LOCUS       18_Opt_IIAl_Opt_        3059 bp DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    U173JHD290
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
COMMENT     http://www.genscript.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..3059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    397..779
                     /label=EcoRI+BamHI digest
     misc_feature    397..637
                     /label=Leader-side integration fragment
     misc_feature    674..779
                     /label=Spacer-side integration fragment
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccatggatc tctttaaata atgtggatgt cctgttttta gaacaaaggg
      481 tggtccagaa cagattccaa tatattttgg acgaaaactt ttatttgagt tatgaaaaag
      541 cttaaattgt tactgattag tggttcattc taaactgaaa tctagctatg gataagtgat
      601 gcgagtacgg aactttggaa aaaaataatt ctccgaggtt ttagagtcat attatttaga
      661 atggtaccaa aacatcagcc ggacgttgta gtagtcgagc gcggtgtttt agagtcatat
      721 tatttagaat ggtaccaaaa cgccgggtgg tgacttctgg acggcgttcg tcaaggatcc
      781 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcatggtc
      841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1681 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2821 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3001 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
