LOCUS       pLKO-Tet-ON-shCt        9142 bp DNA     circular SYN 12-SEP-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Friedel lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2022 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     426..444
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
                     /note="color: black; sequence: GTGTTCCGCATTCTGCAAG"
     intron          516..1088
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
                     /note="color: #------"
     primer_bind     1096..1115
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
                     /note="color: black; sequence: GCAACGTGCTGGTTATTGTG"
     promoter        1143..1161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1172..1789
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     primer_bind     complement(1245..1264)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
                     /note="color: black; sequence: GGCGAGTTTACGGGTTGTTA"
     misc_feature    1827..2400
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     primer_bind     complement(1994..2011)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
                     /note="color: black; sequence: GCATTCCTTTGGCGAGAG"
     primer_bind     2221..2240
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
                     /note="color: black; sequence: TGGCTCTCCTCAAGCGTATT"
     CDS             2407..3201
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2461..2480)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
                     /note="color: black; sequence: GCCCAGTCATAGCCGAATAG"
     primer_bind     3071..3090
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
                     /note="color: black; sequence: CGTTGGCTACCCGTGATATT"
     protein_bind    complement(3230..3329)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     LTR             3473..3706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    3778..3899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(3815..3834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     3869..3888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     rep_origin      3939..4074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4001..4020
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(4024..4035)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     promoter        complement(4095..4113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(4123..4140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     complement(4123..4139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4132..4154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     rep_origin      4281..4736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(4368..4387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     4578..4599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     promoter        4762..4866
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4867..5727
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4867 .. 4935 / #ccffcc / signal sequence
                        2: 4936 .. 5727 / #ccffcc
                     Cleavage site after base 4935"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5085..5104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      5898..6486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     6387..6406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     6640..6657
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     protein_bind    6774..6795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        6810..6840
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 6810 .. 6815 / #ffffff / -35
                        2: 6816 .. 6833 / #ffffff
                        3: 6834 .. 6840 / #ffffff / -10"
     protein_bind    6848..6864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     6853..6875
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     6872..6888
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     6872..6888
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     6907..6927
                     /label=T3
                     /note="T3 promoter, forward primer"
                     /note="color: black; sequence: GCAATTAACCCTCACTAAAGG"
     promoter        6909..6927
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6955..7181
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             7182..7362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    7409..7534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    8027..8260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     primer_bind     8608..8637
                     /label=pLKO-shRNAseq
                     /note="color: black; sequence: 
                     ggcagggatattcaccattatcgtttcaga; added: 2022-08-04"
     primer_bind     8789..8808
                     /label=H1
                     /note="Human H1 promoter, forward primer"
                     /note="color: black; sequence: TCGCTATGTGTTCTGGGAAA"
     protein_bind    8826..8844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    8854..8872
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    8875..8932
                     /label=shRNA Ctrl
                     /note="color: #ff0000"
     misc_feature    8933..8936
                     /label=Stuffer
                     /note="color: #a6acb3"
     misc_feature    8973..9090
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        join(9139..9142,1..507)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt
      541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
      841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
      901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
      961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
     1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
     1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     1801 cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa cgttactggc
     1861 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     1921 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     1981 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2041 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     2101 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     2161 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     2221 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     2281 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     2341 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     2401 ggatccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     2461 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     2521 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     2581 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     2641 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     2701 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     2761 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     2821 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     2881 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     2941 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3001 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3061 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3121 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3181 cttcttgacg agttcttctg aggtaccttt tttaagggtg ggcgcgccga cccagctttc
     3241 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     3301 actatcagtc aaaataaaat cattatttgc catccagctg atatccgccc gccccacgac
     3361 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg
     3421 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3481 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3721 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3901 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3961 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     4081 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4141 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4201 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4261 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4321 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4381 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4441 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4501 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4561 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4621 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4681 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4741 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4801 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4861 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4981 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5041 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5101 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5161 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5281 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5401 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5461 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5521 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5701 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5761 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5821 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6781 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6841 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6901 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6961 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     7021 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     7081 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     7141 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     7201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7381 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7441 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7501 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7561 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7621 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7681 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7741 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7801 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7861 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     8041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     8101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     8641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     8701 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     8761 agcctcgagc ggccgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg
     8821 tgaaatccct atcagtgata gagacttata agttccctat cagtgataga gacaccggat
     8881 ctcgcttggg cgagagtaag ctcgagctta ctctcgccca agcgagattt ttaattctcg
     8941 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     9001 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     9061 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     9121 ctttggccgc ggctcgaggg gg
//
