LOCUS       Exported                5196 bp ds-DNA     circular SYN 26-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 26, 2022 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     
            20-MAY-2010
            SOURCE      
              ORGANISM  
            COMMENT     VNTDATE|538328968|
            COMMENT     VNTDBDATE|538763081|
            COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
            COMMENT     VNTAUTHORNAME|William Hendriks|
            COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
            COMMENT     VNTAUTHORNAME|Feng Zhang|
            COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            1..141
                     /label=L-ITR
     misc_feature    132..1315
                     /label=gBlock_LY05A
                     /note="gBlock_LY05A"
     misc_feature    132..161
                     /label=gBlock arm L
                     /note="gBlock arm L"
     misc_feature    167..393
                     /label=SB IR/DR(L)
                     /note="SB IR/DR(L)"
     misc_feature    460..708
                     /label=U6 promoter
                     /note="U6 promoter"
     misc_feature    709..726
                     /label=BbsI
                     /note="BbsI"
     misc_feature    727..808
                     /label=chimeric RNA backbone
                     /note="chimeric RNA backbone "
     misc_feature    830..853
                     /label=pLY05_R1
                     /note="pLY05_R1"
     misc_feature    837..1092
                     /label=EFS-NS
                     /note="EFS-NS"
     misc_feature    1099..2121
                     /label=SB100X
                     /note="SB100X"
     misc_feature    1238..1258
                     /label=pLY05_F1
                     /note="pLY05_F1"
     misc_feature    1286..2463
                     /label=gBlock_LY05B
                     /note="gBlock_LY05B"
     misc_feature    1505..1526
                     /label=pLY05_R2
                     /note="pLY05_R2"
     misc_feature    1816..1818
                     /label=GTC&gt;GTT(BbsI mutation)
                     /note="GTC>GTT(BbsI mutation)"
     misc_feature    1896..1916
                     /label=pLY05_F2
                     /note="pLY05_F2"
     misc_feature    2138..2185
                     /label=Short PA
                     /note="Short PA"
     misc_feature    2192..2419
                     /label=SB IR/DR(R)
                     /note="SB IR/DR(R)"
     misc_feature    2432..2463
                     /label=gBlock arm R
                     /note="gBlock arm R"
     gene            2459..2599
                     /label=R-ITR
     exon            2691..2997
                     /label=f1 Origin
     exon            3516..4373
                     /label=AmpR
     rep_origin      4524..5191
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttctag actatacagt tgaagtcgga
      181 agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact ccacaaattt
      241 cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt gcatgacaca
      301 agtcattttt ccaacaattg tttacagaca gattatttca cttataattc actgtatcac
      361 aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa acagcttgga
      421 aaattccaga aaatgatgtc atggctttag agaggatccg agggcctatt tcccatgatt
      481 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
      541 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      601 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      661 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gtcttcgaga
      721 agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      781 aaaagtggca ccgagtcggt gcttttttgg ctagctggcc gcgtttaaac gtcgactagg
      841 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      901 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
      961 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     1021 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     1081 ccagaacaca ggctcgagat gggaaaatca aaagaaatca gccaagacct cagaaaaaga
     1141 attgtagacc tccacaagtc tggttcatcc ttgggagcaa tttccaaacg cctggcggta
     1201 ccacgttcat ctgtacaaac aatagtacgc aagtataaac accatgggac cacgcagccg
     1261 tcataccgct caggaaggag acgcgttctg tctcctagag atgaacgtac tttggtgcga
     1321 aaagtgcaaa tcaatcccag aacaacagca aaggaccttg tgaagatgct ggaggaaaca
     1381 ggtacaaaag tatctatatc cacagtaaaa cgagtcctat atcgacataa cctgaaaggc
     1441 cactcagcaa ggaagaagcc actgctccaa aaccgacata agaaagccag actacggttt
     1501 gcaactgcac atggggacaa agatcgtact ttttggagaa atgtcctctg gtctgatgaa
     1561 acaaaaatag aactgtttgg ccataatgac catcgttatg tttggaggaa gaagggggag
     1621 gcttgcaagc cgaagaacac catcccaacc gtgaagcacg ggggtggcag catcatgttg
     1681 tgggggtgct ttgctgcagg agggactggt gcacttcaca aaatagatgg catcatggac
     1741 gcggtgcagt atgtggatat attgaagcaa catctcaaga catcagtcag gaagttaaag
     1801 cttggtcgca aatgggtttt ccaacacgac aatgacccca agcatacttc caaagttgtg
     1861 gcaaaatggc ttaaggacaa caaagtcaag gtattggagt ggccatcaca aagccctgac
     1921 ctcaatccta tagaaaattt gtgggcagaa ctgaaaaagc gtgtgcgagc aaggaggcct
     1981 acaaacctga ctcagttaca ccagctctgt caggaggaat gggccaaaat tcacccaaat
     2041 tattgtggga agcttgtgga aggctacccg aaacgtttga cccaagttaa acaatttaaa
     2101 ggcaatgcta ccaaatacta ggggccctaa ccgcgggaat aaaagatctt tattttcatt
     2161 agatctgtgt gttggttttt tgtgtgaatt cttgagtgta tgtaaacttc tgacccactg
     2221 ggaatgtgat gaaagaaata aaagctgaaa tgaatcattc tctctactat tattctgata
     2281 tttcacattc ttaaaataaa gtggtgatcc taactgacct aagacaggga atttttacta
     2341 ggattaaatg tcaggaattg tgaaaaagtg agtttaaatg tatttggcta aggtgtatgt
     2401 aaacttccga cttcaactgt ataggcatgc ggtaaccacg tgcggaccga gcggccgcag
     2461 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     2521 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     2581 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2641 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     2701 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2761 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2821 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2881 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2941 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3001 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3061 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     3121 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3181 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3241 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3301 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3361 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3481 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3541 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3601 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3661 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3721 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3781 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3841 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3901 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3961 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4021 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4081 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4141 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4201 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4261 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4321 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4381 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4441 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4681 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5161 ggttcctggc cttttgctgg ccttttgctc acatgt
//
