LOCUS       pKS235                  5304 bp    DNA     circular     14-Sep-2022
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     Origin          complement(138..444)
                     /label="Origin f1_replication_origin"
                     /note="Ori_f1"
     Reporter        complement(462..622)
                     /label="Reporter lacZ_alpha_fragment"
                     /note="lacZ_a"
     Promoter        626..644
                     /label="Promoter T7_promoter"
                     /note="Pr_T7"
     Promoter        complement(3118..3137)
                     /label="Promoter T3_promoter"
                     /note="Pr_T3"
     Promoter        complement(3207..3236)
                     /label="Promoter lac_promoter"
                     /note="Pr_lac"
     Origin          complement(3545..4164)
                     /label="Origin pBR322_replication_origin"
                     /note="Ori_pBR322"
     Selection       complement(4319..5179)
                     /label="Selection ampicillin_resistance_gene_ORF"
                     /note="amp"
     Promoter        complement(5221..5249)
                     /label="Promoter ampicillin_resistance_gene(b-lactamase)promoter"
                     /note="Pr_amp"
     misc_feature     1081..2722
                     /label="peel-1 CDS+introns"
     misc_feature     2729..3052
                     /label="tbb-2 3'UTR"
     misc_feature     677..1074
                     /label="hsp-16.41 promoter + 5'UTR"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca 
      661 ccgcggtggc ggccgcgatc accaaaaacg gaacgttgag ctggacggaa atagtggtaa 
      721 agtgacatga ttatagtttg aagatttcta atttcacaat tagagcaaat gttgttcggt 
      781 atttattttc aacggtattt atactatttt ccaccttttt ctagaacatt cgagctgctt 
      841 gttgcaaaag gagggcgact cacattcggt acatggaaaa gtagtgtaca caataaagag 
      901 acccagatac attttccgtc tgcgtctctt tgcacccacc gggagtattt tcaaacgaat 
      961 gcatctagga ccttctagaa cattctgtaa ggctgcagaa tgcgggtata taaggaaagc 
     1021 gggctcagag gaagccaaca cgctttgttc tagtgcatct aaaaaacttc gaaaactagt 
     1081 acaaggatgc gctttgattt ccaaaactta aaattttcga tgattttcat atttttgtgg 
     1141 aacatcatag tcggattttt acttgctctt gtaaagattt tcagttgagt tgatttttta 
     1201 atatacacca attgcaatat tattcacttt cagaaatcta catgtatctt gatctgcctg 
     1261 aaaatgtatg ggtaaatcgg tttgcgcatg ttattgctct tattggatca attgcttcat 
     1321 tgtggttaat tttttttagc ccatttggtg agtttcataa atgttacaaa aatgggtgga 
     1381 aatggcttaa tcatgctgca ttaaacaaca aaattcaaca aaaaatcagt ttgaaacgta 
     1441 gatgaaaaga gctccaacat acaaatgggc gccaactctt gctatgcatt agataagaga 
     1501 gactctgata ctaatttttc actgggcgtg aaaaacgcga aggataactg tttgtcgtct 
     1561 gcgtctcttc gttcacacgc tattttcttc gagagaacta cgcgaaccgt ggacgaagaa 
     1621 tcctcctctt ccaccgcttt caaggccgtt ttgctcactt ttctaacgtt ttctcgtccg 
     1681 cgagaaaaca gcaattttga gacagccagc gggcccgtgt cccgtgtttt tccgtctttt 
     1741 gcgggtagcc acagctgatt tgccgtttgt aaaatactta tggtcaccaa tatttcagat 
     1801 gtattttgaa aatcgcgcat ttcgctgcgg gatcactaac attttaggag tattttgttt 
     1861 actctcgttg gtcgcacttt gctgaatatt tgcgattttg aaattatttt actatatcgc 
     1921 tggtgccttt tgtagaagtg cgcgaactac attttataag tattttaaaa accgcaaaac 
     1981 atctgtgagt tatgctatat gaccggcaac gacctccgac gaggttcgcg gctctctcac 
     2041 aaccactcag ttatttttga aagtaagagt ccaatagttc gtagatctgc atttgctaat 
     2101 taaaaaaagg aaaacctcaa aacactcaat gtttttttag aaattaagca aggaagattt 
     2161 tctgtctcgt caaaaactat cctgcttctt gtttgcaagg gtttgattgg cttgatattt 
     2221 cttgcacaga ttttcgccca tgtcctttac attgacccat gccagaatag taagtaaaca 
     2281 aaaccgcaca acaagaatgt gtatctcata ttgtcgagaa aaaatgatcg caatattttt 
     2341 gcaatgcttt attttgatct acctggtcaa aaaagactcc gcttcccaaa ctttctaaaa 
     2401 atgtttttct ctaagatact gttttgttcg ataaagagaa aggaggagtc cagtgcaccg 
     2461 ggtagatcaa aaataaattt ttcgaaagct ttgcgatcgt atttttccga taatagctaa 
     2521 actactttta tttacagtgc tcttatcatt gaactctgca ctgttctcct tgctgctgaa 
     2581 ttattatgag tctcgtggtg cgatccatcc gttgatggaa ttagttcaaa cagctggcct 
     2641 gagaccaact ttgtgcgctg ctgtacgatt taattgtctc aactcaactg ctcatattga 
     2701 tccaagtgtt gaaaatccat gacccgggat gcaagatcct ttcaagcatt cccttcttct 
     2761 ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg cttttttaat 
     2821 gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg aagtgaatgc 
     2881 tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc cattttgaag 
     2941 tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta ttgtgttcgt 
     3001 tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag tcgaattcga 
     3061 tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc 
     3121 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     3181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     3241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     3301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     3361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     3421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     3481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     3541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     3601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     3661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     3721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     3781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     3841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     3901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     3961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     4021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     4081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     4141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     4201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     4261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     4321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     4381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     4441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     4501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     4561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     4621 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     4681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     4741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     4801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     4861 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     4921 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     4981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     5041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     5101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     5161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     5221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     5281 cgcgcacatt tccccgaaaa gtgc
//