LOCUS       Exported                4675 bp ds-DNA     circular SYN 26-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   gaosiqi
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          750..761
                     /organism="Helicobacter sp. MIT 11-5569"
                     /host="mouse"
                     /strain="MIT 11-5569"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /isolation_source="feces"
                     /collection_date=2011
                     /db_xref="taxon:1548151"
     rep_origin      7..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     641..662
                     /label=primer2
     primer_bind     643..661
                     /label=Primer 1
     misc_feature    657..672
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     complement(666..715)
                     /label=TV0-tracr-T4-R
     misc_feature    complement(677..1095)
                     /label=U6+gRNA
     misc_feature    complement(684..761)
                     /label=Hsp1-sgRNA scaffold
     primer_bind     731..782
                     /label=TV0-tracr-T4-F
     misc_feature    complement(762..763)
                     /label=U6+gRNA
     misc_feature    complement(762..763)
                     /label=U6+gRNA
     misc_feature    complement(786..1095)
                     /label=mU6
     primer_bind     complement(1088..1107)
                     /label=Primer 3
     misc_feature    1131..1135
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1131..1132
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1136..1466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1200..1456
                     /label=SV40 promoter
     CDS             1476..2072
                     /codon_start=1
                     /label=puro
                     /label=puro(R)
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     polyA_signal    2288..2422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2288..2418
                     /label=SV40 PolyA
     misc_feature    2423..2477
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     complement(2437..2453)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2476..2493)
                     /label=Primer 2
     promoter        complement(2490..2508)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2529..2545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2553..2569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2577..2607)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(2931..3519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3690..4550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4551..4655)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccgggccccc cctcgagact agtaaaaaat cggttttaag ggattgtaac cctgcagaga
      721 ccgaaaactc cttattttag ccacttttca gtggctaaaa cacgtcttct gagaagactg
      781 caaacaaggc ttttctccaa gggatattta tagtctcaaa acacacaatt actttacagt
      841 tagggtgagt ttccttttgt gctgtttttt aaaataataa tttagtattt gtatctctta
      901 tagaaatcca agcctatcat gtaaaatgta gctagtatta aaaagaacag attatctgtc
      961 ttttatcgca cattaagcct ctatagttac taggaaatat tatatgcaaa ttaaccgggg
     1021 caggggagta gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc gggcctagag
     1081 atggcgcgtc ggatctctag agcgggcccg gtactaacca aactggatat cgatagtgtg
     1141 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     1201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     1321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     1381 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     1441 tttttggagg cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc
     1501 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     1561 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     1621 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     1681 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     1741 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     1801 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     1861 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     1921 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     1981 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg
     2041 cgacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     2101 cgaccgaaag gagcgcacga ccccatgcat cgaaatgacc gaccaagcga cgcccaacct
     2161 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     2221 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     2281 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2401 tgtatcttat catgtctgga tccctgcagc ccgggggatc cactagttct agagcggccg
     2461 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     2521 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2581 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2761 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2821 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3361 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3421 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3481 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3541 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3601 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3661 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4561 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgc
//
