LOCUS       Exported                4382 bp DNA     circular SYN 16-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
COMMENT     LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     
            20-MAY-2010

            SOURCE      

              ORGANISM  

            COMMENT     VNTDATE|538328968|

            COMMENT     VNTDBDATE|538763081|

            COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|

            COMMENT     VNTAUTHORNAME|William Hendriks|

            COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|

            COMMENT     VNTAUTHORNAME|Feng Zhang|

            COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     source          1..4382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..141
                     /label=L-ITR
     misc_feature    150..155
                     /label=MluI
                     /note="MluI"
     promoter        162..411
                     /label=Human U6 Promoter
     misc_feature    162..187
                     /label=misc_feature 1
     misc_feature    378..412
                     /label=Readout_priming_site_long_F
                     /note="Readout_priming_site_long_F"
     misc_feature    389..412
                     /label=Readout_priming_site_F
                     /note="Readout_priming_site_F"
     misc_feature    393..411
                     /label=misc_feature 2
     misc_feature    412
                     /label=chimeric RNA backbone 1
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    413..433
                     /label=DR of LbCpf1
                     /note="DR of LbCpf1"
     misc_feature    434..449
                     /label=Double BbsI
                     /note="Double BbsI"
     misc_feature    456..496
                     /label=arrayRLBC1
                     /note="arrayRLBC1"
     misc_feature    503..758
                     /label=EFS-NS
                     /note="EFS-NS"
     misc_feature    783..1499
                     /label=GFP
                     /note="GFP"
     misc_feature    1500..1547
                     /label=NLS
                     /note="NLS"
     misc_feature    1569..1616
                     /label=shortPA
                     /note="shortPA"
     gene            1645..1785
                     /label=R-ITR
     exon            1877..2183
                     /label=f1 Origin
     exon            2702..3559
                     /label=AmpR
     rep_origin      3710..4377
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttctag aagagggcct atttcccatg
      181 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
      241 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
      301 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
      361 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtaatttct
      421 actaagtgta gatggtcttc gagaagacct tttttaagct tggcgtggat ccgatatcaa
      481 ctagatcttg agacaaggta cctaggtctt gaaaggagtg ggaattggct ccggtgcccg
      541 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      601 ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      661 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      721 cgttcttttt cgcaacgggt ttgccgccag aacacaggac cggttctaga cgtacggcca
      781 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      841 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      901 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      961 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1021 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1081 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1141 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1201 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1261 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1321 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1381 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     1441 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     1501 agcgtcctgc tgctactaag aaagctggtc aagctaagaa aaagaaataa gaattcgata
     1561 tcaagcttaa taaaagatct ttattttcat tagatctgtg tgttggtttt ttgtgtggta
     1621 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     1681 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     1741 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     1801 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     1861 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1921 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1981 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2041 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     2101 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2161 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     2221 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2281 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     2341 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     2401 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     2461 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     2521 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2701 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2761 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2821 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2881 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2941 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3001 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3061 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3121 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3181 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3241 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3301 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3361 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3421 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3481 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3541 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3601 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3661 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3721 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3781 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3841 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3901 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3961 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4021 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4081 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4141 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4201 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4261 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4321 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4381 gt
//
