LOCUS       BB115_pRS426_csr       10742 bp DNA     circular SYN 12-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Dunlap-Loros
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    1948..2940
                     /label=N crassa csr-1 5' homology
     primer_bind     1948..1968
                     /label=Full csr f
     misc_feature    2971..2993
                     /label=1 kb f primer
     misc_feature    3180..3198
                     /label=800 bp f primer
     misc_feature    3395..3414
                     /label=600 bp f primer
     misc_feature    3603..3624
                     /label=400 bp f primer
     misc_feature    3796..3814
                     /label=200 bp f primer
     misc_feature    3977..4001
                     /label=r primer
     mRNA            join(4001..4265,4332..4783,4855..5209)
                     /locus_tag="NCU04288"
                     /product="Poly(A)+ RNA export protein"
                     /label=NCU04288 mRNA
                     /db_xref="GI:758997004"
                     /db_xref="GeneID:3877232"
     gene            4001..5209
                     /locus_tag="NCU04288"
                     /label=NCU04288
                     /db_xref="GeneID:3877232"
     CDS             join(4002..4265,4332..4783,4855..5209)
                     /codon_start=1
                     /locus_tag="NCU04288"
                     /product="Poly(A)+ RNA export protein"
                     /label=NCU04288
                     /db_xref="GI:85101044"
                     /db_xref="GeneID:3877232"
                     /protein_id="XP_961078.1"
                     /translation="MAGLFGSAAASASNTLGDLKQDVELGQPPEDSISDLAFNPNPADQ
                     KDFLAVASWDKKTRIYEILSNGQGQGQAMIEHDAPVFSCDFFKDGTKVISAGADKAAKV
                     LDLATGQSMQVAAHDMPIKCVRYFEANGTPMAVTGGWDKQIKYWDFRSANPAATVQAQE
                     RVYTMDVRDNLLVVGTADRYINVINLKDPGKFYKTMQSPLKWQTRVVSCFNDSQGFAIG
                     SIEGRCAIQYVEDKDSASNFSFKCHRDPAQGNTTAVHAVNDISFHPQHGTFSTAGSDGT
                     FHFWDKDAKHRLKGYPNVGGSITSTTFNKTGSIFAYAISYDWSKGYQGNSPTYPTKVML
                     HPVQQDECKPRPSVKKR"
     misc_feature    5210..5236
                     /label=linker
     CDS             5237..5947
                     /codon_start=1
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    5973..7184
                     /label=N crassa csr-1 3' homology
     primer_bind     complement(7163..7184)
                     /label=Full csr r
     protein_bind    7203..7219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7227..7257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(7581..8169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8340..9200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9201..9305)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9332..10674
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgct ggagaagctc attccacccg aaatgcccgt
     1981 gaacttgccc ttgtttgcgc gggccttctt gcgctctgct cggatacgct cgacatcgct
     2041 gaggagctcg gcgagttcct tggcgcggtt gcggacgttg ataccctggt ctttgccgtt
     2101 ctggtcgata aaatggaact ggcgcagcat cttgaggagg gtaagatgcg accgagcatc
     2161 gtccaccact cgttcggaac cgtgcttaat caggtactct agtagttgaa gggccttgta
     2221 gatctggcgc cattcttcgg cggacttttc tgtgaaacgg cggtaaatca tgggcatgat
     2281 ttcgttgagt gtttggctaa gaacaaggag ggaaacgcaa gttagccgcg ttcgtataca
     2341 agcgagcagt tctcgagaac gtactagttg aaggtgccat cggcgatctc ttgcatcaaa
     2401 gtcgacgatg caccccaagg ttcattgttg gtggcctcgc gcacctgggt tcgctgtcag
     2461 ctcccgttcc atggtctctt gtctgggatc aagtatatgg cgaagggcgc aagagaatgg
     2521 tgccagtgtg gcgcgtggat acgggcatac cttggactcc atctcggtat agttcatgac
     2581 agctggttgg agccattgtc agcaccctga acacgaaatc atgagggagt cttggttctg
     2641 gactaggtgg tcacctacca ttttgaacct ttcgtacgcc agccttgatg tcgtagagtg
     2701 tcaggttgga cacggtatcc ttgaggctat tgaaatccat tgcgtaaacg tgtctatcgc
     2761 cggagttgct gggaattgtt ctgatgggct aaggtaattc ttctctagat ccaatctgag
     2821 cctcgcggtg atgattgcca gacgggcggg atgctgtggc gacttggtgg tttttgttcg
     2881 ggctgatcgc tttcagggcg acgtttcacg gtcttgtcgg gatcagatgg acattgaggt
     2941 gcggaattat acgatttagg tgactgcagg ccatacagat ctccctagac gccgggccaa
     3001 tccgttcctt gcaccttctc tcacacatgc aggttttgga atgtggcttc atcgtgtcca
     3061 gaactgggcg cattggggcc aggctcagtg tcagtgtagg gatcgatgta catatggcag
     3121 ttgacagcga gagagccagg tgtcacaggc agtccgtcag tggccgcggt ggtggccacg
     3181 cactttctgc aggagccgtc tggggtgcgt gcagctggcg gccgcttccg ggcatacggc
     3241 ttgtgtaacg agggtgttga ccctcggctt ggcgcttgaa cttggcgctg cggtcccccc
     3301 acagttgacc acaatccccg gccaatcgaa cgacggcaac aagggcgcac tagccagtga
     3361 acgcctgggg atagtgggtg gtcaaagaga attgggcaaa cgagacgaca atggagggtg
     3421 aaccgggaat aataaacagc tggtgagacc acccatcaaa cgaatcaccc caccatggga
     3481 tgccctcaga tgccctccat gccaccataa ggaatgatct tacggcttgc cgaagttaac
     3541 aggcctgctt ttgcatatga acgagagcct ggtgttgcat tccacattgg ctatctacag
     3601 tacctaccca acctacacac cctgatgcga aagggacaga aggggagaag gatacataca
     3661 tatacctgac tcgtgcccat caacaacaga gaaagggtga tcgctgaaca catcagagca
     3721 tctcttcccc cctggcgact aaaagtgctc ccaatctcgt cttacatctg atacgttgtt
     3781 caaactccgc aacccgatct gcacactgcc ctgccttggc ccttcttgga gtctccgctt
     3841 ccccacgcca tctcacgctc gaggactccg ctgctaggta tgagctaggc ctgccgtcga
     3901 caggatccac cagacgtcaa caataaccca tcttggaaat acataagaag acgtcacttc
     3961 cctccctttt cgaaaggaag agatcatcat cagcatccat catggctggt ctttttggtt
     4021 ccgccgccgc atccgcgagc aacaccctcg gcgatcttaa gcaggatgtc gagctcggcc
     4081 aaccccccga ggacagcatc agcgaccttg ccttcaaccc caaccccgcc gaccagaagg
     4141 acttcctcgc cgtagccagc tgggacaaga agacacgcat ttacgagatc ttgagtaatg
     4201 gtcaaggtca gggacaggcc atgattgagc atgatgcccc cgttttctct tgcgatttct
     4261 tcaaggtatg tttgcgtcga ttggcctggc aggtagcccg acaaaacact gacatgcact
     4321 tctcccaaca ggacggcaca aaggtcattt ccgccggcgc cgacaaggcc gccaaggttc
     4381 tcgatttggc caccggtcag tcgatgcagg tcgctgctca cgatatgccc atcaagtgcg
     4441 ttcgctactt cgaggccaat ggcacgccca tggcggtgac aggtggctgg gacaagcaga
     4501 tcaagtactg ggatttccgc tccgcgaacc cggccgccac ggtgcaggcc caggagcgcg
     4561 tttataccat ggatgtgcgc gacaacttgc tcgtggtcgg aaccgctgat cgctatatca
     4621 acgtgatcaa cctgaaggac cccggcaagt tctacaagac aatgcagagc cctcttaaat
     4681 ggcagactcg tgtggtcagc tgcttcaacg attctcaggg ctttgctatc ggctcgatcg
     4741 agggtagatg cgccattcaa tatgtggagg acaaggattc tgcgtacgcc ttcacctcct
     4801 ccccctttac tatcccatct gccgaatacg ctgactaact gacttgtttt ccagctccaa
     4861 cttctctttc aaatgccacc gcgaccccgc ccaaggcaac accacggccg tccacgctgt
     4921 caacgacatc tccttccacc cccaacacgg caccttcagc acagccggta gcgacggcac
     4981 cttccacttc tgggacaagg acgccaagca ccgtctcaag ggttacccca acgtcggcgg
     5041 cagcatcacc tcgacaacgt tcaacaagac cggcagcatc tttgcctacg ccattagtta
     5101 tgactggagc aaggggtacc aaggcaactc gcccacgtac ccgaccaagg tcatgctcca
     5161 cccagtacag caggacgagt gcaagccccg tccgagcgtt aagaagaggg ctggtattgg
     5221 tttggctatt gctggcatgg tctccaaggg cgaggaggac aacatggcct ccctccccgc
     5281 cacccacgag ctccacatct tcggctccat caacggcgtc gacttcgaca tggtcggcca
     5341 gggcaccggc aaccccaacg acggctacga ggagctcaac ctcaagtcca ccaagggcga
     5401 cctccagttc tccccctgga tcctcgtccc ccacatcggc tacggcttcc accagtacct
     5461 cccctacccc gacggcatgt cccccttcca ggccgccatg gtcgacggct ccggctacca
     5521 ggtccaccgc accatgcagt tcgaggacgg cgcctccctc accgtcaact accgctacac
     5581 ctacgagggc tcccacatca agggcgaggc ccaggtcaag ggcaccggct tccccgccga
     5641 cggccccgtc atgaccaact ccctcaccgc cgccgactgg tgccgctcca agaagaccta
     5701 ccccaacgac aagaccatca tctccacctt caagtggtcc tacaccaccg gcaacggcaa
     5761 gcgctaccgc tccaccgccc gcaccaccta caccttcgcc aagcccatgg ccgccaacta
     5821 cctcaagaac cagcccatgt acgtcttccg caagaccgag ctcaagcact ccaagaccga
     5881 gctcaacttc aaggagtggc agaaggcctt caccgacgtc atgggcatgg acgagctcta
     5941 caagtgacta gaggatccga cactatagaa tacctagaca gtttgcaggt catgtgaatg
     6001 ttatcgccaa cacattgaat gctaaacaga ttacctccaa cgcctagatg aaaccaaatt
     6061 acccttgcct tctttttttt tacgccgtta atgcagttgt gatagagacc atgcaacgtc
     6121 ttctaaacgg agacacaaac acattcgagt cgatcagatc gaaatgactt gttcctgtac
     6181 ctcgtcacca tccagccacc gtctgaaata gacgcagttg ctggggctga caggtccgcc
     6241 ttcctcgcac atatcataga cagggcaccg cccacaaggc gcctccataa ggccacttgc
     6301 actcggctca actcccataa atgaggtaac aatgcctcct ttggttggat catggtcgag
     6361 gcgtcgttga ttgcacgtcc tcggatcctg tttggggatg cgcgtcacac ggtacccctt
     6421 gcgcccattg accttgacag gctctactaa gccatcgtag tatagcacat ccaccaactg
     6481 ctggatatcc gcctcgctca acgtcgtatt attcgtgatg ccggcgtggt ggatgaactt
     6541 tgcaatgtcc gacacagtcg ggtacgactt gtaaccggcc gggagaggca gcaattgcgg
     6601 cttgccgggc ctggtcttct tggccggtgc cggtgccgcc gtgtcgtcgt tggaaatctc
     6661 gcccgcgctg cgcttcgtcc ccttggaaat atcctccgag tggggaatgg tcccctgaac
     6721 cacgcccttc ttgggattct tttcacgctg ctgtgctcct cctccttgtg ctcctactcc
     6781 tggtactcca gcaccgccgc cgtgggaagt atatgtcgat ttctgcttga taaagtcaaa
     6841 cacaatttcc tgtagctcgt tgatgaaggc cgtgtcgagc tcgccctcag taaaccaggg
     6901 gccacctgtg gcgcgatcgg agggacgtag gttcgccttg atgtacatct tcttgttggg
     6961 gtgctcgacg ttcttcatgc tggcgatgta gcccttttgc tcgagatact tgatgcaatg
     7021 cttcataagc gagtcggcca tgttgagctt gttcttgatg gtccggttcc agatgccgtc
     7081 ggccccggct tcgtcgacca tggcgtatac catcattatt tcggagttgg ggagggaggt
     7141 gtaccttttg ggaggaaggg gaggttagtt ggtgtgctcg gttggctgtt tcctgtgtga
     7201 aattgttatc cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc
     7261 tggggtgcct aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9301 ccgcgcacat ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga
     9361 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     9421 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     9481 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     9541 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     9601 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     9661 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     9721 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     9781 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     9841 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     9901 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     9961 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
    10021 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
    10081 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
    10141 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
    10201 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
    10261 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
    10321 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
    10381 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
    10441 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
    10501 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
    10561 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
    10621 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta
    10681 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10741 tc
//
