LOCUS       pICH47781               5977 bp ds-DNA     circular     10-NOV-2022
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pICSL1P6_crU626.gb
            ApEinfo:methylated:1 Source name: Cas12gRNAexHPuchta+longer3'
            (Note: Geneious sometimes stores the sequence name in the source
            attribute) Source name: Cas12gRNAexHPuchta+longer3' (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     source          30..382
                     /label="U6-26 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /dnas_title="Cas12gRNAexHPuchta+longer3'"
                     /label="U6-26t(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    319..361
                     /label="hammerhead"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="hammerhead"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    362..382
                     /label="LbcrRNA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="LbcrRNA"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    390..1458
                     /label="RFP"
                     /ApEinfo_revcolor="#fc0012"
                     /ApEinfo_fwdcolor="#fc0012"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1466..1533
                     /label="HDV ribozyme"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="HDV ribozyme"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Term.           1534..1634
                     /label="U6-26t"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(1666..1793)
                     /label="RB\short"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /vntifkey=21
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(1817..3298)
                     /label="RK2\trfa\(no\Esp3I)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /vntifkey=21
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3309..4098
                     /label="pUC\ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /vntifkey=21
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     rep_origin      3344..3344
                     /label="ORI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="RNaseH cleavage point"
                     /vntifkey=33
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             complement(4106..4963)
                     /label="AP\r"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_fwdcolor="#e9d024"
                     /vntifkey=4
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      complement(5161..5778)
                     /label="oriV"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="shows similarity to GenBank Accession NumberM20134"
                     /standard_name="oriV"
                     /vntifkey=33
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5808..5955
                     /label="TDNA-LB"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="shows similarity to T-DNA left border: GenBankAccession Number J01825"
                     /vntifkey=21
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 tctgtgaaga caatgtggaa ttcctcgagG GAGTTTTTGT ATCTTGTTTC ATAGTTTGTC
       61 CCAGGATTAG AATGATTAGG CATCGAACCT TCAAGAATTT GATTGAATAA AACATCTTCA
      121 TTCTTAAGAT ATGAAGATAA TCTTCAAAAG GCCCCTGGGA ATCTGAAAGA AGAGAAGCAG
      181 GCCCATTTAT ATGGGAAAGA ACAATAGTAT TTCTTATATA GGCCCATTTA AGTTGAAAAC
      241 AATCTTCAAA AGTCCCACAT CGCTTAGATA AGAAAACGAA GCTGAGTTTA TATACAGCTA
      301 GAGTCGAAGT AGTGATTGaa attactgatg agtccgtgag gacgaaacga gtaagctcgt
      361 cTAATTTCTA CTAAGTGTAG ATTGAGACCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
      421 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
      481 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
      541 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAT ACTAGAGAAA
      601 GAGGAGAAAT ACTAGATGGC TTCCTCCGAG GACGTTATCA AAGAGTTCAT GCGTTTCAAA
      661 GTTCGTATGG AAGGTTCCGT TAACGGTCAC GAGTTCGAAA TCGAAGGTGA AGGTGAAGGT
      721 CGTCCGTACG AAGGTACCCA GACCGCTAAA CTGAAAGTTA CCAAAGGTGG TCCGCTGCCG
      781 TTCGCTTGGG ACATCCTGTC CCCGCAGTTC CAGTACGGTT CCAAAGCTTA CGTTAAACAC
      841 CCGGCTGACA TCCCGGACTA CCTGAAACTG TCCTTCCCGG AAGGTTTCAA ATGGGAACGT
      901 GTTATGAACT TCGAGGACGG TGGTGTTGTT ACCGTTACCC AGGACTCCTC CCTGCAAGAC
      961 GGTGAGTTCA TCTACAAAGT TAAACTGCGT GGTACCAACT TCCCGTCCGA CGGTCCGGTT
     1021 ATGCAGAAAA AAACCATGGG TTGGGAAGCT TCCACCGAAC GTATGTACCC GGAGGACGGT
     1081 GCTCTGAAAG GTGAAATCAA AATGCGTCTG AAACTGAAAG ACGGTGGTCA CTACGACGCT
     1141 GAAGTTAAAA CCACCTACAT GGCTAAAAAA CCGGTTCAGC TGCCGGGTGC TTACAAAACC
     1201 GACATCAAAC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTTGA ACAGTACGAA
     1261 CGTGCTGAAG GTCGTCACTC CACCGGTGCT TAATAACGCT GATAGTGCTA GTGTAGATCG
     1321 CTACTAGAGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT
     1381 TTATCTGTTG TTTGTCGGTG AACGCTCTCT ACTAGAGTCA CACTGGCTCA CCTTCGGGTG
     1441 GGCCTTTCTG CGTTTATAGG TCTCAGGCCG GCATGGTCCC AGCCTCCTCG CTGGCGCCGG
     1501 CTGGGCAACA TGCTTCGGCA TGGCGAATGG GACTTTTTTT GCAAAATTTT CCAGATCGAT
     1561 TTCTTCTTCC TCTGTTCTTC GGCGTTCAAT TTCTGGGGTT TTCTCTTCGT TTTCTGTAAC
     1621 TGAAACCTAA AATTCGCTga gcttgtcttc tgcacgaagt ggtttaaact atcagtgttt
     1681 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     1741 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     1801 ggttccccag atcaggcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc
     1861 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
     1921 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
     1981 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
     2041 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
     2101 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
     2161 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
     2221 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
     2281 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
     2341 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
     2401 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
     2461 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
     2521 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
     2581 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
     2641 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
     2701 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
     2761 gcctcctgtt caagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
     2821 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
     2881 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
     2941 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
     3001 cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat
     3061 tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag
     3121 tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc
     3181 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
     3241 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
     3301 taggatctgc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg
     4261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5041 agaaaaataa acaaataggg gttccgcgca cgaattggcc agcgctgcca tttttggggt
     5101 gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg
     5161 ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg
     5221 cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag
     5281 gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg
     5341 acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg
     5401 tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg
     5461 cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt
     5521 cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat
     5581 ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt
     5641 cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac
     5701 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg
     5761 cgagggcaac cagcccggtg agcgtcgcaa aggagatcct gatctgactg atgggctgcc
     5821 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     5881 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     5941 gtttttaatg tactggggtg gatgcagtgg gccccac
//