LOCUS       Exported                6726 bp DNA     circular SYN 16-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
COMMENT     LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     
            20-MAY-2010

            SOURCE      

              ORGANISM  

            COMMENT     VNTDATE|538328968|

            COMMENT     VNTDBDATE|538763081|

            COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|

            COMMENT     VNTAUTHORNAME|William Hendriks|

            COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|

            COMMENT     VNTAUTHORNAME|Feng Zhang|

            COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     source          1..6726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..141
                     /label=L-ITR
     misc_feature    150
                     /label=MluI
                     /note="MluI"
     misc_feature    151..611
                     /label=Brie_INS_R
                     /note="Brie_INS_R"
     primer_bind     151..183
                     /label=HKO035
                     /note="HKO035"
     misc_feature    151..166
                     /label=pHK025-F3
                     /note="pHK025-F3"
     misc_feature    164..395
                     /label=hU6 Promoter
                     /note="hU6 Promoter"
     misc_feature    331..371
                     /label=HKO157
                     /note="HKO157"
     misc_feature    336..357
                     /label=hU6P primer
                     /note="hU6P primer"
     misc_feature    354..395
                     /label=array_F
                     /note="array_F"
     misc_feature    380..395
                     /label=Readout F priming
                     /note="Readout F priming"
     misc_feature    394..395
                     /label=array_F_anneal
                     /note="array_F_anneal"
     misc_feature    396..566
                     /label=chimeric guide RNA scaffold
     misc_feature    396..566
                     /label=chimeric guide RNA scaffold
                     /note="chimeric guide RNA scaffold"
     misc_feature    396..454
                     /label=array_Rms2_anneal
                     /note="array_Rms2_anneal"
     misc_feature    396..413
                     /label=Readout_priming_site_F
                     /note="Readout_priming_site_F"
     misc_feature    396..413
                     /label=Readout_priming_site_long_F
                     /note="Readout_priming_site_long_F"
     promoter        396..412
                     /label=Human U6 Promoter
     misc_feature    396..412
                     /label=misc_feature 1
     misc_feature    413..431
                     /label=chimeric RNA backbone 1 (SapI)
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    414..420
                     /label=SapI
                     /note="SapI"
     misc_feature    423..429
                     /label=SapI
                     /note="SapI"
     misc_feature    431
                     /label=3'
                     /note="3'"
     misc_feature    443..476
                     /label=MS2 binding loop
     misc_feature    443..476
                     /label=MS2 binding loop
                     /note="MS2 binding loop"
     misc_feature    491..515
                     /label=Readout_Rms2_priming
                     /note="Readout_Rms2_priming"
     misc_feature    513..546
                     /label=MS2 binding loop
     misc_feature    513..546
                     /label=MS2 binding loop
                     /note="MS2 binding loop"
     misc_feature    540..559
                     /label=Readout_BC_R_New
                     /note="Readout_BC_R_New"
     misc_feature    567..572
                     /label=U6 terminator
     misc_feature    567..572
                     /label=U6 Terminator
                     /note="U6 Terminator"
     misc_feature    582..611
                     /label=U6(MS2)-R
                     /note="U6(MS2)-R"
     misc_feature    726..1904
                     /label=EF1a
                     /note="EF1a"
     misc_feature    750..766
                     /label=EF1a-SR1
                     /note="EF1a-SR1"
     misc_feature    1920..2306
                     /label=ms2-n55k
                     /note="ms2-n55k"
     misc_feature    2361..2381
                     /label=SV40 NLS
                     /note="SV40 NLS"
     misc_feature    2397..2939
                     /label=P65
                     /note="P65"
     misc_feature    2964..3335
                     /label=HSF1
                     /note="HSF1"
     misc_feature    3314..3339
                     /label=pGW05-R1
                     /note="pGW05-R1"
     misc_feature    3340..3381
                     /label=Fluc-R2
                     /note="Fluc-R2"
     misc_RNA        3360..3948
                     /label=WPRE
     misc_feature    3419..3444
                     /label=pHKO26-R1
                     /note="pHKO26-R1"
     gene            3989..4129
                     /label=R-ITR
     exon            4221..4527
                     /label=f1 Origin
     exon            5046..5903
                     /label=AmpR
     rep_origin      6054..6721
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttctag aaagagggcc tatttcccat
      181 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
      241 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
      301 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
      361 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggaagagc
      421 gagctcttct gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc
      481 aagttaaaat aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc
      541 agggccaagt ggcaccgagt cggtgctttt ttggatccaa gcttggcgta actagatctt
      601 gagacaaatg ggacagcaga gatccagttt ggttaattag ctagctgcaa agatggataa
      661 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
      721 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
      781 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      841 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      901 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      961 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     1021 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     1081 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     1141 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     1201 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     1261 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     1321 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     1381 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     1441 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     1501 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     1561 gaaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     1621 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     1681 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1741 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1801 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1861 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg gccaccatgg
     1921 cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat gtgacagtgg
     1981 ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca cggagccagg
     2041 cctacaaggt gacatgcagc gtcaggcagt ctagtgccca gaagagaaag tataccatca
     2101 aggtggaggt ccccaaagtg gctacccaga cagtgggcgg agtcgaactg cctgtcgccg
     2161 cttggaggtc ctacctgaac atggagctca ctatcccaat tttcgctacc aattctgact
     2221 gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga cggtaatcct atcccttccg
     2281 ccatcgccgc taactcaggt atctacagcg ctggaggagg tggaagcgga ggaggaggaa
     2341 gcggaggagg aggtagcgga cctaagaaaa agaggaaggt ggcggccgct ggatcccctt
     2401 cagggcagat cagcaaccag gccctggctc tggcccctag ctccgctcca gtgctggccc
     2461 agactatggt gccctctagt gctatggtgc ctctggccca gccacctgct ccagcccctg
     2521 tgctgacccc aggaccaccc cagtcactga gcgctccagt gcccaagtct acacaggccg
     2581 gcgaggggac tctgagtgaa gctctgctgc acctgcagtt cgacgctgat gaggacctgg
     2641 gagctctgct ggggaacagc accgatcccg gagtgttcac agatctggcc tccgtggaca
     2701 actctgagtt tcagcagctg ctgaatcagg gcgtgtccat gtctcatagt acagccgaac
     2761 caatgctgat ggagtacccc gaagccatta cccggctggt gaccggcagc cagcggcccc
     2821 ccgaccccgc tccaactccc ctgggaacca gcggcctgcc taatgggctg tccggagatg
     2881 aagacttctc aagcatcgct gatatggact ttagtgccct gctgtcacag atttcctcta
     2941 gtgggcaggg aggaggtgga agcggcttca gcgtggacac cagtgccctg ctggacctgt
     3001 tcagcccctc ggtgaccgtg cccgacatga gcctgcctga ccttgacagc agcctggcca
     3061 gtatccaaga gctcctgtct ccccaggagc cccccaggcc tcccgaggca gagaacagca
     3121 gcccggattc agggaagcag ctggtgcact acacagcgca gccgctgttc ctgctggacc
     3181 ccggctccgt ggacaccggg agcaacgacc tgccggtgct gtttgagctg ggagagggct
     3241 cctacttctc cgaaggggac ggcttcgccg aggaccccac catctccctg ctgacaggct
     3301 cggagcctcc caaagccaag gaccccactg tctcctaaga attcacgcgt taagtcgaca
     3361 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3421 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3481 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3541 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3601 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3661 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3721 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3781 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3841 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3901 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     3961 accaatgacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4021 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4081 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4141 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4201 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4261 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4321 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4381 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4621 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4681 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4741 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4801 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4861 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4921 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4981 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5041 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5101 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5161 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5221 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5281 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5341 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5401 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5461 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5521 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5581 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5641 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5701 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5761 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5821 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5881 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5941 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6001 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6061 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6721 acatgt
//
