LOCUS       12_Opt_IFl_Opt_I        2989 bp DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    U173JHD290
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
COMMENT     http://www.genscript.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..2989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    397..709
                     /label=EcoRI+BamHI digest
     misc_feature    397..567
                     /label=Leader-side integration fragment
     misc_feature    604..709
                     /label=Spacer-side integration fragment
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccatggacc cttttttcgg acgatttaaa aaacccttat aaatcagcaa
      481 gttacgagac ctcgaaaaaa aagggtttct ggcgggaaaa actcaaaaaa cgcttcgaaa
      541 tcaaccggtt ataggttttc ggagctagtt ttagagtcat attatttaga atggtaccaa
      601 aacatcagcc ggacgttgta gtagtcgagc gcggtgtttt agagtcatat tatttagaat
      661 ggtaccaaaa cgccgggtgg tgacttctgg acggcgttcg tcaaggatcc atcggatccc
      721 gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcatggtc atagctgttt
      781 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      841 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      961 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2881 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2941 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
