LOCUS       Exported                7751 bp ds-DNA     circular SYN 07-DEC-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(1083..7751,1..1082)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        33..137
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             138..998
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      1169..1757
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1940..2032
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    2045..2066
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        2081..2111
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    2119..2135
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(2199..2250)
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=polyA_signal"
     promoter        2260..2281
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     polyA_signal    complement(2285..2509)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(2536..2601)
                     /label=dmpM (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.9"
                     /note="/other=CDS"
     CDS             complement(2651..3250)
                     /label=PuroR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(3283..3745)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     intron          3922..4151
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             complement(4309..6159)
                     /label=p300 core (D1399Y)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    5104..5106
                     /label=D1399Y
     CDS             complement(6193..6906)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
     CDS             complement(6937..7215)
                     /label=FRB
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7238..7449)
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(7475..7493)
                     /label=small t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
     enhancer        7499..7518
                     /label=CMV enhancer (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.3"
                     /note="/other=enhancer"
     misc_feature    7673..7725
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
ORIGIN
        1 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
       61 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
      721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
      781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1861 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1921 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1981 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2041 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     2101 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     2161 atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa gatacattga
     2221 tgagtttgga caaaccacaa ctagaatgca ctcgagcccc agctggttct ttccgcctca
     2281 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
     2341 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
     2401 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
     2461 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     2521 cagcgagctc tagagtcggt gggcctcggg ggcgggtgcg gggtcggcgg ggccgccccg
     2581 ggtggcttcg gtcggagcca tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt
     2641 ggggcgggcg tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca
     2701 cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg
     2761 cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca
     2821 cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccggga
     2881 accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg
     2941 ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg
     3001 cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca
     3061 ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct
     3121 cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg
     3181 cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt
     3241 actcggtcat ggaaggtcgt ctccttgtgg gttgtggcaa gcttatcatc gtgtttttca
     3301 aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac
     3361 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
     3421 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
     3481 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
     3541 tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt
     3601 cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt
     3661 caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa
     3721 gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa
     3781 catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg
     3841 gagggagagg gggggggaga ggggcggaat tggccgccct agatgcatgc tcgacctgca
     3901 gttggacctg ggagtggaca cctgtggaga gaaaggcaaa gtggatgtca ttgtcactca
     3961 agtgtatggc cagatctcaa gcctgccaca cctcaagctt gacaacaaaa agattgtctt
     4021 ttctgaccag atggacgcgg ccaccctcaa aggcatcacc gcgggccagg tgaatatcaa
     4081 atcctcctcg tttttggaaa ctgacaatct tagcgcagaa gtcatgcccg cttttgagag
     4141 ggagtactca ccccaacagc tggccctcgc agacagcgaa ttaattccag cacactggat
     4201 cagttatcta tgcggccgcg agctcggatc cactagtaac ggccgccagt gtgctggaat
     4261 tctgcagata attcgtcaag aagcgtagtc cggaacgtcg tacgggtagt cctggctctg
     4321 cgtgtgcagc tccaccagca tgcacatggt ggaccactgg gctcttcgga gtgaagagaa
     4381 ctccaggtgc ttgtcccttg ccagcgtgag aaacgcatcc cgaccatcca tcagatcgca
     4441 ggggatgaga ggatcaggat caacaatggg aggcagggag ttggcagcag ggccagcaat
     4501 gaggcggatc acaaagaaga cctctttatg cttctccatg gtggcatata gtttctgtga
     4561 gaggtcgtta gatacattgg gcatcccggg tttcttcttg ttgcccctac tcaggctgct
     4621 cttatttttg ctggttttct tattattctt ctttttagca tttttgctgt ctcccttggt
     4681 cacatctgtg ctttcattgc tggtgttttc ctctcgtttt ctctcttctt cctcctgttc
     4741 cagttcctta atgctttctt ccagaacatt gggccagaaa tcaccctcga aataaggcaa
     4801 ttcctttgca cttgttaatc tatcttcagt agcttgttta aaaatatcct tgtagtcatg
     4861 gacaatacgc tctgatacag ccttgtcaag catttttttg taccattcct gcagtcgctt
     4921 gggcttgggt atcttctggt caggaggatg gcaatggaag atataatcat ctccctcact
     4981 tggtggacat gcccaaatat gccctgttgt gtaacctaat ttcttgacat attctaaata
     5041 tccaattagg atttcatgat agactgcagt cctcaagcat ttaggacgga agaaatgaac
     5101 actatagagg taagatatgt atactctcct ctggttgggt ggagggcagt cagagccata
     5161 ctcttgaaca tgcatgccaa agaagcacag gtcaacacca tcaatttctt caaaggcaaa
     5221 gagggctttg gttcggtatg gaaaggattc tgccatctct ccactgtcca caaaccttgc
     5281 tttcatgcct ggttttactt ccacggtttt gtcagaagca tgaactactc taacagtgac
     5341 ctctcctgac tcagggtgat tctgtcgcct cagaaagtca ttcacacgat tctctagaaa
     5401 ggtgccaagt ctggtagatg gcaacctttt agcagaaaac ttattttctt tcctagttcg
     5461 tgcacttttc tttaaacagc catcacagac gaatccagca ggccagatga tctcatggtg
     5521 aaggacacag atctgatgca tctttcttcc gcactctgta cattcaacaa acagttcagg
     5581 atccagtgtg tcattttttc tcttggaaaa ttgttcttta tttattgtag tttgaggctg
     5641 ggaagggtca tcccccaaag aaacgctctc cccttggatc tcattgaaac acttctcaca
     5701 gaaatgatac ctgttctggt aactgtaata agtggcatca cgaggtattg tgcacaactg
     5761 tttgccgtag caacacagtg tctgtggaga gaactccaac tttctgccac aacagtatcc
     5821 aaggctttgc atcactgggt caatttcttg ttcaaagacc tcagagagct tggagcagta
     5881 tttgtatacc cgtgatgttt tccggttata taaccaggca ttattgaaca taagccaaat
     5941 atcatcgaca tactgccagg gctcctgata ctgtccagtg tctaacttcc tcttaatggt
     6001 agaaagatcc atggggctct tcacaatatc aaagtaatca gggattccta aaagctgagg
     6061 gtccacaggt tgacgaaagg gaagggattc tggatcctga cggtaaagtg cctccaaagt
     6121 tggcatcagt gcctgtcgta gttcttctgg tttgaaaatg gcgcgcccgc tagccttgga
     6181 tccggcaatt ggcttgtaca gctcgtccat gccgagagtg atcccggctg cggtcacgaa
     6241 ctccttaagg accatgtgat ctcgcttctc gttggggtct ttgctcaggg cggactgggt
     6301 gctcaggtag tggttgtcag gcagcagcac gggtccgtca ccgattgggg tgttctgctg
     6361 gtagtggtcg gcgagctgca cgcttccgtc ctcgatgttg tgacggatct tgaagttcac
     6421 cttgattccg ttcttctgct tgtcagccat gatatagacg ttgtggctgt tgtagttgta
     6481 ctccagcttg tgacccagga tgttaccgtc ctccttgaag tcgatgccct tcagctcgat
     6541 gcggttcacc agggtgtctc cctcgaactt cacctcagcg cgagtcttgt agttgccgtc
     6601 gtccttgaag aagatggtac gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     6661 gtcgtgctgc ttcatgtggt cggggtatcg gctgaagcac tgcacgccgt aggtcagggt
     6721 ggtcacgagg gtaggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     6781 cagcttgccg taggtagcat cgccctctcc ctcaccactc acactgaact tgtgaccgtt
     6841 tacgtcaccg tccagttcga ccaggattgg caccactccg gtgaacagct cctctccctt
     6901 gctcacggat cctccacctc cacctccgcc ggcctgcttt gagattcgtc ggaacacatg
     6961 ataatagagg tcccaggctt gggtgaggtc cttgacattc cctgatttca tgtacttcct
     7021 gcaccactct tgggcctcca ttaaatctcg accataggcc tgattaaagg atgtttcctt
     7081 cagagtctgg gggccccgtt ccatcatagc atgcaagggc tccagcacct caaacatgcc
     7141 tttcacgttc ctttccccaa agtacaaacg agatgcctct tccaggcctt catgccacat
     7201 ctcatgccag aggatcatgg tggctctaga accggtcctg tgttctggcg gcaaacccgt
     7261 tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg
     7321 gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct
     7381 aggcaccgga tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
     7441 gctctgccca ctgacgggca ccggagccaa ttcccactcc tttcaagacc tagctagcac
     7501 tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac
     7561 gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt
     7621 aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc
     7681 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc
     7741 tcccgatccg t
//
