LOCUS       CN2689_rAAV-eHGT_682h-minBglobin-SYFP2-WPRE3-BGHpA        4986 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...30o-c1qk8l3
VERSION     urn.local...30o-c1qk8l3
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2689_rAAV-eHGT_682h-minBglobin-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN2689_rAAV-eHGT_682h-minBglobin-SYFP2-WPRE3-BGHpA" 0 4986 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1667724391 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4986 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2925 0 3422 0 4668 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 41 0 52 0 909 0 2005 0 
COMMENT            2335 0 2346 0 2802 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 965 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1747 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 738 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 277 0 1003 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2388 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2337 0 2348 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2335 0 2346 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 1 391 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 2 324 0 574 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3175 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 1726 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 9 722 0 1011 1 1044 1 
COMMENT             1365 1 1548 0 1698 1 2851 1 3048 1 3790 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 52 38 0 143 1 147 0 1223 0 
COMMENT             1264 0 1331 0 1370 0 1465 0 1508 0 1528 1 1681 0 1684 0 1737 0 1739 
COMMENT             1 1743 0 1932 1 1945 0 1958 0 2233 1 2242 1 2246 0 2351 1 2401 1 
COMMENT             2427 1 2440 0 2477 1 2491 1 2494 1 2522 0 2549 0 2954 0 2980 0 2990 
COMMENT             0 3029 0 3203 1 3250 0 3349 1 3458 1 3535 1 3579 0 3700 1 3746 0 
COMMENT             3937 1 4028 1 4390 0 4399 1 4534 0 4644 1 4765 1 4784 1 4911 1 4939 
COMMENT             1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 2908 0 3492 0 3865 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 4 1158 0 1401 0 3428 0 
COMMENT             4440 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 988 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 992 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 29 12 0 712 0 740 0 770 0 
COMMENT             908 0 983 0 1025 0 1058 0 1130 0 1142 0 1163 0 1379 0 1427 0 1538 0 
COMMENT             1612 0 1625 0 1712 0 2379 0 2608 0 2865 0 3043 0 3062 0 3741 0 3804 
COMMENT             0 3904 0 4425 0 4682 0 4728 0 4818 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 12 960 1 973 0 1499 1 
COMMENT             1698 0 3455 0 3459 1 3776 0 4239 1 4240 0 4336 1 4338 0 4424 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1732 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 3919 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 6 426 0 656 0 1661 0 2235 
COMMENT             0 3730 0 3952 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2395 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 1 2215 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 2210 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 20 21 0 616 0 1150 1 1256 
COMMENT             0 1547 0 1573 1 1576 1 1829 1 1947 1 2366 1 2388 0 2525 0 3049 1 
COMMENT             3660 1 4051 0 4354 1 4560 1 4563 1 4653 0 4985 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 15 133 0 322 1 326 1 484 
COMMENT             1 947 0 1053 0 1305 0 1575 0 2049 0 2257 1 2680 1 2712 0 3637 1 
COMMENT             3924 1 4048 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 13 1079 0 1091 0 1139 0 
COMMENT             1220 0 1331 0 1421 0 1499 0 1541 0 1592 0 1745 0 1945 0 2668 0 4482 
COMMENT             1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 13 1079 0 1091 0 1139 0 
COMMENT             1220 0 1331 0 1421 0 1499 0 1541 0 1592 0 1745 0 1945 0 2668 0 4483 
COMMENT             1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 1830 1 3257 0 4784 0 
COMMENT             0 "") #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 507 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 9 131 0 783 0 984 0 2030 0 
COMMENT             2258 0 2542 0 3901 0 4236 0 4489 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 3 971 0 2011 0 2222 0 0 
COMMENT             "") #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 1626 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 354 1 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4054 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 987 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 4 162 1 1449 0 1689 0 
COMMENT             4025 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 3490 0 4358 0 4599 1 
COMMENT             4897 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2332 0 2392 0 3172 
COMMENT             0 3554 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 4028 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 14 768 0 1106 1 1380 0 
COMMENT             1395 0 1494 0 2136 0 2391 1 2427 0 2943 1 3033 0 3396 1 3641 0 3836 
COMMENT             1 4888 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 12 23 1 98 0 1599 1 
COMMENT             1617 1 2131 1 2295 1 2370 0 2946 0 3607 0 4124 1 4290 1 4699 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 2 1035 0 1886 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 871 1 914 1 4678 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 4 358 0 1133 1 1230 0 
COMMENT             1554 0 0 "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 5 445 0 3057 0 3099 1 
COMMENT             3252 1 4028 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 3 445 0 3057 0 3099 1 0 
COMMENT             "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 3 191 1 405 1 889 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 420 0 703 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 12 24 1 97 0 1600 1 
COMMENT             1618 1 2132 1 2296 1 2369 0 2945 0 3606 0 4125 1 4291 1 4700 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 888 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3149 0 3254 0 4262 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 391 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2242 1 2551 0 3252 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 2 3860 0 4034 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 523 0 1714 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 12 217 1 1616 1 1802 0 2784 
COMMENT             0 3449 0 3619 1 3888 0 3931 0 4049 0 4455 0 4567 1 4580 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1732 0 2287 
COMMENT             0 2372 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1185 1 3118 1 3425 0 
COMMENT             4809 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 978 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 8 337 0 777 1 1045 1 1411 
COMMENT             1 3025 1 3648 0 3935 0 4116 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 23 510 0 580 0 968 0 974 0 
COMMENT             1147 0 1507 0 1655 0 1693 0 2014 0 2225 0 3414 0 3450 0 3467 0 3725 
COMMENT             0 3771 0 3789 0 4130 0 4235 0 4247 0 4325 0 4333 0 4344 0 4419 0 0 
COMMENT             "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 18 180 0 210 0 262 0 321 0 
COMMENT             424 0 451 0 795 0 1050 0 1175 0 1197 0 1287 0 1362 0 1416 0 1913 0 
COMMENT             2080 0 4823 0 4836 0 4957 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 22 17 0 102 0 153 0 1333 0 
COMMENT             1651 0 1734 0 1739 0 1745 0 2289 0 2374 0 2467 0 2491 0 2511 0 2887 
COMMENT             0 2998 0 3101 0 3103 0 3203 0 3535 0 4028 0 4358 0 4939 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 40 12 0 15 0 40 0 146 0 
COMMENT             149 0 607 0 1166 0 1247 0 1531 0 1538 0 1589 0 1592 0 1686 0 1739 0 
COMMENT             1742 0 1745 0 1845 0 1947 0 1963 0 2245 0 2248 0 2354 0 2379 0 2382 
COMMENT             0 2480 0 2494 0 2516 0 2956 0 3065 0 3352 0 3581 0 3676 0 3703 0 
COMMENT             4042 0 4370 0 4576 0 4579 0 4644 0 4787 0 4942 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1218 0 3675 0 3695 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 38 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 602 0 710 0 808 0 985 0 1128 0 1161 0 1209 0 
COMMENT             1223 0 1533 0 1540 0 1734 0 1949 0 1958 0 2038 0 2215 0 2289 0 2293 
COMMENT             0 2297 0 2366 0 2370 0 2374 0 2384 0 2535 0 2549 0 2592 0 2968 0 
COMMENT             3981 0 4372 0 4932 0 4969 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 10 1112 1 1376 0 1391 0 
COMMENT             1520 0 1571 0 1943 0 2132 0 2708 0 3441 0 3842 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 23 511 0 581 0 969 0 975 0 
COMMENT             1148 0 1508 0 1656 0 1694 0 2015 0 2226 0 3415 0 3451 0 3468 0 3726 
COMMENT             0 3772 0 3790 0 4131 0 4236 0 4248 0 4326 0 4334 0 4345 0 4420 0 0 
COMMENT             "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 8 524 0 611 0 826 0 1433 0 
COMMENT             1715 0 2463 0 2936 0 3612 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 20 278 0 690 0 715 0 853 0 
COMMENT             1004 0 1238 0 1268 0 1658 0 1703 0 1863 0 2218 0 3066 0 3150 0 3255 
COMMENT             0 3648 0 3684 0 3762 0 3772 0 4263 0 4983 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 91 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 276 0 324 0 366 0 409 0 483 0 574 0 616 0 675 0 708 0 712 0 
COMMENT             740 0 770 0 871 0 908 0 914 0 938 0 983 0 1025 0 1058 0 1078 0 1130 
COMMENT             0 1142 0 1163 0 1178 0 1201 0 1279 0 1379 0 1427 0 1447 0 1494 0 
COMMENT             1538 0 1579 0 1612 0 1625 0 1712 0 1742 0 1884 0 1974 0 1979 0 2004 
COMMENT             0 2040 0 2245 0 2273 0 2309 0 2340 0 2354 0 2379 0 2594 0 2608 0 
COMMENT             2622 0 2703 0 2807 0 2845 0 2865 0 2966 0 2970 0 3010 0 3043 0 3062 
COMMENT             0 3116 0 3703 0 3741 0 3804 0 3814 0 3904 0 3970 0 3979 0 3983 0 
COMMENT             4009 0 4050 0 4062 0 4425 0 4454 0 4497 0 4508 0 4573 0 4652 0 4677 
COMMENT             0 4682 0 4728 0 4818 0 4916 0 4942 0 4960 0 4971 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 14 32 0 84 0 702 0 1608 0 
COMMENT             1626 0 2140 0 2304 0 2356 0 2932 0 3167 0 3593 0 4133 0 4299 0 4708 
COMMENT             0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 23 509 0 579 0 967 0 973 0 
COMMENT             1146 0 1506 0 1654 0 1692 0 2013 0 2224 0 3413 0 3449 0 3466 0 3724 
COMMENT             0 3770 0 3788 0 4129 0 4234 0 4246 0 4324 0 4332 0 4343 0 4418 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3516 0 4208 0 4227 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 1740 0 2243 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 2 300 1 3295 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 1253 1 1497 1 1947 0 
COMMENT             3952 0 4780 0 4926 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 8 162 1 1158 0 1401 0 
COMMENT             1449 0 1689 0 3428 0 4025 0 4440 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 1032 0 1686 0 2309 0 2334 0 2335 0 2345 0 2346 0 3020 0 3055 0 
COMMENT             3556 0 3907 0 4603 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 908 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 656 0 3114 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 788 0 1755 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 15 315 0 862 0 979 0 1055 
COMMENT             0 1349 0 1376 0 1391 0 1520 0 1727 0 1752 0 2007 0 2018 0 2661 0 
COMMENT             3441 0 4885 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 20 277 0 689 0 714 0 852 0 
COMMENT             1003 0 1237 0 1267 0 1657 0 1702 0 1862 0 2217 0 3065 0 3149 0 3254 
COMMENT             0 3647 0 3683 0 3761 0 3771 0 4262 0 4982 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 7 1230 0 1338 0 1965 0 
COMMENT             2487 1 2556 0 2987 0 2997 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 12 429 0 659 0 1180 0 1581 
COMMENT              0 1664 0 2238 0 2704 0 3117 0 3733 0 3955 0 3972 0 4051 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 40 10 0 13 0 38 0 144 0 
COMMENT              147 0 605 0 1164 0 1245 0 1529 0 1536 0 1587 0 1590 0 1684 0 1737 
COMMENT              0 1740 0 1743 0 1843 0 1945 0 1961 0 2243 0 2246 0 2352 0 2377 0 
COMMENT              2380 0 2478 0 2492 0 2514 0 2954 0 3063 0 3350 0 3579 0 3674 0 
COMMENT              3701 0 4040 0 4368 0 4574 0 4577 0 4642 0 4785 0 4940 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 8 344 0 764 1 1032 1 
COMMENT              1398 1 3012 1 3655 0 3942 0 4123 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3871 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 8 144 1 144 0 1076 0 
COMMENT              1740 1 1740 0 2243 0 2243 1 3701 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 25 37 0 82 0 118 0 146 0 
COMMENT              324 0 574 0 1078 0 1178 0 1494 0 1579 0 1742 0 2245 0 2273 0 2309 
COMMENT              0 2354 0 2703 0 2845 0 3116 0 3703 0 3970 0 4050 0 4508 0 4942 0 
COMMENT              4960 0 4971 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "HgaI" "gacgc" 11 16 7 46 1 407 1 2340 0 3004 
COMMENT              0 3562 0 4292 1 4870 1 0 "") 
COMMENT        #108=(CRSite (CStringList) "HhaI" "gcgc" 4 0 32 17 0 19 0 102 0 104 0 
COMMENT              989 0 1294 0 1335 0 1651 0 1734 0 1736 0 2289 0 2291 0 2374 0 2376 
COMMENT              0 2394 0 2469 0 2482 0 2491 0 2513 0 2539 0 2547 0 3000 0 3103 0 
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COMMENT              "") 
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COMMENT                              "BpmI: 4 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BpuEI: 4 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaI: 1 site") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
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COMMENT                              "BsmAI: 5 sites") 6 10 "GTCTCNNNNNN" "CAGAGNNNNNN") 
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COMMENT                              "BsmFI: 3 sites") 15 19 "GGGACNNNNNNNNNNNNNNN" 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    136^137
                     /Original_Bases="NNNNGAGTGGCCAACTCCATCACTAGGGG"
                     /vntifkey="21"
                     /label=NNNNGAGTGGCCAACTCCATCACTAGGGG
                     /note="Geneious type: Editing History Deletion"
     misc_feature    156..904
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /vntifkey="21"
                     /label=eHGT_682h
     misc_feature    911..963
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    928^929
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACATTTGCTTCTGGGATCC
                     AGNNNNNNNNNNNNNNNNNNNN"
                     NNNNNNNNNNNNN"
                     /vntifkey="21"
                     /label=AGGGCAGAGCCATCTATTGCTTACATTTGCTTCTGGGATCCAGNNNNNNN
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1004..1723
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1758..2003
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2017..2220
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2249..2389
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2481..2787
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3306..4163
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4314..4981
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1166 a      1331 c      1298 g      1191 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagac ggtctgattg caaccctcca 
      181 ggaggaaaac aaattcacaa agcagtccca ggttgccagt tctcaaagtc agcaaaccaa 
      241 atcctaacgt gcaatcttcc caggtttttc aaaagccatg ggattgatag tcagtgggga 
      301 aaggaagagg tgtcgaatcc aggcctgatg gatgggtgca gaggcaatca gacgtgtttt 
      361 cagagccact gtgctctgac acctgccccc attttccaca gagtttggct ctgaatgcgt 
      421 ccctggacca cgccctccgt ctcctttacc tggcgttcct attcttgtag aaaggaacac 
      481 agcctaatga tggatttgtt caatgtgatc attctcgctt atgtacaaaa caaaggcagg 
      541 attgatgaac tcctaaagaa gcacattcac aggcctgatc tatgcctctt accttggagc 
      601 aggcagcaag tacagcctcc aagggaatgt caacatcttt gcactttgtc aataggacct 
      661 tgattctcta aaggctgaaa ggtgttttca tgggtgaatg cttagagcca gctcatggtt 
      721 ttccaaaccc cactcaaagc ttccagatga ctctttttgc atctgtgagc ttctttcatc 
      781 cctagatatc accctgggga gagggcaggc aaaagatgtt ctctgtactt gcaaggaaga 
      841 aatggtaaag gcatgggggt cgagtggggg ctggggactc ctatgttccg gaaagagcga 
      901 ggttgagctc gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt 
      961 ctgggatcca gatctttcga agctagcgct accggtcgcc accatggtga gcaagggcga 
     1021 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 
     1081 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 
     1141 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg 
     1201 ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa 
     1261 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 
     1321 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 
     1381 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 
     1441 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt 
     1501 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa 
     1561 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc 
     1621 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 
     1681 cgccgccggg atcactctcg gcatggacga gctgtacaag taagtcgacg gcgcgccgcg 
     1741 gccgcgaatt cgatatcata atcaacctct ggattacaaa atttgtgaaa gattgactgg 
     1801 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 
     1861 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt 
     1921 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
     1981 gttgggcact gacaattccg tggctcgaga gatcttcgac tgtgccttct agttgccagc 
     2041 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 
     2101 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 
     2161 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 
     2221 agatctcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc 
     2281 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 
     2341 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 
     2461 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     2521 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     2581 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     2641 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 
     2701 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     2761 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 
     2821 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     2881 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 
     2941 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 
     3001 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 
     3061 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 
     3121 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     3181 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     3241 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     3301 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     3361 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     3421 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     3481 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     3541 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     3601 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     3661 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     3721 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     3781 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     3841 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     3901 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     3961 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     4021 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     4081 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     4141 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     4201 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     4261 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     4321 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     4381 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     4441 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 
     4501 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     4561 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     4621 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     4681 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     4741 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     4801 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     4861 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     4921 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     4981 acatgt 
//
