LOCUS       pSV40_mD6Ertd527e-HA        4275 bp    DNA     circular SYN 25-SEP-2019
DEFINITION  Expression vecotr of HA-tagged mouse D6Ertd527e gene.
FEATURES             Location/Qualifiers
     promoter        7..425
                     /vntifkey="29"
                     /label=SV40\early\enhancer/promoter
                     /note="SV40 early enhancer/promoter region"
     intron          486..622
                     /vntifkey="15"
                     /label=Synthetic\intron
                     /note="chimeric intron"
     promoter        666..683
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="T7 RNA polymerase promoter"
     polyA_signal    2221..2442
                     /vntifkey="25"
                     /label=SV40\late\polyA
                     /note="SV40 late poly(A) region"
     CDS             2589..3449
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAK53373.1"
                     /db_xref="GI:14039728"
                     /vntifkey="4"
                     /label=Amp_resistance
                     /note="ampicillin resistance"
     CDS             773..2161
                     /vntifkey="4"
                     /label=mD6Ertd527e
                     /note="mD6Ertd527e/D6Ertd527e insert"
     misc_signal     2165..2191
                     /vntifkey="87"
                     /label=HA-tag
                     /note="HA-tag"
     5'UTR           692..772
                     /vntifkey="52"
                     /label=5'-UTR
                     /note="5'-UTR (exon 1)"
BASE COUNT     1245 a      1157 c      1023 g       850 t 
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 
      661 gtacttaata cgactcacta taggctagcg gagcaagcct gtaacaagtt cctccgtggg 
      721 ctctgcattg gttcctgcct ctaggaacct cctgccctga cttctctgga tgatggacta 
      781 caagttcagt agccgccgcc gctgcagcag cagcagtcgg agcagcctca gcagccgcag 
      841 cagtgacacc agcaccagca gtgacaccag cagtgacacc agcaccagca ctagcaccag 
      901 caccagcacc agccacagca acaacagcag cagcaacagc agcagaaaac ctagtaacaa 
      961 aggcagcagc agcctctcga gcagcagcag caacagcagc agcaaaccta gtgacacaga 
     1021 cagcaacagc agcagcatct cttgcagcag caacagccct agtaacacag acagcagcag 
     1081 cctctctagc agcagcagca acagcagcag acctagtaac acaggcagca gcagcctctc 
     1141 tagcagcagc agcaacagca gcagacctag taacacaggc agcagtagca gcagcagcag 
     1201 caacagcagc aacatcagca acagcagcat cagacctagt aacaggggca gcatcagcaa 
     1261 ctatgacaac agcagcaaca gcagcagtcc ccaaccctct agtggcaaca tcagcaacag 
     1321 aagacctagt aacacaggca gcagcagcaa ccaggttaac agcggcccca gacctggtaa 
     1381 cacagtcaac atcagcaact atagcaacag tggccccaga cccagtaaca ccaccaccag 
     1441 cagcaacagc caaagcaaca gcagccccag acctagtaac aggggcagca tcagcaacta 
     1501 cagcaacagc agcctcagac ctagtaacag gggcaacatc agcaactatg acaacagcag 
     1561 caccagacct agtaacaggg ccaacatcag caactatggc aacagcagca atagcagcag 
     1621 tccccaaccc tctagcagca aaatcagcaa cagaagacct agtaacacag gcagcagcag 
     1681 caaccaggtt aacagcggcc ccagacctgg aaacacagtc aacatcagca actatagcaa 
     1741 cagtggcccc agacccagta acaccaccag cagcagcaac agccaaagca acagcagccc 
     1801 cagatctagt aacacagaca gcagcagcag ccctggcaac agccacacca gtagcagcag 
     1861 cagcagcagc agcagcagca gcagcagcag tagcagcagc cgcggcaaca gtggccccag 
     1921 acccagtaaa acaggcagca taagcagcca aagcaacagc ggccccagat ctagtaacac 
     1981 agacagcagc agcagcagca gccccggcaa tatcaaaacc agcagcagca gcagcagcag 
     2041 taacagcagc agcagcagcc acagcagcta cagctgcagc agcagcagcc acagcagcag 
     2101 cagcagccgc agccatagca gcagccacag tcacagcagc cacagtcgca caccatggga 
     2161 agcctaccca tacgacgtcc cagactacgc ttctagatga ggttctgcgg ccgcttcgag 
     2221 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 
     2281 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 
     2341 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 
     2401 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccag 
     2461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     2521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     2581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     2641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     2701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     2761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     2821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     2881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     2941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     3001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     3061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     3121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     3181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     3241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     3301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     3361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     3421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     3481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     3541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     3601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     3661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     3721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     3781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     3841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     3901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     3961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     4021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     4081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     4141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     4201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     4261 ctcacatggc tcgac 
//
