LOCUS       Exported                7738 bp ds-DNA     circular SYN 07-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        30..134
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             135..995
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      1166..1754
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1937..2029
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    2042..2063
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        2078..2108
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    2116..2132
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(2196..2247)
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=polyA_signal"
     promoter        complement(2257..2278)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     polyA_signal    complement(2282..2506)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(2533..2598)
                     /label=dmpM (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.9"
                     /note="/other=CDS"
     CDS             complement(2648..3247)
                     /label=PuroR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(3280..3742)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     intron          complement(3919..4148)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             complement(4293..6143)
                     /label=p300 core
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             complement(6177..6890)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
     CDS             complement(6921..7199)
                     /label=FRB
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7222..7433)
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(7459..7477)
                     /label=small t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
     enhancer        complement(7483..7502)
                     /label=CMV enhancer (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.3"
                     /note="/other=enhancer"
     misc_feature    7657..7709
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
ORIGIN
        1 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
       61 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
      541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
      721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
      901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
      961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1921 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1981 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2041 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2101 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2161 attacgccaa gctctagcta gaggtcgacg gtatacagac atgataagat acattgatga
     2221 gtttggacaa accacaacta gaatgcactc gagccccagc tggttctttc cgcctcagaa
     2281 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     2341 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     2401 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     2461 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     2521 cgagctctag agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt
     2581 ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg
     2641 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     2701 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     2761 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     2821 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     2881 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     2941 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     3001 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     3061 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     3121 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     3181 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     3241 cggtcatgga aggtcgtctc cttgtgggtt gtggcaagct tatcatcgtg tttttcaaag
     3301 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
     3361 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
     3421 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     3481 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     3541 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
     3601 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
     3661 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
     3721 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
     3781 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt taggggggag
     3841 ggagaggggg ggggagaggg gcggaattgg ccgccctaga tgcatgctcg acctgcagtt
     3901 ggacctggga gtggacacct gtggagagaa aggcaaagtg gatgtcattg tcactcaagt
     3961 gtatggccag atctcaagcc tgccacacct caagcttgac aacaaaaaga ttgtcttttc
     4021 tgaccagatg gacgcggcca ccctcaaagg catcaccgcg ggccaggtga atatcaaatc
     4081 ctcctcgttt ttggaaactg acaatcttag cgcagaagtc atgcccgctt ttgagaggga
     4141 gtactcaccc caacagctgg ccctcgcaga cagcgaatta attccagcac actggatcag
     4201 ttatctatgc ggccgcgagc tcggatccac tagtaacggc cgccagtgtg ctggaattct
     4261 caagaagcgt agtccggaac gtcgtacggg tagtcctggc tctgcgtgtg cagctccacc
     4321 agcatgcaca tggtggacca ctgggctctt cggagtgaag agaactccag gtgcttgtcc
     4381 cttgccagcg tgagaaacgc atcccgacca tccatcagat cgcaggggat gagaggatca
     4441 ggatcaacaa tgggaggcag ggagttggca gcagggccag caatgaggcg gatcacaaag
     4501 aagacctctt tatgcttctc catggtggca tatagtttct gtgagaggtc gttagataca
     4561 ttgggcatcc cgggtttctt cttgttgccc ctactcaggc tgctcttatt tttgctggtt
     4621 ttcttattat tcttcttttt agcatttttg ctgtctccct tggtcacatc tgtgctttca
     4681 ttgctggtgt tttcctctcg ttttctctct tcttcctcct gttccagttc cttaatgctt
     4741 tcttccagaa cattgggcca gaaatcaccc tcgaaataag gcaattcctt tgcacttgtt
     4801 aatctatctt cagtagcttg tttaaaaata tccttgtagt catggacaat acgctctgat
     4861 acagccttgt caagcatttt tttgtaccat tcctgcagtc gcttgggctt gggtatcttc
     4921 tggtcaggag gatggcaatg gaagatataa tcatctccct cacttggtgg acatgcccaa
     4981 atatgccctg ttgtgtaacc taatttcttg acatattcta aatatccaat taggatttca
     5041 tgatagactg cagtcctcaa gcatttagga cggaagaaat gaacactatc gaggtaagat
     5101 atgtatactc tcctctggtt gggtggaggg cagtcagagc catactcttg aacatgcatg
     5161 ccaaagaagc acaggtcaac accatcaatt tcttcaaagg caaagagggc tttggttcgg
     5221 tatggaaagg attctgccat ctctccactg tccacaaacc ttgctttcat gcctggtttt
     5281 acttccacgg ttttgtcaga agcatgaact actctaacag tgacctctcc tgactcaggg
     5341 tgattctgtc gcctcagaaa gtcattcaca cgattctcta gaaaggtgcc aagtctggta
     5401 gatggcaacc ttttagcaga aaacttattt tctttcctag ttcgtgcact tttctttaaa
     5461 cagccatcac agacgaatcc agcaggccag atgatctcat ggtgaaggac acagatctga
     5521 tgcatctttc ttccgcactc tgtacattca acaaacagtt caggatccag tgtgtcattt
     5581 tttctcttgg aaaattgttc tttatttatt gtagtttgag gctgggaagg gtcatccccc
     5641 aaagaaacgc tctccccttg gatctcattg aaacacttct cacagaaatg atacctgttc
     5701 tggtaactgt aataagtggc atcacgaggt attgtgcaca actgtttgcc gtagcaacac
     5761 agtgtctgtg gagagaactc caactttctg ccacaacagt atccaaggct ttgcatcact
     5821 gggtcaattt cttgttcaaa gacctcagag agcttggagc agtatttgta tacccgtgat
     5881 gttttccggt tatataacca ggcattattg aacataagcc aaatatcatc gacatactgc
     5941 cagggctcct gatactgtcc agtgtctaac ttcctcttaa tggtagaaag atccatgggg
     6001 ctcttcacaa tatcaaagta atcagggatt cctaaaagct gagggtccac aggttgacga
     6061 aagggaaggg attctggatc ctgacggtaa agtgcctcca aagttggcat cagtgcctgt
     6121 cgtagttctt ctggtttgaa aatggcgcgc ccgctagcct tggatccggc aattggcttg
     6181 tacagctcgt ccatgccgag agtgatcccg gctgcggtca cgaactcctt aaggaccatg
     6241 tgatctcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
     6301 tcaggcagca gcacgggtcc gtcaccgatt ggggtgttct gctggtagtg gtcggcgagc
     6361 tgcacgcttc cgtcctcgat gttgtgacgg atcttgaagt tcaccttgat tccgttcttc
     6421 tgcttgtcag ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgaccc
     6481 aggatgttac cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     6541 tctccctcga acttcacctc agcgcgagtc ttgtagttgc cgtcgtcctt gaagaagatg
     6601 gtacgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     6661 tggtcggggt atcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtaggc
     6721 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggta
     6781 gcatcgccct ctccctcacc actcacactg aacttgtgac cgtttacgtc accgtccagt
     6841 tcgaccagga ttggcaccac tccggtgaac agctcctctc ccttgctcac ggatcctcca
     6901 cctccacctc cgccggcctg ctttgagatt cgtcggaaca catgataata gaggtcccag
     6961 gcttgggtga ggtccttgac attccctgat ttcatgtact tcctgcacca ctcttgggcc
     7021 tccattaaat ctcgaccata ggcctgatta aaggatgttt ccttcagagt ctgggggccc
     7081 cgttccatca tagcatgcaa gggctccagc acctcaaaca tgcctttcac gttcctttcc
     7141 ccaaagtaca aacgagatgc ctcttccagg ccttcatgcc acatctcatg ccagaggatc
     7201 atggtggctc tagaaccggt cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt
     7261 cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca
     7321 gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac cggatcaatt
     7381 gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg
     7441 ggcaccggag ccaattccca ctcctttcaa gacctagcta gcactagtca ataatcaatg
     7501 tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
     7561 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
     7621 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
     7681 agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcga
//
