LOCUS       Exported                2280 bp ds-DNA     linear   SYN 20-OCT-2022
DEFINITION  Lig080414_C2_pGexF Lig080414_C2.0.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 20, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             17..40
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /note="color: #cc99b2
                     Cleavage site after base 34"
                     /translation="LEVLFQGP"
ORIGIN
        1 tcctccnaaa tcggatctgg aagttctgtt ccaggggccc ctgggatcca tggcagcggg
       61 ggagacgcag ctctacgcca aggtctccaa caagctcaag agccgcagca gcccctcgct
      121 cctggagccc ctcctggcca tgggcttccc ggtgcacacc gcgctgaaag cgttggcagc
      181 cacggggagg aagacggcgg aggaggcctt ggcctggctg catgatcatt gcaatgaccc
      241 ttccctagac gaccccatcc cccaggagta tgcccttttc ctctgtccaa cggggcccct
      301 gctggaaaaa cttcaagagt tctggagaga gagcaagcgc cagtgtgcaa agaacagagc
      361 tcatgaggtc ttcccacacg tgacactctg tgacttcttc acgtgtgaag accagaaggt
      421 ggaatgcctg tacgaggcgc tgaagagagc tggagacagg ctcctgggct ccttccccac
      481 ggccgtgcct ctggctctcc actcctccat cagctacctc ggcttcttcg tcagtggcag
      541 ccccgcagac gtcatccggg aattcgccat gaccttcgcc acggaagcat ctctcttagc
      601 agactgctcc gtgaagcctt gcaccaaaca gctgcatctg accttggccc acaagttcta
      661 cccccaccac cagaggacgc tggagcagct ggccagagcc atccccctgg gccacagctg
      721 ccagtggacc gcagcactct actcccgaga catgcgcttt gtgcactacc agaccctgag
      781 agccctattc cagtacaaac cccagaacgt ggatgagctg acgctaagtc ctggtgacta
      841 catctttgtg gaccccacgc agcaggacga agccagcgag ggctgggtga ttgggatctc
      901 acagcggacg ggctgccggg gcttcctgcc ggaaaactac acggatcgag ccagtgagtc
      961 tgacacgtgg gtgaagcaca ggatgtacac cttcagtcta gccacagacc tgaactccag
     1021 aaaggatggt gaagccagca gcagatgcag cggggaattt cttccacaaa cggcaaggag
     1081 tcttagcagc ttacaggcct tgcaggctac cgttgcaagg aagagtgtgc tggtggttcg
     1141 ccacggggag agagtggatc agatcttcgg gaaggcatgg ctgcagcaat gctccactcc
     1201 tgatgggaaa tactacaggc cagacctgaa tttcccctgc agtctgccca gacggagtcg
     1261 tgggatcaaa gactttgaaa acgatccccc attatcatcg tgtggcattt tccagtccag
     1321 aattgcaggg gacgcgctac tggacagtgg tatcagaatc agctctgtgt ttgcctcccc
     1381 agccctccgc tgtgtgcaga cggccaaact catcctggaa gaactcaaac tggagaaaaa
     1441 aatcaagata cgagtggaac ctggaatctt tgaatggaca aaatgggaag ctggcaagac
     1501 caccccaacc ctcatgagcc tggaagagct gaaagaggca aatttcaaca ttgacactga
     1561 ttacaggccc gcgtttcccc tgtccgccct catgccggcc gagagctacc aggagtacat
     1621 ggacaggtgc acggcgagca tggtgcaaat cgtcaacacc tgtccgcagg acacgggtgt
     1681 catcctaatt gtgagtcacg gctccactct ggactcctgc acgcggccac tgctcgggct
     1741 gccgccccgg gaatgtgggg attttgccca actcgtgaga aagatccctt ccctgggcat
     1801 gtgcttctgt gaagaaaata aagaggaagg aaaatgggag ttggtgaacc caccggtgaa
     1861 gaccctgacc cacggggcga acgcagcatt taactggagg aactggatct caggcaactg
     1921 actcgagcgg ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg gtgatgacgg
     1981 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     2041 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     2101 catgacccag tcacgtagcg atagncggag tgtatanatt cttgaagacg nnaggggcct
     2161 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2221 tggcactttt cggggaaatg tgcncggaac ccctaatttg ntaatttttc taaatacatt
//
