LOCUS       5_to_2144_of_PAM        2354 bp    DNA     circular SYN 08-Jun-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      30..73
                     /label="rrnB_T1_terminator"
                     /note="/translation=""MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVF
                     RLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"""
     rep_origin      complement(263..882)
                     /note="pBR322 origin"
     CDS             complement(1079..1873)
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(1082..1870)
                     /gene="NeoR/KanR"
                     /label="NeoR/KanR"
                     /note="/translation=""MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVF
                     RLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"""
     promoter        complement(1962..1999)
                     /label="NEOKAN_promoter"
     unsure          2071..2094
                     /label="<Description>TruSeq F"
     unsure          2115..2135
                     /note="Restriction sites for Golden Gate cloning"
     unsure          2148..2153
                     /label="<Description>Barcode 1"
     primer_bind     2154..2177
                     /label="<Description>TruSeq R"
     unsure          2154..2177
BASE COUNT  559 A  666 C  619 G  510 T  0 OTHER
ORIGIN
        1 AATTGTGGTA CGCGTGCTAG AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG
       61 CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC
      121 CCTAGACCTA GGGCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
      181 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
      241 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
      301 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
      361 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
      421 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC
      481 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
      541 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
      601 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
      661 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
      721 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
      781 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
      841 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
      901 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GACTAGTGCT TGGATTCTCA
      961 CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA AATCCAGATG GAGTTCTGAG
     1021 GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCT CGAACCCCAG AGTCCCGCTC
     1081 AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC
     1141 CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG
     1201 TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC
     1261 CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA
     1321 CGAGATCCTC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA
     1381 GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC
     1441 GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG
     1501 TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG
     1561 ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG
     1621 TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG
     1681 CTGCCTCGTC CTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG
     1741 GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG
     1801 CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT
     1861 CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCAGATCTT GATCCCCTGC
     1921 GCCATCAGAT CCTTGGCGGC AAGAAAGCCA TCCAGTTTAC TTTGCAGGGC TTCCCAACCT
     1981 TACCAGAGGG CGCCCCAGCT GGCAATTCCG ACGTCTAAGA AACCATTATT ATCATGACAT
     2041 TAACCTATAA AAATAGGCGT ATCACGAGGC GTACGACTCC ATGTTATGTA TGCCCCTTTC
     2101 GTCTTCACCT CGAGAGGTTG AGACCAAAGG TCTCGGTGGG GATCCGTCTT TCAGTTGCCT
     2161 CGCTGTACTA ATAATGGCCG ACAGATCGTC AAGATTAGAA AACGGTAGCA GCATTATCGG
     2221 AGGTTCTCTA TCTAGTATGG ATAGCCGTGT CTTCACTGTG CTGCGGCTAC CCATCGCCTG
     2281 AAAACCAGTT GGTGTTAAGC GATCCCCTGT CCAGGACGCC ACACGTAGTG AAACATACAC
     2341 GTTCGTCGGG TTCC
//
