LOCUS       22_Opt_Ifl_Sym2D        2976 bp DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    U173JHD290
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
COMMENT     http://www.genscript.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..2976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    397..696
                     /label=EcoRI+BamHI digest
     misc_feature    397..570
                     /label=Leader-side integration fragment
     misc_feature    599..696
                     /label=Spacer-side integration fragment
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccatggacc cttttttcgg acgatttaaa aaacccttat aaatcagcaa
      481 gttacgagac ctgatcgaaa aaaaagggtt tctggcggga aaaactcaaa aaacgcttcg
      541 aaatcaaccg gttataggtt ttcggagcta gttcactgcc gtgtaggcag ctaagaaaat
      601 cagccggacg ttgtagtagt cgagcgcggt gttcactgcc gtgtaggcag ctaagaaagc
      661 cgggtggtga cttctggacg gcgttcgtca aggatccatc ggatcccggg cccgtcgact
      721 gcagaggcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2881 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2941 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
