LOCUS       CN2408\pAAV-eHGT_600m-minBG-SYFP2-WPRE3-BGHpA\(JT)        5130 bp    DNA     circular     10-DEC-2019
SOURCE      
  ORGANISM  
COMMENT     rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
            
            clones 2,3,4 all good
            clone 1 low yield 
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13116475|
COMMENT     VNTDBDATE|-13116475|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2408 pAAV-eHGT_600m-minBG-SYFP2-WPRE3-BGHpA (JT)|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN2408 pAAV-eHGT_600m-minBG-SYFP2-WPRE3-BGHpA (JT)" 0 5130 
COMMENT       (CDBMol 0 0 1 1 1 0 0 62962520 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5130 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3069 0 3566 0 4812 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 41 0 52 0 444 0 1053 0 
COMMENT            2149 0 2479 0 2490 0 2946 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1109 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 595 0 1891 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1147 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2532 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 43 0 54 0 446 0 2481 0 
COMMENT            2492 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 41 0 52 0 444 0 2479 0 
COMMENT            2490 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 2 672 1 735 1 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3319 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 1870 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 8 1155 1 1188 1 1509 1 
COMMENT             1692 0 1842 1 2995 1 3192 1 3934 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 53 38 0 143 1 147 0 440 0 
COMMENT             1367 0 1408 0 1475 0 1514 0 1609 0 1652 0 1672 1 1825 0 1828 0 1881 
COMMENT             0 1883 1 1887 0 2076 1 2089 0 2102 0 2377 1 2386 1 2390 0 2495 1 
COMMENT             2545 1 2571 1 2584 0 2621 1 2635 1 2638 1 2666 0 2693 0 3098 0 3124 
COMMENT             0 3134 0 3173 0 3347 1 3394 0 3493 1 3602 1 3679 1 3723 0 3844 1 
COMMENT             3890 0 4081 1 4172 1 4534 0 4543 1 4678 0 4788 1 4909 1 4928 1 5055 
COMMENT             1 5083 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 3052 0 3636 0 4009 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 5 149 1 1302 0 1545 0 
COMMENT             3572 0 4584 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 1132 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 184 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 1136 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 30 12 0 183 0 219 0 874 0 
COMMENT             995 0 1052 0 1127 0 1169 0 1202 0 1274 0 1286 0 1307 0 1523 0 1571 
COMMENT             0 1682 0 1756 0 1769 0 1856 0 2523 0 2752 0 3009 0 3187 0 3206 0 
COMMENT             3885 0 3948 0 4048 0 4569 0 4826 0 4872 0 4962 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 12 1104 1 1117 0 1643 1 
COMMENT             1842 0 3599 0 3603 1 3920 0 4383 1 4384 0 4480 1 4482 0 4568 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1876 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4063 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 1805 0 2379 0 3874 0 
COMMENT             4096 0 0 "") #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 2 654 0 2539 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 485 1 2359 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 490 1 2354 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 21 21 0 532 1 889 1 1294 
COMMENT             1 1400 0 1691 0 1717 1 1720 1 1973 1 2091 1 2510 1 2532 0 2669 0 
COMMENT             3193 1 3804 1 4195 0 4498 1 4704 1 4707 1 4797 0 5129 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 14 133 0 794 0 987 0 1091 
COMMENT             0 1197 0 1449 0 1719 0 2193 0 2401 1 2824 1 2856 0 3781 1 4068 1 
COMMENT             4192 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 13 1223 0 1235 0 1283 0 
COMMENT             1364 0 1475 0 1565 0 1643 0 1685 0 1736 0 1889 0 2089 0 2812 0 4626 
COMMENT             1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 13 1223 0 1235 0 1283 0 
COMMENT             1364 0 1475 0 1565 0 1643 0 1685 0 1736 0 1889 0 2089 0 2812 0 4627 
COMMENT             1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 1974 1 3401 0 4928 0 
COMMENT             0 "") #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 8 131 0 1128 0 2174 0 2402 0 
COMMENT             2686 0 4045 0 4380 0 4633 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 3 1115 0 2155 0 2366 0 0 
COMMENT             "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 2 530 0 1770 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4198 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 1131 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1593 0 1833 0 4169 0 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 3634 0 4502 0 4743 1 
COMMENT             5041 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 7 57 0 651 0 826 0 2476 0 
COMMENT             2536 0 3316 0 3698 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 4172 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 384 0 648 0 663 1 
COMMENT             1250 1 1524 0 1539 0 1638 0 2280 0 2535 1 2571 0 3087 1 3177 0 3540 
COMMENT             1 3785 0 3980 1 5032 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 18 23 1 98 0 262 1 477 
COMMENT             0 835 0 875 1 881 1 1021 1 1743 1 1761 1 2275 1 2439 1 2514 0 3090 
COMMENT             0 3751 0 4268 1 4434 1 4843 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 3 809 1 1179 0 2030 1 0 
COMMENT             "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 2 1058 1 4822 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 4 452 0 1277 1 1374 0 
COMMENT             1698 0 0 "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 5 579 0 3201 0 3243 1 
COMMENT             3396 1 4172 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3201 0 3243 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 18 24 1 97 0 263 1 476 
COMMENT             0 834 0 876 1 882 1 1022 1 1744 1 1762 1 2276 1 2440 1 2513 0 3089 
COMMENT             0 3750 0 4269 1 4435 1 4844 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 4 1035 0 3293 0 3398 0 
COMMENT             4406 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 2 672 1 735 1 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2386 1 2695 0 3396 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 2 4004 0 4178 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 1858 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 14 348 1 939 0 968 1 1760 1 
COMMENT             1946 0 2928 0 3593 0 3763 1 4032 0 4075 0 4193 0 4599 0 4711 1 4724 
COMMENT             1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1876 0 2431 
COMMENT             0 2516 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1329 1 3262 1 3569 0 
COMMENT             4953 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 1122 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 10 376 1 597 0 675 0 975 
COMMENT             1 1189 1 1555 1 3169 1 3792 0 4079 0 4260 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 23 571 0 625 0 1112 0 1118 
COMMENT             0 1291 0 1651 0 1799 0 1837 0 2158 0 2369 0 3558 0 3594 0 3611 0 
COMMENT             3869 0 3915 0 3933 0 4274 0 4379 0 4391 0 4469 0 4477 0 4488 0 4563 
COMMENT             0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 14 707 0 853 0 899 0 1194 
COMMENT             0 1319 0 1341 0 1431 0 1506 0 1560 0 2057 0 2224 0 4967 0 4980 0 
COMMENT             5101 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 22 17 0 102 0 153 0 1477 0 
COMMENT             1795 0 1878 0 1883 0 1889 0 2433 0 2518 0 2611 0 2635 0 2655 0 3031 
COMMENT             0 3142 0 3245 0 3247 0 3347 0 3679 0 4172 0 4502 0 5083 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 41 12 0 15 0 40 0 146 0 
COMMENT             149 0 548 0 905 0 1310 0 1391 0 1675 0 1682 0 1733 0 1736 0 1830 0 
COMMENT             1883 0 1886 0 1889 0 1989 0 2091 0 2107 0 2389 0 2392 0 2498 0 2523 
COMMENT             0 2526 0 2624 0 2638 0 2660 0 3100 0 3209 0 3496 0 3725 0 3820 0 
COMMENT             3847 0 4186 0 4514 0 4720 0 4723 0 4788 0 4931 0 5086 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1362 0 3819 0 3839 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 37 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 830 0 876 0 1129 0 1272 0 1305 0 1353 0 1367 0 
COMMENT             1677 0 1684 0 1878 0 2093 0 2102 0 2182 0 2359 0 2433 0 2437 0 2441 
COMMENT             0 2510 0 2514 0 2518 0 2528 0 2679 0 2693 0 2736 0 3112 0 4125 0 
COMMENT             4516 0 5076 0 5113 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 10 1256 1 1520 0 1535 0 
COMMENT             1664 0 1715 0 2087 0 2276 0 2852 0 3585 0 3986 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 23 572 0 626 0 1113 0 1119 0 
COMMENT             1292 0 1652 0 1800 0 1838 0 2159 0 2370 0 3559 0 3595 0 3612 0 3870 
COMMENT             0 3916 0 3934 0 4275 0 4380 0 4392 0 4470 0 4478 0 4489 0 4564 0 0 
COMMENT             "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 7 778 0 1012 0 1577 0 1859 
COMMENT             0 2607 0 3080 0 3756 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 19 837 0 987 0 1036 0 1148 
COMMENT             0 1382 0 1412 0 1802 0 1847 0 2007 0 2362 0 3210 0 3294 0 3399 0 
COMMENT             3792 0 3828 0 3906 0 3916 0 4407 0 5127 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 97 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 175 0 183 0 219 0 277 0 359 0 462 0 513 0 529 0 534 0 704 0 
COMMENT             722 0 793 0 832 0 841 0 874 0 902 0 951 0 995 0 1029 0 1052 0 1058 
COMMENT             0 1082 0 1127 0 1169 0 1202 0 1222 0 1274 0 1286 0 1307 0 1322 0 
COMMENT             1345 0 1423 0 1523 0 1571 0 1591 0 1638 0 1682 0 1723 0 1756 0 1769 
COMMENT             0 1856 0 1886 0 2028 0 2118 0 2123 0 2148 0 2184 0 2389 0 2417 0 
COMMENT             2453 0 2484 0 2498 0 2523 0 2738 0 2752 0 2766 0 2847 0 2951 0 2989 
COMMENT             0 3009 0 3110 0 3114 0 3154 0 3187 0 3206 0 3260 0 3847 0 3885 0 
COMMENT             3948 0 3958 0 4048 0 4114 0 4123 0 4127 0 4153 0 4194 0 4206 0 4569 
COMMENT             0 4598 0 4641 0 4652 0 4717 0 4796 0 4821 0 4826 0 4872 0 4962 0 
COMMENT             5060 0 5086 0 5104 0 5115 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 22 32 0 84 0 271 0 322 0 
COMMENT             463 0 530 0 821 0 884 0 890 0 996 0 1030 0 1752 0 1770 0 2284 0 
COMMENT             2448 0 2500 0 3076 0 3311 0 3737 0 4277 0 4443 0 4852 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 23 570 0 624 0 1111 0 1117 0 
COMMENT             1290 0 1650 0 1798 0 1836 0 2157 0 2368 0 3557 0 3593 0 3610 0 3868 
COMMENT             0 3914 0 3932 0 4273 0 4378 0 4390 0 4468 0 4476 0 4487 0 4562 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3660 0 4352 0 4371 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 1884 0 2387 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 3 328 1 918 0 3439 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 1397 1 1641 1 2091 0 
COMMENT             4096 0 4924 0 5070 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 8 149 1 1302 0 1545 0 
COMMENT             1593 0 1833 0 3572 0 4169 0 4584 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 20 40 0 41 0 51 0 52 0 77 
COMMENT             0 443 0 444 0 537 0 1176 0 1830 0 2453 0 2478 0 2479 0 2489 0 2490 
COMMENT             0 3164 0 3199 0 3700 0 4051 0 4747 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 1052 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 512 0 3258 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1899 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 13 1123 0 1199 0 1493 0 
COMMENT             1520 0 1535 0 1664 0 1871 0 1896 0 2151 0 2162 0 2805 0 3585 0 5029 
COMMENT             0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 19 836 0 986 0 1035 0 1147 0 
COMMENT             1381 0 1411 0 1801 0 1846 0 2006 0 2361 0 3209 0 3293 0 3398 0 3791 
COMMENT             0 3827 0 3905 0 3915 0 4406 0 5126 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 7 1374 0 1482 0 2109 0 
COMMENT             2631 1 2700 0 3131 0 3141 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 14 515 0 706 0 795 0 834 0 
COMMENT              1324 0 1725 0 1808 0 2382 0 2848 0 3261 0 3877 0 4099 0 4116 0 
COMMENT              4195 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 41 10 0 13 0 38 0 144 0 
COMMENT              147 0 546 0 903 0 1308 0 1389 0 1673 0 1680 0 1731 0 1734 0 1828 0 
COMMENT              1881 0 1884 0 1887 0 1987 0 2089 0 2105 0 2387 0 2390 0 2496 0 
COMMENT              2521 0 2524 0 2622 0 2636 0 2658 0 3098 0 3207 0 3494 0 3723 0 
COMMENT              3818 0 3845 0 4184 0 4512 0 4718 0 4721 0 4786 0 4929 0 5084 0 0 
COMMENT              "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 10 363 1 604 0 682 0 962 
COMMENT              1 1176 1 1542 1 3156 1 3799 0 4086 0 4267 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 4015 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 8 144 1 144 0 1220 0 
COMMENT              1884 1 1884 0 2387 0 2387 1 3845 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 28 37 0 82 0 118 0 146 0 
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COMMENT                             "GCAGTGNNN" "CGTCACNNN") 
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COMMENT                              "EagI: 3 sites") 1 5 "CGGCCG" "GCCGGC") 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
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COMMENT                             "CTGRAGNNNNNNNNNNNNNNNNN" "GACYTCNNNNNNNNNNNNNNNNN") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    2393..2533
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2625..2931
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3450..4307
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4458..5125
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    2161..2364
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1902..2147
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1902..2061
                     /vntifkey="21"
                     /label=gamma
     misc_feature    2067..2147
                     /vntifkey="21"
                     /label=alpha
     misc_feature    1055..1107
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    1148..1867
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    156..1048
                     /vntifkey="21"
                     /label=eHGT_600m
BASE COUNT     1188 a      1390 c      1340 g      1212 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaacag gacttcaggg aaggccacgg 
      181 agcttaagca agaacttgcc caaagtttag aaagaaagct ccaagttaca gaaccaagtg 
      241 cccgacccca gagtctttct ttgtctgacc tgagggcttt ctgttaaaat ccttatacag 
      301 cagataagta agtcagcagt cttagaggtg ggaagaggtc tgtctgccag tcatctggct 
      361 ccctgcaaac acaggcatcc ccacccctga acaattttct tcataggaaa gttgtgaggg 
      421 aagtcacagg tgcagacacc gccccggggg gggagggggg gctcagagtt gttccttcat 
      481 tcagtcacca attcagtctt ccagaatcaa ggccctattg acagagggct aagccaccgg 
      541 ggcgctgcct tcaaggtaag aggcatagat cagtctcctg aatgtagagg gatgaattcc 
      601 ctccttcaca taagtgaaag cgatcatagg gaacggaggc atcattaggg cgccctcctt 
      661 ctgacaggga tgcagacagg caggtgaggg tggaggggtg tggcccaggg cacattcaga 
      721 gccaaactct gttgagaaca aggcaggtgt ttctgaaaac ctctctgatt gcacccgtac 
      781 cctcccatca ggcccgaatc gcacacctct ctgcctctcc tcagacgccg gcccacatgg 
      841 cttttgaaaa cctggggaga ttataccttc cagcttgctt tgctgagtct gaggaacctg 
      901 gctgcctttt ctcttctgtt aactgcaatc aaactggttt tgagcaccag ccccaagatg 
      961 tctttgccag tccacatcca tcttccatga tgagctaagg ttccctattt gtacccttac 
     1021 tttctgggct gagtcatgac atctgggaga gctcgggctg ggcataaaag tcagggcaga 
     1081 gccatctatt gcttacattt gcttctggga tccagatctt tcgaagctag cgctaccggt 
     1141 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 
     1201 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 
     1261 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg 
     1321 gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccgct accccgacca 
     1381 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 
     1441 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 
     1501 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 
     1561 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca 
     1621 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca 
     1681 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 
     1741 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca 
     1801 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 
     1861 caagtaagtc gacggcgcgc cgcggccgcg aattcgatat cataatcaac ctctggatta 
     1921 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     1981 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     2041 ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc 
     2101 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggctc gagagatctt 
     2161 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
     2221 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
     2281 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
     2341 attgggaaga caatagcagg catgagatct cacgtgcgga ccgagcggcc gcaggaaccc 
     2401 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga 
     2461 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc 
     2521 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 
     2581 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 
     2641 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     2701 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     2761 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     2821 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     2881 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     2941 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     3001 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 
     3061 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 
     3121 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 
     3181 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 
     3241 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 
     3301 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 
     3361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 
     3421 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 
     3481 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 
     3541 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 
     3601 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 
     3661 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 
     3721 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 
     3781 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 
     3841 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 
     3901 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 
     3961 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 
     4021 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 
     4081 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 
     4141 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 
     4201 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 
     4261 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 
     4321 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     4381 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     4441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     4501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     4561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     4621 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     4681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     4741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     4801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     4861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     4921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     4981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     5041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     5101 tggccttttg ctggcctttt gctcacatgt 
//
