 1 2830 0 0 "") 
COMMENT        #165=(CRSite (CStringList) "SimI" "gggtc" 3 6 8 1023 1 1218 1 1525 1 
COMMENT              2512 1 3098 1 4459 0 4745 1 5228 0 0 "") 
COMMENT        #166=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 6 149 0 2442 0 3906 0 
COMMENT              4774 0 5051 0 5313 0 0 "") 
COMMENT        #167=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 0 0 "") 
COMMENT        #168=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #169=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #170=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 3 43 0 2774 0 2785 0 0 
COMMENT              "") #171=(CRSite (CStringList) "Sse232I" "cgccggcg" 3 0 0 0 "") 
COMMENT        #172=(CRSite (CStringList) "SspD5I" "ggtga" 14 14 15 912 1 1243 0 1267 0 
COMMENT              1344 1 1849 0 2043 1 2130 1 3132 0 3510 1 3519 1 3803 1 3839 0 
COMMENT              4044 1 4461 0 4688 0 0 "") 
COMMENT        #173=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 3340 0 3726 0 0 "") 
COMMENT        #174=(CRSite (CStringList) "Sth132I" "cccg" 9 13 52 34 1 41 1 48 0 74 0 
COMMENT              793 1 916 1 1065 0 1120 0 1128 0 1170 0 1228 0 1479 0 1491 0 1570 
COMMENT              1 1815 0 1820 0 2026 0 2051 1 2065 0 2320 0 2401 0 2506 0 2739 1 
COMMENT              2765 1 2772 0 2776 1 2779 0 2790 0 2923 1 2992 0 3044 0 3045 1 
COMMENT              3232 1 3416 0 3423 0 3433 0 3435 1 3465 0 3485 1 3618 1 3928 0 
COMMENT              3986 1 3989 0 4352 0 4511 0 4519 1 4756 0 5033 1 5077 1 5086 1 
COMMENT              5194 0 5285 1 0 "") 
COMMENT        #175=(CRSite (CStringList) "StsI" "ggatg" 16 20 11 369 1 659 0 668 1 922 
COMMENT              1 1288 1 1668 0 1755 0 3448 1 4093 0 4380 0 4561 0 0 "") 
COMMENT        #176=(CRSite (CStringList) "StuI" "aggcct" 4 0 0 0 "") 
COMMENT        #177=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #178=(CRSite (CStringList) "TaiI" "acgt" 5 0 13 1176 0 1347 0 2669 0 
COMMENT              2886 0 3024 0 3134 0 3177 0 3189 0 3348 0 3612 0 3932 0 4305 0 
COMMENT              4721 0 0 "") 
COMMENT        #179=(CRSite (CStringList) "TaqI" "tcga" 2 0 16 248 0 869 0 945 0 1239 0 
COMMENT              1266 0 1281 0 1410 0 1731 0 1995 0 2045 0 2188 0 2443 0 2454 0 
COMMENT              3097 0 3877 0 5321 0 0 "") 
COMMENT        #180=(CRSite (CStringList) "Tsp45I" "gtsac" 1 0 7 1078 0 1567 0 1837 0 
COMMENT              2954 0 3482 0 4058 0 4269 0 0 "") 
COMMENT        #181=(CRSite (CStringList) "Tsp509I" "aatt" 1 0 16 474 0 480 0 618 0 628 
COMMENT              0 668 0 1972 0 2184 0 2217 0 2431 0 2560 0 3315 0 3326 0 3352 0 
COMMENT              4098 0 4353 0 4659 0 0 "") 
COMMENT        #182=(CRSite (CStringList) "TspDTI" "atgaa" 17 15 14 373 0 384 0 485 0 
COMMENT              725 1 729 1 733 1 737 1 741 1 1145 0 1985 0 2314 1 4261 0 4564 0 
COMMENT              4641 1 0 "") 
COMMENT        #183=(CRSite (CStringList) "TspGWI" "acgga" 17 15 4 1722 0 2425 1 3750 1 
COMMENT              4092 0 0 "") 
COMMENT        #184=(CRSite (CStringList) "TspRI" "castg" 8 0 20 36 0 81 0 228 0 261 0 
COMMENT              418 0 549 0 783 0 1109 0 1641 0 2431 0 2540 0 2745 0 2801 0 4112 0 
COMMENT              4139 0 4486 0 4591 0 4740 0 5011 0 5024 0 0 "") 
COMMENT        #185=(CRSite (CStringList) "UthSI" "cccggg" 3 0 4 42 0 2059 0 2773 0 
COMMENT              2784 0 0 "") 
COMMENT        #186=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #187=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2442 0 0 "") 
COMMENT        #188=(CRSite (CStringList) "ZraI" "gacgtc" 4 0 1 3610 0 0 "")) 
COMMENT       (CObList 
COMMENT        #189=(CFSignal (CObList) "L-ITR" 21 0 0 1 130 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"L-ITR\"" "/ApEinfo_label=\"L-ITR\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "1..130") 
COMMENT        #190=(CFSignal (CObList) "Beta Globin minimal promoter" 21 0 0 802 854 0 
COMMENT              (CStringList) 
COMMENT              (CStringList "/locus_tag=\"Beta\\Globin\\minimal\\promoter\"" 
COMMENT               "/ApEinfo_label=\"Beta\\Globin\\minimal\\promoter\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "802..854") 
COMMENT        #191=(CFSignal (CObList) "SYFP2" 21 0 0 895 1609 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"SYFP2\"" "/ApEinfo_label=\"SYFP2\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "895..1609") 
COMMENT        #192=(CFSignal (CObList) "P2A" 21 0 0 1612 1689 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"P2A\"" "/ApEinfo_label=\"P2A\"" 
COMMENT               "/ApEinfo_fwdcolor=\"cyan\"" "/ApEinfo_revcolor=\"green\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "1612..1689") 
COMMENT        #193=(CFSignal (CObList) "3xFLAG" 4 0 0 1690 1755 0 (CStringList) 
COMMENT              (CStringList "/codon_start=1" 
COMMENT               "/product=\"three tandem FLAG epitope tags, followed by an" 
COMMENT               "enterokinase cleavage site\"" "/locus_tag=\"3xFLAG\"" 
COMMENT               "/ApEinfo_label=\"3xFLAG\"" "/ApEinfo_fwdcolor=\"#e9d024\"" 
COMMENT               "/ApEinfo_revcolor=\"#e9d024\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "1690..1755") 
COMMENT        #194=(CFSignal (CObList) "10 a.a. linker" 21 0 0 1756 1785 0 
COMMENT              (CStringList) 
COMMENT              (CStringList "/locus_tag=\"10\\a.a.\\linker\"" 
COMMENT               "/ApEinfo_label=\"10\\a.a.\\linker\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "1756..1785") 
COMMENT        #195=(CFSignal (CObList) "H2B" 21 0 0 1786 2160 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"H2B\"" "/ApEinfo_label=\"H2B\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "1786..2160") 
COMMENT        #196=(CFSignal (CObList) "WPRE3" 21 0 0 2195 2440 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"WPRE3\"" "/ApEinfo_label=\"WPRE3\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "2195..2440") 
COMMENT        #197=(CFSignal (CObList) "gamma" 21 0 0 2195 2354 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"gamma\"" "/ApEinfo_label=\"gamma\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "2195..2354") 
COMMENT        #198=(CFSignal (CObList) "alpha" 21 0 0 2360 2440 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"alpha\"" "/ApEinfo_label=\"alpha\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "2360..2440") 
COMMENT        #199=(CFSignal (CObList) "BGH-pA" 21 0 0 2454 2657 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"BGH-pA\"" "/ApEinfo_label=\"BGH-pA\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "2454..2657") 
COMMENT        #200=(CFSignal (CObList) "R-ITR" 21 0 0 2686 2826 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"R-ITR\"" "/ApEinfo_label=\"R-ITR\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "2686..2826") 
COMMENT        #201=(CFSignal (CObList) "f1 Origin" 33 0 0 2918 3224 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"f1\\Origin\"" 
COMMENT               "/ApEinfo_label=\"f1\\Origin\"" "/ApEinfo_fwdcolor=\"#999999\"" 
COMMENT               "/ApEinfo_revcolor=\"#999999\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "2918..3224") 
COMMENT        #202=(CFSignal (CObList) "AmpR" 21 0 0 3743 4600 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"AmpR\"" "/ApEinfo_label=\"AmpR\"" 
COMMENT               "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "3743..4600") 
COMMENT        #203=(CFSignal (CObList) "pUC ori" 33 0 0 4751 5418 0 (CStringList) 
COMMENT              (CStringList "/locus_tag=\"pUC\\ori\"" 
COMMENT               "/ApEinfo_label=\"pUC\\ori\"" "/ApEinfo_fwdcolor=\"#999999\"" 
COMMENT               "/ApEinfo_revcolor=\"#999999\"" 
COMMENT               "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT               "width 5 offset 0\"") 1 1 1 1 "4751..5418") 
COMMENT        #204=(CFSignal (CObList) "eHGT_873m" 21 0 0 145 795 0 (CStringList) 
COMMENT              (CStringList "/created_by=\"refugio.martinez\"") 1 1 1 1 
COMMENT              "145..795")) (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CTextView 0 
COMMENT        #205=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT              (CObjectList 
COMMENT               #206=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                      "CN3445-pAAV-eHGT_873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA" 
COMMENT                      2) 5 "" 0 4) 
COMMENT               #207=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #208=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "DNA 'CN3445-pAAV-eHGT_873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA'" 
COMMENT                             1) 
COMMENT                       #209=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Gibson/Homology Cloning ligation product." 1) 
COMMENT                       #210=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                             1) 
COMMENT                       #211=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Creation Date: ? (no data)" 1) 
COMMENT                       #212=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modification Date: ? (no data)" 1) 
COMMENT                       #213=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 5423 bp" 1) 
COMMENT                       #214=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "storage type: Basic" 1) 
COMMENT                       #215=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "form: Circular" 1))) "General Description") 
COMMENT               #216=(CFolderPar 
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COMMENT                              "BciVI: 3 sites") 12 11 "GTATCCNNNNNNN" 
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COMMENT                              "BglII: 5 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                              "BmrI: 2 sites") 11 10 "ACTGGGNNNNNN" 
COMMENT                             "TGACCCNNNNNN") 
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COMMENT                              "BpmI: 4 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BpuEI: 5 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaHI: 5 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
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COMMENT                                             " N3: 2323 (on complementary strand)" 
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COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
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COMMENT                              "BseYI: 4 sites") 1 5 "CCCAGC" "GGGTCG") 
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COMMENT                              "BsgI: 3 sites") 22 20 "GTGCAGNNNNNNNNNNNNNNNNN" 
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COMMENT                             "GTCTCNNNNNN" "CAGAGNNNNNN") 
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COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
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COMMENT                             "CTTACGNN") 
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COMMENT                               (CObjectList)) "BspCNI: 15 sites") 14 12 
COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
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COMMENT                              "BspEI: 2 sites") 1 5 "TCCGGA" "AGGCCT") 
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COMMENT                              "BspHI: 5 sites") 1 5 "TCATGA" "AGTACT") 
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COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
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COMMENT                              "BsrBI: 3 sites") 3 3 "CCGCTC" "GGCGAG") 
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COMMENT                                       #588=(CLinePar 
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COMMENT                             "GCAATGNNN" "CGTTACNNN") 
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COMMENT                              "BsrGI: 2 sites") 1 5 "TGTACA" "ACATGT") 
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COMMENT                                       #597=(CLinePar 
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COMMENT                                       #598=(CLinePar 
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COMMENT                                       #599=(CLinePar 
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COMMENT                                       #600=(CLinePar 
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COMMENT                              "BssHII: 5 sites") 1 5 "GCGCGC" "CGCGCG") 
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COMMENT                                             " N1: 1076 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #604=(CLinePar 
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COMMENT                                             " N2: 3555 (on complementary strand)" 
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COMMENT                                       #606=(CLinePar 
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COMMENT                              "BssSI: 4 sites") 1 5 "CACGAG" "GTGCTC") 
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COMMENT                                       #610=(CLinePar 
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COMMENT                              "BstBI: 2 sites") 2 4 "TTCGAA" "AAGCTT") 
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COMMENT                             "CCTACNNN") 
COMMENT                       #613=(CRSFolderPar 
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COMMENT                               (CObjectList)) "BstKTI: 25 sites") 3 1 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #614=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99949184 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstNI: 16 sites") 2 3 "CCWGG" 
COMMENT                             "GGWCC") 
COMMENT                       #615=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99949024 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstUI: 22 sites") 2 2 "CGCG" 
COMMENT                             "GCGC") 
COMMENT                       #616=(CRSFolderPar 
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COMMENT                             "CGNCG") 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
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COMMENT                             "CCCGCNNNNNNN" "GGGCGNNNNNNN") 
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COMMENT                             "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
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COMMENT                              "FspI: 1 site") 3 3 "TGCGCA" "ACGCGT") 
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COMMENT                             "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN") 
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COMMENT                              "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                              (CGroupPar (CParagraph 99950848 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HphI: 15 sites") 13 12 
COMMENT                             "GGTGANNNNNNNNN" "CCACTNNNNNNNNN") 
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COMMENT                              "KasI: 1 site") 1 5 "GGCGCC" "CCGCGG") 
COMMENT                       #698=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 99950624 (8 0) 1 1 1 0 178) 
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COMMENT                       #700=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 99950240 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MboI: 25 sites") 1 5 "NGATCN" 
COMMENT                             "NCTAGN") 
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COMMENT                              (CGroupPar (CParagraph 99950304 (8 0) 1 1 1 0 178) 
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COMMENT                             "GAAGANNNNNNNNN" "CTTCTNNNNNNNNN") 
COMMENT                       #702=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99950112 (8 0) 1 1 1 0 178) 
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COMMENT                             "GTTAAC") 
COMMENT                       #703=(CRSFolderPar 
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COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 140 " 1))))) 
COMMENT                              "MluI: 1 site") 1 5 "ACGCGT" "TGCGCA") 
COMMENT                       #706=(CRSFolderPar 
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COMMENT                                      (CObjectList 
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COMMENT                                             " N1: 1755 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #709=(CLinePar 
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COMMENT                                              191) " N2: 3189 " 1) 
COMMENT                                       #710=(CLinePar 
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COMMENT                                              191) 
COMMENT                                             " N3: 3196 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #711=(CLinePar 
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COMMENT                                              191) " N4: 4541 " 1) 
COMMENT                                       #712=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #126# 5) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N5: 5043 (on complementary strand)" 
COMMENT                                             1))))) "MlyI: 5 sites") 10 10 
COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
COMMENT                       #713=(CRSFolderPar 
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COMMENT                                             " N1: 3157 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #716=(CLinePar 
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COMMENT                                              191) 
COMMENT                                             " N2: 5026 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #717=(CLinePar 
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COMMENT                                              191) 
COMMENT                                             " N3: 5210 (on complementary strand)" 
COMMENT                                             1))))) "MmeI: 3 sites") 26 24 
COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
COMMENT                             "AGGYTGNNNNNNNNNNNNNNNNNNNNN") 
COMMENT                       #718=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99950752 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MnlI: 41 sites") 11 10 
COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
COMMENT                       #719=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99950880 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                       #722=(CLinePar 
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COMMENT                                       #723=(CLinePar 
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COMMENT                                       #724=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #129# 4) 1 2 2 0 
COMMENT                                              191) " N4: 2710 " 1))))) 
COMMENT                              "MscI: 4 sites") 3 3 "TGGCCA" "ACCGGT") 
COMMENT                       #725=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99950944 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MseI: 26 sites") 1 3 "TTAA" 
COMMENT                             "AATT") 
COMMENT                       #726=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 99950976 (8 0) 1 1 1 0 178) 
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COMMENT                             "GKCGMC") 
COMMENT                       #727=(CRSFolderPar 
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COMMENT                              "NaeI: 1 site") 3 3 "GCCGGC" "CGGCCG") 
COMMENT                       #730=(CRSFolderPar 
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COMMENT                               (CObjectList 
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COMMENT                              "NarI: 1 site") 2 4 "GGCGCC" "CCGCGG") 
COMMENT                       #733=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 99951072 (8 0) 1 1 1 0 178) 
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FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    125^126
                     /Original_Bases="NAGCGCGCNNGAGNAGGAGTGGCCAACTCCATCACTAGGGG
                     "
                     /vntifkey="21"
                     /label=NAGCGCGCNNGAGNAGGAGTGGCCAACTCCATCACTAGGGG
                     /note="Geneious type: Editing History Deletion"
     misc_feature    145..795
                     /created_by="refugio.martinez"
                     /vntifkey="21"
                     /label=eHGT_873m
     misc_feature    802..854
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    819^820
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACATTTGCTTCTGGGATCC
                     AGNNNNNNNNNNNNNNNNNNNN"
                     NNNNNNNNNNNNN"
                     /vntifkey="21"
                     /label=AGGGCAGAGCCATCTATTGCTTACATTTGCTTCTGGGATCCAGNNNNNNN
                     /note="Geneious type: Editing History Deletion"
     misc_feature    895..1609
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1612..1689
                     /locus_tag="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=P2A
     CDS             1690..1755
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an
                     enterokinase cleavage site"
                     /locus_tag="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="4"
                     /label=3xFLAG
     misc_feature    1756..1785
                     /locus_tag="10\a.a.\linker"
                     /ApEinfo_label="10\a.a.\linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=10\a.a.\linker
     misc_feature    1786..2160
                     /locus_tag="H2B"
                     /ApEinfo_label="H2B"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=H2B
     misc_feature    2195..2440
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2195..2354
                     /locus_tag="gamma"
                     /ApEinfo_label="gamma"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=gamma
     misc_feature    2360..2440
                     /locus_tag="alpha"
                     /ApEinfo_label="alpha"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=alpha
     misc_feature    2454..2657
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2686..2826
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2918..3224
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3743..4600
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4751..5418
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1308 a      1423 c      1396 g      1296 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtgggctt gagaaatatc atcacaccat tggaagtctt 
      181 acaacttgat gtgaacacag agggctttcg gtagaaatat cactgtagga agctttgcag 
      241 acaccttcga acccagtgga agtagaagat tcccatgtct gtgttctcta ttcccagcac 
      301 cagcatggat ttctccaaag ctttgtaggg cagaactctg aatgtttatt tctctgatga 
      361 atccttaatg aaagtggcca gccatcctgg ggagaacaag ccttagttcc cactgagaag 
      421 gatagcaagc agcataagag tagctgatgt caaaggctat taatgcaaat gaaaattata 
      481 attcctcatc aagatctgct cccagacagg ctaatcattg tgccacctgt ttacagaaac 
      541 acactgctaa tcagtcagta aaatctttta agtttctgtg cttctctgtg tacgattttt 
      601 agcctctgag agcattaaat taacttcaat tagtaaatct aatggatgct gaagtgttga 
      661 gcttttaaat tcaatggaaa acatcctgct ggggaaaaac attatttcta atgagatttt 
      721 aagtatttat ttattcattc attcattcat tcattcaaag agggatatat gttaccagtg 
      781 catatgtgga ggtcagagct cgggctgggc ataaaagtca gggcagagcc atctattgct 
      841 tacatttgct tctgggatcc agatctttcg aagctagcgc taccggtcgc caccatggtc 
      901 tccaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgat 
      961 gtcaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     1021 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     1081 accaccctgg gctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac 
     1141 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaaa 
     1201 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     1261 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     1321 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 
     1381 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac 
     1441 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     1501 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     1561 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggctctggt 
     1621 gctaccaact tctcactgtt gaaacaggca ggggatgtag aggagaatcc agggcctggt 
     1681 gctagtggag actacaaaga ccatgacgga gattataaag atcatgacat cgattacaag 
     1741 gatgacgatg acaagtccgg actcagatct ggaggctccg gaggcccaga gccagcgaag 
     1801 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc 
     1861 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg 
     1921 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt 
     1981 gtgaacgaca ttttcgagcg catcgcagga gaggcttccc gcctggcgca ttacaacaag 
     2041 cgctcgacca tcacctcccg ggagatccag acggccgtgc gcctgctgct gcctggggag 
     2101 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag 
     2161 taatgaggcg cgccgcggcc gcgaattcga tatcataatc aacctctgga ttacaaaatt 
     2221 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     2281 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     2341 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     2401 tggacagggg ctcggctgtt gggcactgac aattccgtgg ctcgagagat cttcgactgt 
     2461 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 
     2521 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 
     2581 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 
     2641 agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga 
     2701 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg 
     2761 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc 
     2821 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
     2881 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2941 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     3001 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     3061 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 
     3121 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     3181 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     3241 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     3301 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg 
     3361 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 
     3421 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 
     3481 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 
     3541 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 
     3601 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     3661 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     3721 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     3781 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     3841 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     3901 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     3961 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     4021 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     4081 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     4141 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     4201 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     4261 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     4321 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     4381 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     4441 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     4501 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     4561 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     4621 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     4681 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     4741 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     4801 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     4861 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     4921 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     4981 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     5041 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     5101 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     5161 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     5221 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     5281 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     5341 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     5401 ttgctggcct tttgctcaca tgt 
//
