LOCUS       Exported                5133 bp ds-DNA     circular SYN 29-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 15, 2022 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=5'-ITR
                     /note="/vntifkey=34"
     misc_feature    238..613
                     /label=TRE3G Promoter
                     /label=TRE3G\Promoter
     regulatory      620..629
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             626..1336
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      881..890
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    1343..1884
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    1891..2105
                     /label=BGH pA
                     /label=BGH\pA
                     /note="/vntifkey=25"
     repeat_region   2193..2322
                     /label=3'-ITR
                     /note="/vntifkey=34"
     primer_bind     complement(2341..2358)
                     /label=M13-fwd
     CDS             2429..2497
                     /label=LacZ alpha
     CDS             3086..3946
                     /label=Amp-R
                     /note="/vntifkey=4"
     rep_origin      4041..4723
                     /label=ColE1 origin
     misc_binding    5063..5085
                     /label=LacO
     primer_bind     5091..5111
                     /label=M13-rev
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc cacgaggccc tttcgtcttc actcgagttt
      241 actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg
      301 tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag
      361 tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt
      421 actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg
      481 tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt
      541 cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc
      601 ctaccctcgt aaagtcgacg ccaccatggt gagcaagggc gaggaggata acatggcctc
      661 tctcccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat
      721 ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac
      781 caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca
      841 tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc
      901 cggctaccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta
      961 ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt
     1021 ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggtcgaa
     1081 gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt acaccactgg
     1141 aaatggcaag cgctaccgga gcactgcgcg gaccacctac acctttgcca agccaatggc
     1201 ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc tcaagcactc
     1261 caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga tgggcatgga
     1321 cgagctgtac aagtaaacta gtaatcaacc tctggattac aaaatttgtg aaagattgac
     1381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     1681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     1741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     1801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     1861 tctgcggcct cttccgcgtc ttcgggatct gcctcgactg tgccttctag ttgccagcca
     1921 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1981 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2041 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2101 ggggactcga gttaagggcg aattcccgat taggatcttc ctagagcatg gctacgtaga
     2161 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     2221 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2281 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agccttaatt aacctaattc
     2341 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2401 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2461 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     2521 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     2581 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2641 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2701 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccccgat agacggtttt
     2761 tcgccctttg acgctggagt tcacgttcct caatagtgga ctcttgttcc aaactggaac
     2821 aacactcaac cctatctcgg tctattcttt tgatttataa gggatttttc cgatttcggc
     2881 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2941 aacgtttata atttcaggtg gcatctttcg gggaaatgtg cgcggaaccc ctatttgttt
     3001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaatagtgg
     3241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3601 aaacgacgag cgtgacacca cgatgcctgt agtaatggta acaacgttgc gcaaactatt
     3661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4261 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4741 cttttgctgc ggttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4801 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4861 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4921 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4981 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5041 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5101 ctatgaccat gattacgcca gatttaatta agg
//
