LOCUS       pBK-CMV-mpx        7625 bp    DNA     circular     13-DEC-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|609426701|
COMMENT     VNTDBDATE|609428666|
COMMENT     LSOWNER|
COMMENT     VNTNAME|mpx pBK-CMV|
COMMENT     VNTAUTHORNAME|Chansey Veinotte|
FEATURES             Location/Qualifiers
     misc_feature    24..330
                     /vntifkey="21"
                     /label=f1\origin
     primer          952..968
                     /vntifkey="27"
                     /label=M13_forward20_primer
     promoter        975..993
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4246..4265)
                     /vntifkey="30"
                     /label=T3\promoter
     primer          complement(4299..4321)
                     /vntifkey="27"
                     /label=M13\reverse\primer
     promoter        complement(4414..4485)
                     /vntifkey="29"
                     /label=CMV\promoter
     rep_origin      complement(5102..5721)
                     /vntifkey="33"
                     /label=pBR322\origin
     exon            complement(6316..7095)
                     /vntifkey="61"
                     /label=KanR
     promoter        complement(7190..7458)
                     /vntifkey="29"
                     /label=SV40\promoter
     exon            complement(1074..4181)
                     /vntifkey="61"
                     /label=mpx
BASE COUNT     2004 a      1839 c      1877 g      1905 t 
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg 
      481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 
      541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 
      601 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt 
      661 aaaacctcta caaatgtggt atggctgatt atgatcatga acagactgtg aggactgagg 
      721 ggcctgaaat gagccttggg actgtgaatc taaaatacac aaacaattag aatcagtagt 
      781 ttaacacatt atacacttaa aaattggatc tccattcgcc attcaggctg cgcaactgtt 
      841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 
      901 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 
      961 cggccagtga attgtaatac gactcactat agggcgaatt gggtacactt acctggtacc 
     1021 ccacccgggt ggaaaatcga tgggcccgcg gccgctctag aagtactctc gagttttttt 
     1081 ttttttttat tgacaaaaaa ctttatttat tagttttttt actttgctaa aagcatacac 
     1141 aaaaataact gtcacagcat ggactaccga aaattttgtt gcaatctgac aacaaaacca 
     1201 aaaacaacaa ataaaaaaaa aacttcataa aagaaatgtc gatgcattag cagttaaatc 
     1261 ttggttctga aattttatca cttatttacc tgtaaaaaat tctgaattaa aacataaggc 
     1321 attctctaac catactacta attttgcaaa gctaattatt aataaaacaa caacaacaac 
     1381 aacaacagag cctaagacat tacagtaaga agaaacatgt ttaaagacaa atagaaagaa 
     1441 gcagaatgca tttaaaatga acaaaaaaaa aagggaaata gtaatgcatt ataagtaata 
     1501 gcctcaaacg ggaaaaagaa tgtgaccaga gaaatagctt gaaaagccta taccattagc 
     1561 acttcgagaa acctccacat acacacggtc tcctctctgt aggctcagga ctgccttccc 
     1621 ttcagctaat gaccttgtat taagagcaac tgtttcagga gtaagctcta ccctattatt 
     1681 tttctttaag gtcacaaagc ccaaactacg agtccctatg catgaaaact cgaattcata 
     1741 tacaccaggt atctggcaca agaacatccc tgaggtttgg ttataatggt tttgtccatt 
     1801 gtaaaggacc ggaccgaaca ccacaacttt agcagtggca ggaaggatgg agttgactga 
     1861 ggcaaagaag gcagactgtt gtcctgttgt attaatggga ggtcctggaa ttcctggagg 
     1921 tcctggtggc cctgctacac cctgtggacc acgttctcct ggaggtcccc tgggtccggg 
     1981 aggtcctggg atggaatctc tatctcattt ccaatgtctg aaatgttgat gccaccgtca 
     2041 ccagtctctt tccaaggctc gagatcaaaa gctgggatat tcccacagtt aacaaaactg 
     2101 gcaggactcc ggaagcgaaa aggatctctg ggaactttaa ggattccggt gttgtcgcag 
     2161 attatgcggg ccattgacac agaagccaga gctgttttct gtttggtggt gaacactcca 
     2221 ttgctctcaa accacaacct atcgccatct cggattttct ggaactgtct tgaaatcagg 
     2281 caggcaaaaa gagaaccgac acgaccacca ggagcaaaag gctcagcaac acctcctaac 
     2341 caaacatcaa tgttctcagg ggtgccataa agctcaatta acttgcgggc tagtttagtg 
     2401 ttgttcatca ccacagccaa ttcttgctca ttctgaggag cagacaatcc acagaaccga 
     2461 cgccatgcat tataacctgg tatagcatgg tcacgccctc tttgcatgtt gagggaagcc 
     2521 agatccaaag ctatgtggga tgtgaaggca aacagcctct ctctgagagc attcaccaac 
     2581 atgtggtcct gggtgttcag ttttgccgga cgaccaatca atccacggag cacaggatca 
     2641 attccccctt caaagatcac cctccatgga gagaagaaag cctcatagag ggggacactg 
     2701 ggaaactgag gatggttctt gtagttttca tcaagacgaa aaatgaaggg ctggatagtc 
     2761 aagtgggcaa aacggaaagc agcagtggca aagacgttcg caatggtagg gtccacattc 
     2821 tcattgtaac ctggatatgg tccaaggtgt ctattgtaag catcagggcc tacaattagt 
     2881 ggcaagtatt ccttgatcac caagatctgg ttgaatgcac caacaatttt tcttgcttct 
     2941 tgataaagag tttcactact ccatgtaggg ttgagcacat gaagggcacg agccaatcta 
     3001 ttgtgctccc ttacaaaaag tgtgtgtaag gagttcagag caggattctc atcgacacga 
     3061 gcatcacccg caatgaagca aggaacctct gtcagagtgc tgtcgttgag gattttctga 
     3121 cgagtggcgc acaagttggt gttaacacta gtaaagggca ggagctctcg tccattgtct 
     3181 ttaaaacgat tattgacacg taaaagacca ccatcattag taaggtcccg cagttccttt 
     3241 gctagtccat cctctgaacc gtaaacttgt ccagcatcta ggtaagctgt tagagtattg 
     3301 atctgttccc gaacatttgg gacttcacca aacatgtagg cagtgtgccc agaaccacaa 
     3361 gtcggtgctg accggaagac agggagacag gtgttagggc gcagacgctg atctcctgga 
     3421 ggggccgaaa ttgggaagca gggttcagag cgctcacaac tgttttcaca gtcaatgcca 
     3481 ttactaaagg acctaatact tggtgagaaa ggtgtaaaag tcagatcgtg gtcaacccat 
     3541 tgcccaaaga tggtgagcat gaaggtaaag tcatggtcac tctctatgtc agcatctgca 
     3601 gtggctagaa tgcgattgga aaccagtcta accatgggca gcgctgcacc attgtacaac 
     3661 ttgttgggat cccatccttt tggctgagaa acggcgtcct catattgagc aggcaaccag 
     3721 cgggcaaatg gagtgttgga tgcaccaaga agtgggttct tccgattgtt gcagacaccg 
     3781 ctggcagttc gatacttgtt tatgaggggt gtggtcctac aagatggtgg ccgggtttga 
     3841 gcttcacagc ctgtcatacg tgtaatggtt tgaagctcgt cttgagtgag caggtttgtg 
     3901 gcattgatgg acctcttgtg tacatgatgg gctttctcac tgatcagtct cagcgtttgt 
     3961 gccatgtagt ctgcagctct tacggcctcc cgtgtctttc gagctggctg tttcagcaga 
     4021 cgtagcttgt cagaaggctt aatgacatct ttgcgcaccc ttgctaaact ctcatctcga 
     4081 gaatacttgt aagcagcgtc cactattttc tttgcttcct caattgaatc aagaataaaa 
     4141 ggtcttccag gactttcttc ttctgccccc actgaaagag cctcgtgccg aattctttgg 
     4201 atccactagt gtcgacctgc aggcgcgcga gctccagctt ttgttccctt tagtgagggt 
     4261 taatttcgag cttggcgtaa tcaaggtcat agctgtttcc tgtgtgaaat tgttatccgc 
     4321 tcacaattcc acacaatata cgagccggaa gtataaagtg taaagcctgg ggtgcctaat 
     4381 gagtgagcta actcacagta attgcggcta gcggatctga cggttcacta aaccagctct 
     4441 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt 
     4501 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc 
     4561 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta 
     4621 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt 
     4681 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt 
     4741 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt 
     4801 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg 
     4861 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc 
     4921 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt 
     4981 aattgattac tattaataac taatgcatgg cggtaatacg gttatccaca gaatcagggg 
     5041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     5101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     5161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     5221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     5281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     5341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     5401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     5461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     5521 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     5581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     5641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     5701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     5761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     5821 aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc 
     5881 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg 
     5941 gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag 
     6001 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt 
     6061 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg 
     6121 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga 
     6181 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg 
     6241 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc 
     6301 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc 
     6361 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc 
     6421 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc 
     6481 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc 
     6541 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag 
     6601 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc 
     6661 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt 
     6721 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc 
     6781 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc 
     6841 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag 
     6901 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt 
     6961 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc 
     7021 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 
     7081 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat 
     7141 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg 
     7201 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat 
     7261 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg 
     7321 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc 
     7381 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 
     7441 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg 
     7501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     7561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     7621 agtgc 
//
