LOCUS       pRS426_csr-1_hH1       10614 bp DNA     circular SYN 24-MAR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Oegema Desai Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 13, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /note="color: #ff6666"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ff6666"
     misc_feature    1948..2940
                     /label=N crassa csr-1 5' homology
                     /note="color: #3366ff"
     misc_feature    2971..3892
                     /label=N crassa ccg-1 promoter
                     /note="Neurospora crassa ccg-1 (grg-1) promoter"
                     /note="color: #ffcc00; direction: RIGHT"
     CDS             join(3914..4036,4091..4117,4298..4320,4486..4616,
                     4684..5087)
                     /codon_start=1
                     /label=hH1 CDS
                     /note="This feature has 5 segments:
                        1: 3914 .. 4036 / #ff6600 / Exon 1
                        2: 4091 .. 4117 / #ff6600 / Exon 2
                        3: 4298 .. 4320 / #ff6600 / Exon 3
                        4: 4486 .. 4616 / #ff6600 / Exon 4
                        5: 4684 .. 5087 / #ff6600 / Exon 5"
                     /translation="MPPKKTETKAADASAAAAPAPAAAPTSAPKTKSPSTHASYLDMIT
                     DAIVALKDRAGSSRQALKKYVRANNTLGNVTDNMFDSLFNKALKNGVDKGVFEQPKGPS
                     GGTKLAKKVAKPAPKKAAPKKETKEKKPAAAKKEGAAKKETKEKKAPAAKKAAAPKKAA
                     APKKEVKEKKAAAPKKKAAAPAVADKETVLTKTKSGRVAKSTAKPAAAKKAAAPKKAAA
                     SKKAEKAEPAAEKA"
     CDS             5097..5774
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /note="color: #ff0000"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             5775..5816
                     /codon_start=1
                     /label=V5
                     /note="color: #ff00ff"
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5845..7056
                     /label=N crassa csr-1 3' homology
                     /note="color: #3366ff"
     protein_bind    7075..7091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7099..7129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7099 .. 7105 / #ffffff / -10
                        2: 7106 .. 7123 / #ffffff
                        3: 7124 .. 7129 / #ffffff / -35"
     rep_origin      complement(7453..8041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ff6666"
     CDS             complement(8212..9072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8212 .. 9003 / #993366
                        2: 9004 .. 9072 / #993366 / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9073..9177)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      9204..10546
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
                     /note="color: #ff6666"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgct ggagaagctc attccacccg aaatgcccgt
     1981 gaacttgccc ttgtttgcgc gggccttctt gcgctctgct cggatacgct cgacatcgct
     2041 gaggagctcg gcgagttcct tggcgcggtt gcggacgttg ataccctggt ctttgccgtt
     2101 ctggtcgata aaatggaact ggcgcagcat cttgaggagg gtaagatgcg accgagcatc
     2161 gtccaccact cgttcggaac cgtgcttaat caggtactct agtagttgaa gggccttgta
     2221 gatctggcgc cattcttcgg cggacttttc tgtgaaacgg cggtaaatca tgggcatgat
     2281 ttcgttgagt gtttggctaa gaacaaggag ggaaacgcaa gttagccgcg ttcgtataca
     2341 agcgagcagt tctcgagaac gtactagttg aaggtgccat cggcgatctc ttgcatcaaa
     2401 gtcgacgatg caccccaagg ttcattgttg gtggcctcgc gcacctgggt tcgctgtcag
     2461 ctcccgttcc atggtctctt gtctgggatc aagtatatgg cgaagggcgc aagagaatgg
     2521 tgccagtgtg gcgcgtggat acgggcatac cttggactcc atctcggtat agttcatgac
     2581 agctggttgg agccattgtc agcaccctga acacgaaatc atgagggagt cttggttctg
     2641 gactaggtgg tcacctacca ttttgaacct ttcgtacgcc agccttgatg tcgtagagtg
     2701 tcaggttgga cacggtatcc ttgaggctat tgaaatccat tgcgtaaacg tgtctatcgc
     2761 cggagttgct gggaattgtt ctgatgggct aaggtaattc ttctctagat ccaatctgag
     2821 cctcgcggtg atgattgcca gacgggcggg atgctgtggc gacttggtgg tttttgttcg
     2881 ggctgatcgc tttcagggcg acgtttcacg gtcttgtcgg gatcagatgg acattgaggt
     2941 gcggaattat acgatttagg tgactgcagg tagaaggagc agtccatctg cgtgaatcac
     3001 gagagaatca gctactttga atcgatggat gcagctacca gaagtcactc agttcgttca
     3061 aagccacatc actgggcact tccattggga caggcattga tcggacgaga ccgacttctg
     3121 gccgctttca acagccacat tatatccatg tcacggctac gcgtcggcct tcggtaacag
     3181 aaaagcacac agacagcgat tgtgacatgg attcgggcaa agcattggtg gtcgcaccag
     3241 gtcatcctga gtgtgcagtg gctgctattc agatttcatc taactgcggg agaggggttc
     3301 aaaggggcgt gacgtcacag acaacgggtg aaggacgaag attgcctcac ttctttgcta
     3361 gcaatttgcc tgcaaagaag gcgcacatga caagcaaaca actgggaaac cactattgaa
     3421 tacccacaat gcaaagctcg gaaggtacgt cttgattgca gtgtgtcgag tgtcaaaaaa
     3481 gaagcaagtg ttcatgcaag ccaaaattgg cacctcctcc acttctccga gtgccccacc
     3541 cgaacctcca ggcgagatgg ccggaacata ccatccgcgt tgggattatg acgtatctcc
     3601 ttcttcttca catgattcca tcccgttgtt gcttgtttgc gagctgtgac gggagatcgt
     3661 agatgccact tcgggccagg caggcagtgc aggcagccag gaacacaagc ttccaacttg
     3721 gtcatctcga ttgccgatta agggaaccaa atgcctatat aagactgtcc tcccacctcc
     3781 ccaataccat tcttttcttc ttccatcatc agccaacaaa gcaatcacat cttcactact
     3841 tcaaatcaac acaacactca aaccactttc acaacccctc acatcaacca aatctagaac
     3901 tagtggatcc accatgcctc ccaagaagac cgagaccaag gccgccgatg cctctgccgc
     3961 cgccgctcct gctcccgccg ctgctcccac ctctgctccc aagaccaaga gccccagcac
     4021 ccatgcctct taccttgtaa gctttactta atccccaaga gaaacgcatc ttttgctaac
     4081 attcaaacag gacatgatca cggacgctat tgttgcggta tttaaacccc ctcattatct
     4141 ttgttgtggc gtgttttggt tgtgggcgct tctctggtcg gtgagcaaaa tgagacaatt
     4201 gctctcatga tgcacgcaac caagagtcac ccacaccact caccaccctt ttactgtccc
     4261 tcagtcgtgc tgattccttc tttttgtcat gctacagctc aaggaccgcg ctggatctag
     4321 gtatgtgcca tttttctgct tacttttctc attttttcac cagggctttg ccaagccaca
     4381 ccgcaaacct tgcgggccct tcctacggtc gcaccgatga ccactttcat tcttatttcc
     4441 atgacgcgat gaaggatcac aacatgttgc taacgcacac cacagccgtc aagctctcaa
     4501 gaagtatgtc cgtgccaaca acacgctcgg caacgtcacc gacaacatgt tcgactcgct
     4561 cttcaacaag gccttgaaga acggtgtcga caagggtgtc tttgagcagc ccaagggtat
     4621 gtcaccaccc acatgacgca tcatctcctt gtattactct ttgcatgctc acacacgata
     4681 taggtccttc cggtggcacc aagcttgcca agaaggtcgc taagcctgct cccaagaagg
     4741 ctgctcccaa gaaggagacc aaggagaaga agcccgctgc cgctaagaag gagggtgccg
     4801 ccaagaagga gaccaaggag aagaaggctc ctgctgccaa gaaggcggct gcccccaaga
     4861 aggctgctgc tcccaagaag gaggtcaagg agaagaaggc ggctgccccc aagaagaagg
     4921 ccgctgctcc tgccgttgcc gacaaggaga ccgtcctcac caagaccaag tctggccgtg
     4981 tcgccaagag caccgctaag cctgctgccg ccaagaaggc cgctgctccc aagaaggctg
     5041 ccgccagcaa gaaggccgag aaggccgagc ccgctgccga gaaggcatta attaacatgt
     5101 ccgccatcaa gcccgacatg aagatcaagc tccgcatgga gggcaacgtc aacggccacc
     5161 acttcgtcat cgacggcgac ggcaccggca agcccttcga gggcaagcag tccatggacc
     5221 tcgaggtcaa ggagggcggc cccctcccct tcgccttcga catcctcacc accgccttcc
     5281 actacggcaa ccgcgtcttc gccaagtacc ccgacaacat ccaggactac ttcaagcagt
     5341 ccttccccaa gggctactcc tgggagcgct ccctcacctt cgaggacggc ggcatctgca
     5401 acgcccgcaa cgacatcacc atggagggcg acaccttcta caacaaggtc cgcttctacg
     5461 gcaccaactt ccccgccaac ggccccgtca tgcagaagaa gaccctcaag tgggagccct
     5521 ccaccgagaa gatgtacgtc cgcgacggcg tcctcaccgg cgacgtcgag atggccctcc
     5581 tcctcgaggg caacgcccac taccgctgcg acttccgcac cacctacaag gccaaggaga
     5641 agggcgtcaa gctccccggc gcccacttcg tcgaccactg catcgagatc ctctcccacg
     5701 acaaggacta caacaaggtc aagctctacg agcacgccgt cgcccactcc ggcctccccg
     5761 acaacgcccg ccgcggtaag cctatcccta accctctcct cggtctcgat tctacgtgac
     5821 tagaggatcc gacactatag aatacctaga cagtttgcag gtcatgtgaa tgttatcgcc
     5881 aacacattga atgctaaaca gattacctcc aacgcctaga tgaaaccaaa ttacccttgc
     5941 cttctttttt tttacgccgt taatgcagtt gtgatagaga ccatgcaacg tcttctaaac
     6001 ggagacacaa acacattcga gtcgatcaga tcgaaatgac ttgttcctgt acctcgtcac
     6061 catccagcca ccgtctgaaa tagacgcagt tgctggggct gacaggtccg ccttcctcgc
     6121 acatatcata gacagggcac cgcccacaag gcgcctccat aaggccactt gcactcggct
     6181 caactcccat aaatgaggta acaatgcctc ctttggttgg atcatggtcg aggcgtcgtt
     6241 gattgcacgt cctcggatcc tgtttgggga tgcgcgtcac acggtacccc ttgcgcccat
     6301 tgaccttgac aggctctact aagccatcgt agtatagcac atccaccaac tgctggatat
     6361 ccgcctcgct caacgtcgta ttattcgtga tgccggcgtg gtggatgaac tttgcaatgt
     6421 ccgacacagt cgggtacgac ttgtaaccgg ccgggagagg cagcaattgc ggcttgccgg
     6481 gcctggtctt cttggccggt gccggtgccg ccgtgtcgtc gttggaaatc tcgcccgcgc
     6541 tgcgcttcgt ccccttggaa atatcctccg agtggggaat ggtcccctga accacgccct
     6601 tcttgggatt cttttcacgc tgctgtgctc ctcctccttg tgctcctact cctggtactc
     6661 cagcaccgcc gccgtgggaa gtatatgtcg atttctgctt gataaagtca aacacaattt
     6721 cctgtagctc gttgatgaag gccgtgtcga gctcgccctc agtaaaccag gggccacctg
     6781 tggcgcgatc ggagggacgt aggttcgcct tgatgtacat cttcttgttg gggtgctcga
     6841 cgttcttcat gctggcgatg tagccctttt gctcgagata cttgatgcaa tgcttcataa
     6901 gcgagtcggc catgttgagc ttgttcttga tggtccggtt ccagatgccg tcggccccgg
     6961 cttcgtcgac catggcgtat accatcatta tttcggagtt ggggagggag gtgtaccttt
     7021 tgggaggaag gggaggttag ttggtgtgct cggttggctg tttcctgtgt gaaattgtta
     7081 tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc
     7141 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7861 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9181 atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat
     9241 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     9301 aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca
     9361 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     9421 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt
     9481 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
     9541 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
     9601 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
     9661 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
     9721 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
     9781 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
     9841 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
     9901 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
     9961 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
    10021 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
    10081 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
    10141 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
    10201 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
    10261 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
    10321 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
    10381 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
    10441 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
    10501 gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca
    10561 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
