LOCUS       Exported                2442 bp ds-DNA     linear   SYN 20-OCT-2022
DEFINITION  LR071913WTShnV5C1_T7 LR071913WTShnV5C1.0.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 20, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             37..57
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 54"
                     /translation="ENLYFQG"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    complement(1988..2012)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     polyA_signal    2037..2261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 cctatcccta accctctcct cggtctcgat tctacggaaa acctgtattt tcagggcccg
       61 gatccgtcga cgaattctgc agatatcaca agtttgtaca aaaaagcagg cttcatggca
      121 gcgggggaga cgcagctcta cgccaaggtc tccaacaagc tcaagagccg cagcagcccc
      181 tcgctcctgg agcccctcct ggccatgggc ttcccggtgc acaccgcgct gaaagcgttg
      241 gcagccacgg ggaggaagac ggcggaggag gccttggcct ggctgcatga tcattgcaat
      301 gacccttccc tagacgaccc catcccccag gagtatgccc ttttcctctg tccaacgggg
      361 cccctgctgg aaaaacttca agagttctgg agagagagca agcgccagtg tgcaaagaac
      421 agagctcatg aggtcttccc acacgtgaca ctctgtgact tcttcacgtg tgaagaccag
      481 aaggtggaat gcctgtacga ggcgctgaag agagctggag acaggctcct gggctccttc
      541 cccacggccg tgcctctggc tctccactcc tccatcagct acctcggctt cttcgtcagt
      601 ggcagccccg cagacgtcat ccgggaattc gccatgacct tcgccacgga agcatctctc
      661 ttagcagact gctccgtgaa gccttgcacc aaacagctgc atctgacctt ggcccacaag
      721 ttctaccccc accaccagag gacgctggag cagctggcca gagccatccc cctgggccac
      781 agctgccagt ggaccgcagc actctactcc cgagacatgc gctttgtgca ctaccagacc
      841 ctgagagccc tattccagta caaaccccag aacgtggatg agctgacgct aagtcctggt
      901 gactacatct ttgtggaccc cacgcagcag gacgaagcca gcgagggctg ggtgattggg
      961 atctcacagc ggacgggctg ccggggcttc ctgccggaaa actacacgga tcgagccagt
     1021 gagtctgaca cgtgggtgaa gcacaggatg tacaccttca gtctagccac agacctgaac
     1081 tccagaaagg atggtgaagc cagcagcaga tgcagcgggg aatttcttcc acaaacggca
     1141 aggagtctta gcagcttaca ggccttgcag gctaccgttg caaggaagag tgtgctggtg
     1201 gttcgccacg gggagagagt ggatcagatc ttcgggaagg catggctgca gcaatgctcc
     1261 actcctgatg ggaaatacta caggccagac ctgaatttcc cctgcagtct gcccagacgg
     1321 agtcgtggga tcaaagactt tgaaaacgat cccccattat catcgtgtgg cattttccag
     1381 tccagaattg caggggacgc gctactggac agtggtatca gaatcagctc tgtgtttgcc
     1441 tccccagccc tccgctgtgt gcagacggcc aaactcatcc tggaagaact caaactggag
     1501 aaaaaaatca agatacgagt ggaacctgga atctttgaat ggacaaaatg ggaagctggc
     1561 aagaccaccc caaccctcat gagcctggaa gagctgaaag aggcaaattt caacattgac
     1621 actgattaca ggcccgcgtt tcccctgtcc gccctcatgc cggccgagag ctaccaggag
     1681 tacatggaca ggtgcacggc gagcatggtg caaatcgtca acacctgtcc gcaggacacg
     1741 ggtgtcatcc taattgtgag tcacggctcc actctggact cctgcacgcg gccactgctc
     1801 gggctgccgc cccgggaatg tggggatttt gcccaactcg tgagaaagat cccttccctg
     1861 ggcatgtgct tctgtgaaga aaataaagag gaaggaaaat gggagttggt gaacccaccg
     1921 gtgaagaccc tgacccacgg ggcgaacgca gcatttaact ggaggaactg gatctcaggc
     1981 aactgaaacc cagctttctt gtacaaagtg gtgataaacc cgctgatcag cctcgactgt
     2041 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2101 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2161 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2221 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     2281 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattna agcgcggcgg
     2341 gngtggnggt tacncgcanc gtgaccgcta cacttgccan cgccctagcg cccgctcctt
     2401 tcgcttttct tcccttcctt ctcgncacgt cgccggcttt cc
//
