LOCUS       CN4134_pAAV-3xcore2_eHGT_452h-minBglobin-jGCaMP8m-WPRE3-BGHpA        5446 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...ml-el4vzfj
VERSION     urn.local...ml-el4vzfj
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA clone #8-1 correct , 8-2
                       backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..839
                     /created_by="ximena.opitzaraya"
                     /modified_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=3xcore2_eHGT_452h
     misc_feature    156..383
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=core2
     misc_feature    846..898
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     source          <922..>2195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    922..2195
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=jGCaMP8m
     CDS             930..947
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /vntifkey="4"
                     /label=6xHis
     misc_feature    2224..2469
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2483..2686
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2709..2849
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2941..3247
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3766..4623
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4774..5441
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1354 a      1441 c      1368 g      1283 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgaa aaatacctag tcttgaactt 
      181 tggagttcca aattccacat gatcgacttc ctcaaggctt ttcacttgac gaacaaattg 
      241 catgccaaga aatcgtcaaa attctatcaa gcttaattat gcaatttctg tctataaatg 
      301 acatacctcc tgtaccctat gacacccacc tatgcactaa taagatgtga cagctgcatc 
      361 accaacaaag cctcccccgc ctggtgaaaa atacctagtc ttgaactttg gagttccaaa 
      421 ttccacatga tcgacttcct caaggctttt cacttgacga acaaattgca tgccaagaaa 
      481 tcgtcaaaat tctatcaagc ttaattatgc aatttctgtc tataaatgac atacctcctg 
      541 taccctatga cacccaccta tgcactaata agatgtgaca gctgcatcac caacaaagcc 
      601 tcccccgcct ggtgaaaaat acctagtctt gaactttgga gttccaaatt ccacatgatc 
      661 gacttcctca aggcttttca cttgacgaac aaattgcatg ccaagaaatc gtcaaaattc 
      721 tatcaagctt aattatgcaa tttctgtcta taaatgacat acctcctgta ccctatgaca 
      781 cccacctatg cactaataag atgtgacagc tgcatcacca acaaagcctc ccccgcctgg 
      841 agctcgggct gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg 
      901 atccagatct ttcgaagcta gccaccatgc atcatcacca tcatcacacg cgtcgcaaga 
      961 agaccttcaa ggaggtggcc accgccgtga agatcatcgg ccgcctgatg ggcctgaaga 
     1021 tcaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac ttccacatcc 
     1081 gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca 
     1141 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtggag tccaaacttt 
     1201 cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 
     1261 ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagca 
     1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 
     1381 acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga 
     1441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 
     1501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 
     1561 tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg 
     1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 
     1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 
     1741 acaacctgcc ggaccaactg actgaagagc agatcgcaga atacaaagag gctttctccc 
     1801 tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg gtgatgcggt 
     1861 ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa gtagatgccg 
     1921 acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga aaaatgaaat 
     1981 acagggacac ggaagaagaa attagagaag cgttcggtgt gtttgataag gatggcaatg 
     2041 gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag aagttaacag 
     2101 atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt caggtaaact 
     2161 acgaagagtt tgtacaaatg atgacagcga agtaaggcgc gccgcggccg cgaattcgat 
     2221 atcataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 
     2281 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 
     2341 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg 
     2401 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 
     2461 attccgtggc tcgagagatc ttcgactgtg ccttctagtt gccagccatc tgttgtttgc 
     2521 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 
     2581 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 
     2641 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgcacg tgcggaccga 
     2701 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct 
     2761 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt 
     2821 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca 
     2881 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc 
     2941 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     3001 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     3061 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 
     3121 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     3181 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     3241 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt 
     3301 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 
     3361 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag 
     3421 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 
     3481 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 
     3541 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 
     3601 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 
     3661 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     3721 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     3781 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     3841 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     3901 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     3961 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     4021 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     4081 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     4141 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     4201 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     4261 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     4321 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     4381 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     4441 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     4501 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     4561 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     4621 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     4681 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     4741 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     4801 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     4861 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     4921 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 
     4981 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     5041 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     5101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     5161 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     5221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     5281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     5341 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     5401 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt 
//
