LOCUS       WT_IFl_WT_IFr_pU        2973 bp DNA     circular SYN 24-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    U173JHD290
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2022 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
COMMENT     http://www.genscript.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    397..693
                     /label=EcoRI-BamHI digest fragment
     misc_feature    397..567
                     /label=Leader-side integration fragment
     misc_feature    438..447
                     /label=UMdistal
     misc_feature    458..486
                     /label=IHFdistal
     misc_feature    complement(497..506)
                     /label=UMproximal
     misc_feature    525..553
                     /label=IHFproximal
     misc_feature    568..595
                     /label=repeat
     misc_feature    596..693
                     /label=Spacer-side integration fragment
     misc_feature    596..627
                     /label=spacer_1
     misc_feature    628..655
                     /label=repeat
     misc_feature    656..687
                     /label=spacer_2
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccatggacc cttttttcgg acgatttctt acgcccttat aaatcagcaa
      481 gttacgagac ctcgaaaaaa gagggtttct ggcgggaaaa actcggtatt tctttttcct
      541 tcaaatggtt ataggttttc ggagctagtt cactgccgtg taggcagcta agaaaatcag
      601 ccggacgttg tagtagtcga gcgcggtgtt cactgccgtg taggcagcta agaaagccgg
      661 gtggtgactt ctggacggcg ttcgtcaagg atccatcgga tcccgggccc gtcgactgca
      721 gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      781 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      841 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      901 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      961 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1021 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1081 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1141 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1201 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1261 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1321 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1381 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1441 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1501 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1561 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1621 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1681 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1741 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1801 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1861 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1921 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1981 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2041 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2101 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2161 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2221 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2281 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2341 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2401 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2461 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2521 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2581 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2641 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2701 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2761 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2821 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2881 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2941 ataaaaatag gcgtatcacg aggccctttc gtc
//
