LOCUS       Exported                4448 bp DNA     circular SYN 16-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
COMMENT     LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     
            20-MAY-2010

            SOURCE      

              ORGANISM  

            COMMENT     VNTDATE|538328968|

            COMMENT     VNTDBDATE|538763081|

            COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|

            COMMENT     VNTAUTHORNAME|William Hendriks|

            COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|

            COMMENT     VNTAUTHORNAME|Feng Zhang|

            COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     source          1..4448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..141
                     /label=L-ITR
     misc_feature    150..155
                     /label=MluI
                     /note="MluI"
     promoter        162..411
                     /label=Human U6 Promoter
     misc_feature    162..187
                     /label=misc_feature 1
     misc_feature    378..412
                     /label=Readout_priming_site_long_F
                     /note="Readout_priming_site_long_F"
     misc_feature    389..412
                     /label=Readout_priming_site_F
                     /note="Readout_priming_site_F"
     misc_feature    393..411
                     /label=misc_feature 2
     misc_feature    412
                     /label=chimeric RNA backbone 1
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    413..433
                     /label=DR of LbCpf1
                     /note="DR of LbCpf1"
     misc_feature    434..521
                     /label=crTRAC and crPDCD1
                     /note="crTRAC and crPDCD1"
     misc_feature    522..562
                     /label=arrayRLBC1
                     /note="arrayRLBC1"
     misc_feature    569..824
                     /label=EFS-NS
                     /note="EFS-NS"
     misc_feature    849..1565
                     /label=GFP
                     /note="GFP"
     misc_feature    1566..1613
                     /label=NLS
                     /note="NLS"
     misc_feature    1635..1682
                     /label=shortPA
                     /note="shortPA"
     gene            1711..1851
                     /label=R-ITR
     exon            1943..2249
                     /label=f1 Origin
     exon            2768..3625
                     /label=AmpR
     rep_origin      3776..4443
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttctag aagagggcct atttcccatg
      181 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
      241 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
      301 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
      361 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtaatttct
      421 actaagtgta gatgagtctc tcagctggta cactaatttc tactaagtgt agatgcacga
      481 agctctccga tgtgtaattt ctactaagtg tagatttttt taagcttggc gtggatccga
      541 tatcaactag atcttgagac aaggtaccta ggtcttgaaa ggagtgggaa ttggctccgg
      601 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      661 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      721 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      781 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggt tctagacgta
      841 cggccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      901 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      961 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1021 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1081 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1141 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1201 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1261 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1321 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1381 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1441 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1501 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1561 acaagaagcg tcctgctgct actaagaaag ctggtcaagc taagaaaaag aaataagaat
     1621 tcgatatcaa gcttaataaa agatctttat tttcattaga tctgtgtgtt ggttttttgt
     1681 gtggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     1741 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     1801 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     1861 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     1921 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1981 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     2041 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2101 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     2161 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     2221 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     2281 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     2341 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     2401 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2461 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2521 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2581 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2761 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2821 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2881 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2941 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3001 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3061 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3121 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3181 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3241 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3301 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3361 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3421 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3481 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3541 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3601 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3661 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3721 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3781 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3841 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3901 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3961 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4021 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4081 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4141 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4201 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4261 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4321 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4381 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4441 tcacatgt
//
