LOCUS       CN2610_rAAV-eHGT_780m-minBglobin-SYFP2-WPRE3-BGHpA        4771 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...1p1-c05p9wv
VERSION     urn.local...1p1-c05p9wv
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2610_rAAV-eHGT_780m-minBglobin-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 7 0 594 0 2173 0 0 "") 
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COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2120 0 2131 0 0 
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COMMENT        #13=(CFSignal (CObList) "SYFP2" 21 0 0 789 1508 0 (CStringList) 
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COMMENT                          "Gibson/Homology Cloning ligation product." 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/urn.local...1p1-c05p9wv\">urn.local...1p1-c05p9wv</a>" 
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COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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COMMENT          #244=(CWidgetStyle "Annotation" 0 0 1 
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COMMENT                             #336=(CScratch 
COMMENT                                   (CWidget 6 (1 #1# 6) 1 2 0 0 #223# 70200240 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.92091 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #337=(CLabel (CWidget 0 (0 0) 1 2 0 0 #215# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (1790)" "@N (@S)" 5 11.598 
COMMENT                                   -13.9386 0 -10 4.22101 0.949103 #336#) 
COMMENT                             #338=(CScratch 
COMMENT                                   (CWidget 7 (1 #1# 7) 1 2 0 0 #223# 70199760 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.487 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #339=(CLabel (CWidget 0 (0 0) 1 2 0 0 #215# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (2120)" "@N (@S)" 5 7.51512 
COMMENT                                   -20.908 0 -10 4.22101 0.949103 #338#) 
COMMENT                             #340=(CScratch 
COMMENT                                   (CWidget 8 (1 #1# 8) 1 2 0 0 #223# 70206960 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.47254 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #341=(CLabel (CWidget 0 (0 0) 1 2 0 0 #215# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (2131)" "@N (@S)" 5 7.34614 
COMMENT                                   -26.1349 0 -10 4.14608 0.949103 #340#) 
COMMENT                             #342=(CScratch 
COMMENT                                   (CWidget 9 (1 #1# 9) 1 2 0 0 #223# 101461416 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.87296 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #343=(CLabel (CWidget 0 (0 0) 1 2 0 0 #215# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (2587)" "@N (@S)" 5 -6.2443 
COMMENT                                   -12.1963 0 -10 4.29594 0.949103 #342#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #344=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1629633908 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #345=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -945 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 72139408 
COMMENT       (CStringList) 72144968 72144688 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    136^137
                     /Original_Bases="NTNNGACATGNNANNNGTNNNNNCGTTGNGNCACCNNTGGT
                     TANCCCNGNNTACNNTNACCNNNNNNNNNNNNNNNNNNNNGAGTGGCCAACTCCATCA
                     CTAGGGG"
                     NTACNNTNACCNNNNNNNNNNNNNNNNNNNNGAGTGGCCAACTCCATCACTAGGGG"
                     /vntifkey="21"
                     /label=NTNNGACATGNNANNNGTNNNNNCGTTGNGNCACCNNTGGTTANCCCNGN
                     /note="Geneious type: Editing History Deletion"
     misc_feature    156..689
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /vntifkey="21"
                     /label=eHGT_780m
     misc_feature    696..748
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    713^714
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACATTTGCTTCNGGGANCC
                     AGNNNTNNNNNNNNNNNNN"
                     NNNNNNNNNN"
                     /vntifkey="21"
                     /label=AGGGCAGAGCCATCTATTGCTTACATTTGCTTCNGGGANCCAGNNNTNNN
                     /note="Geneious type: Editing History Deletion"
     misc_feature    789..1508
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1543..1788
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1802..2005
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2034..2174
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2266..2572
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3091..3948
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4099..4766
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1118 a      1292 c      1238 g      1123 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgttgttt cacaaactac aggttcactg 
      181 gagcacagcc atgccaacat ggcatctaag gctggtatga cctcgagacc cctccagcac 
      241 tccttcgttc agccatagaa caaggagaca cagataaagt ctggagccgg gcccaggtac 
      301 ctgaggctca gagatgagcc cgcagcgaag ctgacaactg actcattcct ccgacactct 
      361 cccaccctcc cagctaagga ggtgtctgat gtgaacatgt tctgcctctt tgttgcctgc 
      421 tgttgggggc gagggctttg ggtggatttt gaagagcaat tatgcttatt actggttagg 
      481 atttctctgt ttgctgagtg cattaacttt tagcgcttcc catcactcat tcttcttcac 
      541 accctcttgt cagttacaaa tgtgcaggtg gagagccacg aaaaaaccct gcagcaacgg 
      601 caacagaaat cacagcaaca acaacaaatg aacagaaagg atagaaagca gatactcaaa 
      661 gatggagaaa aagatactcc cgagaggctg agctcgggct gggcataaaa gtcagggcag 
      721 agccatctat tgcttacatt tgcttctggg atccagatct ttcgaagcta gcgctaccgg 
      781 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
      841 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 
      901 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct 
      961 ggcccaccct cgtgaccacc ctgggctacg gcgtgcagtg cttcgcccgc taccccgacc 
     1021 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 
     1081 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     1141 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     1201 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc 
     1261 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc 
     1321 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     1381 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc 
     1441 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     1501 acaagtaagt cgacggcgcg ccgcggccgc gaattcgata tcataatcaa cctctggatt 
     1561 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
     1621 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
     1681 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg 
     1741 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggct cgagagatct 
     1801 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 
     1861 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 
     1921 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 
     1981 gattgggaag acaatagcag gcatgagatc tcacgtgcgg accgagcggc cgcaggaacc 
     2041 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 
     2101 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 
     2161 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 
     2221 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg 
     2281 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     2341 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     2401 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     2461 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     2521 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     2581 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 
     2641 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 
     2701 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 
     2761 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 
     2821 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 
     2881 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 
     2941 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     3001 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     3061 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     3121 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     3181 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     3241 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     3301 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     3361 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     3421 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     3481 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     3541 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     3601 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     3661 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     3721 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     3781 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 
     3841 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     3901 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     3961 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     4021 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     4081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     4141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     4201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     4261 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     4321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     4381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     4441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     4501 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     4561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     4621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     4681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     4741 ctggcctttt gctggccttt tgctcacatg t 
//
