LOCUS       Exported                2648 bp ds-DNA     linear   SYN 20-OCT-2022
DEFINITION  LR072513WTLgcVH_C1_T7 LR072513WTLgcVH_C1.0.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 20, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    4..28
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    complement(2015..2039)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             2065..2106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /note="color: #cc99b2"
                     /translation="GKPIPNPLLGLDST"
     CDS             2116..2133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     polyA_signal    2162..2386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 atcacaagtt tgtacaaaaa agcaggcttc atggcagcgg gggagacgca gctctacgcc
       61 aaggtctcca acaagctcaa gagccgcagc agcccctcgc ttctggagcc cctcctggcc
      121 atgggcttcc cggtgcacac cgcgctgaaa gcgttggcag ccacggggag gaagacggcg
      181 gaggaggcct tggcctggct gcatgatcat tgcaatgacc cttccctaga cgaccccatc
      241 ccccaggagt atgccctttt cctctgtcca acggggcccc tgctggaaaa acttcaagag
      301 ttctggagag agagcaagcg ccagtgtgca aagaacagag ctcatgaggt cttcccacac
      361 gtgacactct gtgacttctt cacgtgtgaa gaccagaagg tggaatgcct gtacgaggcg
      421 ctgaagagag ctggagacag gctcctgggc tccttcccca cggccgtgcc tctggctctc
      481 cactcctcca tcagctacct cggcttcttc gtcagtggca gccccgcaga cgtcatccgg
      541 gaattcgcca tgaccttcgc cacggaagca tctctcttag caggcacttc cgtttcccgc
      601 ttctggattt tcagccaggt gcctggacat ggccctaacc tgaggctgag caatttaact
      661 agagcctcct tcgtgagcca ctacatcctt caaaaatact gctccgtgaa gccttgcacc
      721 aaacagctgc atctgacctt ggcccacaag ttctaccccc accaccagag gacgctggag
      781 cagctggcca gagccatccc cctgggccac agctgccagt ggaccgcagc actctactcc
      841 cgagacatgc gctttgtgca ctaccagacc ctgagagccc tattccagta caaaccccag
      901 aacgtggatg agctgacgct aagtcctggt gactacatct ttgtggaccc cacgcagcag
      961 gacgaagcca gcgagggctg ggtgattggg atctcacagc ggacgggctg ccggggcttc
     1021 ctgccggaaa actacacgga tcgagccagt gagtctgaca cgtgggtgaa gcacaggatg
     1081 tacaccttca gtctagccac agacctgaac tccagaaagg atggtgaagc cagcagcaga
     1141 tgcagcgggg aatttcttcc acaaacggca aggagtctta gcagcttaca ggccttgcag
     1201 gctaccgttg caaggaagag tgtgctggtg gttcgccacg gggagagagt ggatcagatc
     1261 ttcgggaagg catggctgca gcaatgctcc actcctgatg ggaaatacta caggccagac
     1321 ctgaatttcc cctgcagtct gcccaggcgg agtcgtggga tcaaagactt tgaaaacgat
     1381 cccccattat catcgtgtgg cattttccag tccagaattg caggggacgc gctactggac
     1441 agtggtatca gaatcagctc tgtgtttgcc tccccagccc tccgctgtgt gcagacggcc
     1501 aaactcatcc tggaagaact caaactggag aaaaaaatca agatacgagt ggaacctgga
     1561 atctttgaat ggacaaaatg ggaagctggc aaaaccaccc caaccctcat gagcctggaa
     1621 gagctgaaag aggcaaattt caacattgac actgattaca ggcccgcgtt tcccctgtcc
     1681 gccctcatgc cggccgagag ctaccaggag tacatggaca ggtgcacggc gagcatggtg
     1741 caaatcgtca acacctgccc gcaggacacg ggtgtcatcc taattgtgag tcacggctcc
     1801 actctggact cctgcacgcg gccactgctc gggctgccgc cccgggaatg tggggatttt
     1861 gcccaactcg tgagaaagat cccttccctg ggcatgtgct tctgtgaaga aaataaagag
     1921 gaaggaaaat gggagttggt gaacccaccg gtgaagaccc tgacccacgg ggcgaacgca
     1981 gcatttaact ggaggaactg gatctcaggc aacaacccag ctttcttgta caaagtggtt
     2041 gatctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     2101 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     2161 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2221 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2281 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2341 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     2401 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     2461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2581 aatcgggggc tccctttagg gttccgattt agtgcttacg gcacctcnac cccaaaaact
     2641 tgattagg
//
