LOCUS       pJH2633                 8411 bp    DNA        circular     30-AUG-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      7350..8032
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    8372..8394
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     35..54
                     /locus_tag="A primer"
                     /label="A primer"
                     /ApEinfo_label="A primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6593..7252
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     UTR             5187..5921
                     /locus_tag="unc-54 3UTR"
                     /label="unc-54 3UTR"
                     /ApEinfo_label="unc-54 3UTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2844..2864)
                     /locus_tag="B primer"
                     /label="B primer"
                     /ApEinfo_label="B primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     exon            5670..5824
                     /locus_tag="aex-5 exon1"
                     /label="aex-5 exon1"
                     /ApEinfo_label="aex-5 exon1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    join(4339..4341,4348..5058)
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0f18"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3111..3111
                     /locus_tag="Tetanus Toxin Light Chain"
                     /label="Tetanus Toxin Light Chain"
                     /ApEinfo_label="Tetanus Toxin Light Chain"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2950..4322
                     /locus_tag="Tetanus Toxin Light Chain(1)"
                     /label="Tetanus Toxin Light Chain(1)"
                     /ApEinfo_label="Tetanus Toxin Light Chain"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        35..2864
                     /locus_tag="psra-11"
                     /label="psra-11"
                     /ApEinfo_label="psra-11"
                     /ApEinfo_fwdcolor="#fdceff"
                     /ApEinfo_revcolor="#fdceff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ATGACCATGA TTACGCCAAG CTTGCATGCG CGGCcgcacg ttttcccttt acattagcaa
       61 aaccgaccaa cgaccgaaac ctaagcatta tccaggccta aacatatttt aaaaaaagtg
      121 tagtttacca aatgggagac attttccttg ttctttgtgg gatgcttttg aaattgaata
      181 gaaatctcta gattaatcaa tgtgtttgct ccgatgcgcc tagtctcaaa gccttctcat
      241 gttgcaaaca aatgcactaa ctaaactaat taacaagtca cggaggagat tcaaaacatt
      301 tcacttttca ggaaagtagc atttttaaat atattcatgt tgtagatttg tcattttgca
      361 atatttttta tcaaaattgg attacaccct cttttgagca gtctgacttg ttggctgact
      421 tgattttctt ttaaagtgat gaaattttat tcatatttga tttttagtaa ataaattatt
      481 gcaaacccca ttatgcaact ttgacagctt atatctcagt tgccgtttgt tttatgaaaa
      541 attgtcaact taccaaataa ttcttagtta cgatctgaaa tttttcgaaa actctaatct
      601 ttagtcaaaa atgttcagca atgtaaacat tttttcatta caaaaaaaga tgatgtagat
      661 gcatacatca agtatctcaa ccggaacacc ccctattttg caccatctct gtcatctttt
      721 tcttcctttt tcagatggga aacatacaat taatgctcac atgtcatgtc tagtcatttg
      781 tttttttttg tttgttttta atccgaatct cttattcatc tttagacgtt ccattcggca
      841 gaggcgagtt ggggcgcctc ttactcgcct caaattttgt gtttgcactc ataatcaaag
      901 aataaattat cttgcccatt ttggtccttg accatttgtg taggcaatag gcaggcagac
      961 atattttctt aaacagaaga ccgaactgtg agtgtacaaa aataaattaa atattaccta
     1021 cctttgttag aatttggaat aagattaaaa aattcgttgt cctctgtgga attgctcatt
     1081 attagatttt tacgaaatca tgcggattag aaagtttaat atttatgtac agtgcgtcca
     1141 acttctatag cacccccctc caattcggcc gtttggagct tttcaaaata tttttcggaa
     1201 aagtgttagt gcagttcgat agcaaatatt gaaaaaccta tgctccccaa tttttatcat
     1261 gatatctcaa aaatcacgga aattgggtca aaatatctga aaaatggccg tcgaacttct
     1321 atagcacccc ctctgatttt tgacaaattt ctgtaaattc ggggttttct tcgtagttaa
     1381 tgaatttatt ttgtaaataa cttcacacca aaataatcag gtggtttcac tttcacattt
     1441 aaaaaataaa tataacactt gaaaacacgt ggaatgcctg ggattgatca aaaaattgat
     1501 caaaatgttc ttctccgtct attcattact atgtaattag ttgtagaaag catttattca
     1561 tatgaaccaa atggtaaagg gagcaacttc agtggttttt aaaaggctcg atttaaccat
     1621 gaaaacgagc gttcggaggc acaaagctga aggttttgac ttcgtattgt acagaacttc
     1681 agctcatcct gctcaaatgt ctttgaagct tcgattttga ttagatgtct ttgaaaattt
     1741 atacggaaaa gatcatgaga ccgctcaaaa agaggacaaa ggcaaagcga ggccgcaagt
     1801 ctttttttca agctaacatg cgaaaatttt agacagaaaa gtaagatata cctttccaaa
     1861 tgacgatttt gctagttgta tttataactc aagttaaatt tagcgaattc gctcattgaa
     1921 tctttttttg gtcaagtaat gcaaaagcta ccagacaaaa cattgaacct gcgattgtag
     1981 atgtctggca tctgatttac ccatggattt ccacggattt tttgttcata taaaataatt
     2041 tttaattttt gctataatgt agtaattttt agtcgaaaat gtattcccaa ccattatctc
     2101 tgcgcctccg aacgctcgtt ttcatggtta aatcgaacct tttaaaaacc actgaagttg
     2161 ctccctttac catttggttc atatgaataa atgctttcta caactaatta tatagtaatg
     2221 aatagacgga gaagaacatt ttgatctatt ttggtgctgc gaaaatcatt gggagccaag
     2281 atatggcctc tgaagcaaat aagaacgttg aggggggtgc tatagaagtt ggacgcactg
     2341 tatttccaat tattttttaa gaatttcgat tttataaaca gcctttgctc cgcctatttg
     2401 agctcatgat gtttctcttt tgttttcaag attgttgatt ccttcctatt gtcctattgt
     2461 gatttttaaa tttttaaatt gctcattaac ttgattttta tttagtacat atctctaata
     2521 tgttgtttca cattgcacca accggtctca atttttgaaa ttatttattg ttatgtttga
     2581 atattttaaa caaatttaaa aatttagaaa aattgctgag ctgttgccta aactcataag
     2641 tgtttcaaaa tttgctacga ttttgcatgt actttttaat attttatatg ctttttcgta
     2701 ttccaaaaca aacaatttag tttactttac aaattatgaa caaaaaaaat ttgatgtaac
     2761 ggagagaaaa ttattaaacg ttacatgtat tttttgatcg tatcgagaaa aatgcaaaaa
     2821 atatcaaatt gtaatttttc cgcacatctc cgcaacacac aattcCCGGG ATTGGCCAAA
     2881 GGACCCAAAG GTATGTTTCG AATGATACTA ACATAACATA GAACATTTTC AGGAGGACCC
     2941 TTGGCTAGCa tgccgatcac catcaacaac ttccgttact ccgacccggt taacaacgac
     3001 accatcatca tgatggaacc gccgtactgc aaaggtctag acatctacta caaagccttc
     3061 aaaatcaccg accgtatctg gatcgttccg gaacgttacg aatttggtac Taaaccggaa
     3121 gacttcaacc cgccgtcctc cctgatcgaa ggtgcttccg aatactacga cccgaactac
     3181 ctgcgtaccg actccgacaa agaccgtttc ctgcagacca tggttaaact gttcaaccgt
     3241 atcaaaaaca acgttgctgg tgaagctctg ctggacaaaa tcatcaacgc tatcccgtac
     3301 ctgggtaact cctactccct gctggacaaa ttcgacacca actccaactc cgtttccttc
     3361 aacctgctgg aacaggatcc gtccggtgct accaccaaat ccgctatgct gaccaacctg
     3421 atcatcttcg gtccgggtcc ggttctgaac aaaaacgaag ttcgtggtat cgttctgcgt
     3481 gttgacaaca aaaactactt cccgtgccgt gacggtttcg gttccatcat gcagatggct
     3541 ttctgcccgg aatacgtacc gaccttcgac aacgttatcg aaaacatcac ctccctgacc
     3601 atcggtaaat ccaaatactt ccaggacccg gctctgctgc tgatgcacga actgatccac
     3661 gttctgcacg gtctgtacgg catgcaggtt tcctcccacg aaatcatccc gtccaaacag
     3721 gaaatctaca tgcagcacac ctacccgatc tccgctgaag agctcttcac cttcggtggt
     3781 caggacgcta acctgatctc catcgatatc aaaaacgacc tgtacgaaaa aaccctgaac
     3841 gactacaaag ctatcgctaa caaactgtcc caggttacct cctgcaacga cccgaacatc
     3901 gacatcgact cctacaaaca gatctatcag cagaagtatc agtttgataa ggattccaac
     3961 ggccagtata tagtgaacga ggataagttt cagatactgt ataactccat aatgtatggc
     4021 tttactgaga tagagctggg caagaagttt aacataaaga ctcgcttaag ctatttttcc
     4081 atgaaccacg atcctgtgaa gatacctaac ctgctggatg atactatata taacgatact
     4141 gagggcttta acatagagtc caaggatctg aagtccgagt ataagggcca gaacatgagg
     4201 gtgaacacta acgcctttag gaacgtcgac ggttccggtc tggtttccaa actgatcggt
     4261 ctgtgcaaaa aaatcatccc gccgaccaac atccgtgaaa acctgtacaa ccgtaccgct
     4321 agGGTACCgg tagaaaaaAT GctcgagATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC
     4381 ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG
     4441 TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG
     4501 AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG
     4561 TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC
     4621 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC
     4681 GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC
     4741 ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC
     4801 TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG
     4861 CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC
     4921 GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG
     4981 GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG
     5041 GACGAGCTGT ACAAGTAAGA ATTCcaactg agcgccggtc gctaccatta ccaacttgtc
     5101 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     5161 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     5221 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     5281 attatcaaaa aaacttcttc ttaatttctt tgttttttag cttcttttaa gtcacctcta
     5341 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     5401 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     5461 cacttcttat gtttttttta cttctgataa attttttttg aaacatcata gaaaaaaccg
     5521 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     5581 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     5641 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     5701 ggggtttccc ctattgtttg tcaagagttt cgaggacggc gtttttcttg ctaaaatcac
     5761 aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg
     5821 gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta
     5881 aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg
     5941 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6001 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6061 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     6121 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6181 gcatcaggcg gccttaatta agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6241 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6361 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6421 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6481 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6541 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6601 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6661 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6721 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6781 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6841 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6901 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6961 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7021 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7081 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7141 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7201 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7261 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7321 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7381 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7441 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7501 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7561 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7621 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7681 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7741 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7801 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7861 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7921 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7981 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8041 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8101 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8161 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8221 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8281 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8341 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8401 aggaaacagc t    
//
