LOCUS       Exported                5745 bp ds-DNA     circular SYN 26-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 26, 2022 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     
            20-MAY-2010
            SOURCE      
              ORGANISM  
            COMMENT     VNTDATE|538328968|
            COMMENT     VNTDBDATE|538763081|
            COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
            COMMENT     VNTAUTHORNAME|William Hendriks|
            COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
            COMMENT     VNTAUTHORNAME|Feng Zhang|
            COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            1..141
                     /label=L-ITR
     misc_feature    132..161
                     /label=gBlock arm L
                     /note="gBlock arm L"
     misc_feature    167..393
                     /label=SB IR/DR(L)
                     /note="SB IR/DR(L)"
     misc_feature    460..708
                     /label=U6 promoter
                     /note="U6 promoter"
     misc_feature    709..726
                     /label=BbsI
                     /note="BbsI"
     misc_feature    727..808
                     /label=chimeric RNA backbone
                     /note="chimeric RNA backbone "
     misc_feature    830..853
                     /label=pLY05_R1
                     /note="pLY05_R1"
     misc_feature    837..1092
                     /label=EFS-NS
                     /note="EFS-NS"
     misc_feature    1069..1120
                     /label=pLY017-TyF1
                     /note="pLY017-TyF1"
     misc_feature    1099..1584
                     /label=Thy1.1 mouse
                     /note="Thy1.1 mouse"
     misc_feature    1423..1425
                     /label=GTC&gt;GTG
                     /note="GTC>GTG"
     misc_feature    1585..1647
                     /label=T2A
                     /note="T2A"
     misc_feature    1624..1672
                     /label=pLY017-R1
                     /note="pLY017-R1"
     misc_feature    1648..2670
                     /label=SB100X
                     /note="SB100X"
     misc_feature    1787..1807
                     /label=pLY05_F1
                     /note="pLY05_F1"
     misc_feature    1835..3012
                     /label=gBlock_LY05B
                     /note="gBlock_LY05B"
     misc_feature    2054..2075
                     /label=pLY05_R2
                     /note="pLY05_R2"
     misc_feature    2365..2367
                     /label=GTC&gt;GTT(BbsI mutation)
                     /note="GTC>GTT(BbsI mutation)"
     misc_feature    2445..2465
                     /label=pLY05_F2
                     /note="pLY05_F2"
     misc_feature    2687..2734
                     /label=Short PA
                     /note="Short PA"
     misc_feature    2741..2968
                     /label=SB IR/DR(R)
                     /note="SB IR/DR(R)"
     misc_feature    2981..3012
                     /label=gBlock arm R
                     /note="gBlock arm R"
     gene            3008..3148
                     /label=R-ITR
     exon            3240..3546
                     /label=f1 Origin
     exon            4065..4922
                     /label=AmpR
     rep_origin      5073..5740
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttctag actatacagt tgaagtcgga
      181 agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact ccacaaattt
      241 cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt gcatgacaca
      301 agtcattttt ccaacaattg tttacagaca gattatttca cttataattc actgtatcac
      361 aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa acagcttgga
      421 aaattccaga aaatgatgtc atggctttag agaggatccg agggcctatt tcccatgatt
      481 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
      541 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      601 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      661 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gtcttcgaga
      721 agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      781 aaaagtggca ccgagtcggt gcttttttgg ctagctggcc gcgtttaaac gtcgactagg
      841 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      901 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
      961 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     1021 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     1081 ccagaacaca ggctcgagat gaacccagcc atcagcgtcg ctctcctgct ctcagtcttg
     1141 caggtgtccc gagggcagaa ggtgaccagc ctgacagcct gcctggtgaa ccaaaacctt
     1201 cgcctggact gccgccatga gaataacacc aaggataact ccatccagca tgagttcagc
     1261 ctgacccgag agaagaggaa gcacgtgctc tcaggcaccc ttgggatacc cgagcacacg
     1321 taccgctccc gcgtcaccct ctccaaccag ccctatatca aggtccttac cctagccaac
     1381 ttcaccacca aggatgaggg cgactacttt tgtgagcttc gcgtgtcggg cgcgaatccc
     1441 atgagctcca ataaaagtat cagtgtgtat agagacaagc tggtcaagtg tggcggcata
     1501 agcctgctgg ttcagaacac atcctggatg ctgctgctgc tgctttccct ctccctcctc
     1561 caagccctgg acttcatttc tctgggcagt ggagagggca gaggaagtct gctaacatgc
     1621 ggtgacgtcg aggagaatcc tggcccaatg ggaaaatcaa aagaaatcag ccaagacctc
     1681 agaaaaagaa ttgtagacct ccacaagtct ggttcatcct tgggagcaat ttccaaacgc
     1741 ctggcggtac cacgttcatc tgtacaaaca atagtacgca agtataaaca ccatgggacc
     1801 acgcagccgt cataccgctc aggaaggaga cgcgttctgt ctcctagaga tgaacgtact
     1861 ttggtgcgaa aagtgcaaat caatcccaga acaacagcaa aggaccttgt gaagatgctg
     1921 gaggaaacag gtacaaaagt atctatatcc acagtaaaac gagtcctata tcgacataac
     1981 ctgaaaggcc actcagcaag gaagaagcca ctgctccaaa accgacataa gaaagccaga
     2041 ctacggtttg caactgcaca tggggacaaa gatcgtactt tttggagaaa tgtcctctgg
     2101 tctgatgaaa caaaaataga actgtttggc cataatgacc atcgttatgt ttggaggaag
     2161 aagggggagg cttgcaagcc gaagaacacc atcccaaccg tgaagcacgg gggtggcagc
     2221 atcatgttgt gggggtgctt tgctgcagga gggactggtg cacttcacaa aatagatggc
     2281 atcatggacg cggtgcagta tgtggatata ttgaagcaac atctcaagac atcagtcagg
     2341 aagttaaagc ttggtcgcaa atgggttttc caacacgaca atgaccccaa gcatacttcc
     2401 aaagttgtgg caaaatggct taaggacaac aaagtcaagg tattggagtg gccatcacaa
     2461 agccctgacc tcaatcctat agaaaatttg tgggcagaac tgaaaaagcg tgtgcgagca
     2521 aggaggccta caaacctgac tcagttacac cagctctgtc aggaggaatg ggccaaaatt
     2581 cacccaaatt attgtgggaa gcttgtggaa ggctacccga aacgtttgac ccaagttaaa
     2641 caatttaaag gcaatgctac caaatactag gggccctaac cgcgggaata aaagatcttt
     2701 attttcatta gatctgtgtg ttggtttttt gtgtgaattc ttgagtgtat gtaaacttct
     2761 gacccactgg gaatgtgatg aaagaaataa aagctgaaat gaatcattct ctctactatt
     2821 attctgatat ttcacattct taaaataaag tggtgatcct aactgaccta agacagggaa
     2881 tttttactag gattaaatgt caggaattgt gaaaaagtga gtttaaatgt atttggctaa
     2941 ggtgtatgta aacttccgac ttcaactgta taggcatgcg gtaaccacgt gcggaccgag
     3001 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3061 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3121 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     3181 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3241 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3301 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3361 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3421 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3481 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3541 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     3601 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3661 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3721 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3781 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3841 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     3901 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3961 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4021 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4081 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4141 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4201 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4261 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4321 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4381 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4441 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4501 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4561 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4621 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4681 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4741 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4801 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4861 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4921 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4981 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5041 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5101 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5161 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5221 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5281 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5341 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5401 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5461 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5521 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5581 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5641 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5701 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
