LOCUS       pSV40_mD6Ertd527e-HA-EGFP        4991 bp    DNA     circular SYN 25-SEP-2019
DEFINITION  Expression vecotr of HA-EGFP-tagged mouse D6Ertd527e gene.
FEATURES             Location/Qualifiers
     promoter        7..425
                     /vntifkey="29"
                     /label=SV40\early\enhancer/promoter
                     /note="SV40 early enhancer/promoter region"
     intron          486..622
                     /vntifkey="15"
                     /label=Synthetic\intron
                     /note="chimeric intron"
     promoter        666..683
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="T7 RNA polymerase promoter"
     polyA_signal    2937..3158
                     /vntifkey="25"
                     /label=SV40\late\polyA
                     /note="SV40 late poly(A) region"
     CDS             3305..4165
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAK53373.1"
                     /db_xref="GI:14039728"
                     /vntifkey="4"
                     /label=Amp_resistance
                     /note="ampicillin resistance"
     CDS             773..2161
                     /vntifkey="4"
                     /label=mD6Ertd527e
                     /note="mD6Ertd527e/mD6Ertd527e insert"
     misc_signal     2165..2191
                     /vntifkey="87"
                     /label=HA-tag
                     /note="HA-tag"
     5'UTR           692..772
                     /vntifkey="52"
                     /label=5'-UTR
                     /note="5'-UTR (exon 1)"
     CDS             2198..2923
                     /vntifkey="4"
                     /label=EGFP
                     /note="EGFP"
BASE COUNT     1418 a      1400 c      1223 g       950 t 
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 
      661 gtacttaata cgactcacta taggctagcg gagcaagcct gtaacaagtt cctccgtggg 
      721 ctctgcattg gttcctgcct ctaggaacct cctgccctga cttctctgga tgatggacta 
      781 caagttcagt agccgccgcc gctgcagcag cagcagtcgg agcagcctca gcagccgcag 
      841 cagtgacacc agcaccagca gtgacaccag cagtgacacc agcaccagca ctagcaccag 
      901 caccagcacc agccacagca acaacagcag cagcaacagc agcagaaaac ctagtaacaa 
      961 aggcagcagc agcctctcga gcagcagcag caacagcagc agcaaaccta gtgacacaga 
     1021 cagcaacagc agcagcatct cttgcagcag caacagccct agtaacacag acagcagcag 
     1081 cctctctagc agcagcagca acagcagcag acctagtaac acaggcagca gcagcctctc 
     1141 tagcagcagc agcaacagca gcagacctag taacacaggc agcagtagca gcagcagcag 
     1201 caacagcagc aacatcagca acagcagcat cagacctagt aacaggggca gcatcagcaa 
     1261 ctatgacaac agcagcaaca gcagcagtcc ccaaccctct agtggcaaca tcagcaacag 
     1321 aagacctagt aacacaggca gcagcagcaa ccaggttaac agcggcccca gacctggtaa 
     1381 cacagtcaac atcagcaact atagcaacag tggccccaga cccagtaaca ccaccaccag 
     1441 cagcaacagc caaagcaaca gcagccccag acctagtaac aggggcagca tcagcaacta 
     1501 cagcaacagc agcctcagac ctagtaacag gggcaacatc agcaactatg acaacagcag 
     1561 caccagacct agtaacaggg ccaacatcag caactatggc aacagcagca atagcagcag 
     1621 tccccaaccc tctagcagca aaatcagcaa cagaagacct agtaacacag gcagcagcag 
     1681 caaccaggtt aacagcggcc ccagacctgg aaacacagtc aacatcagca actatagcaa 
     1741 cagtggcccc agacccagta acaccaccag cagcagcaac agccaaagca acagcagccc 
     1801 cagatctagt aacacagaca gcagcagcag ccctggcaac agccacacca gtagcagcag 
     1861 cagcagcagc agcagcagca gcagcagcag tagcagcagc cgcggcaaca gtggccccag 
     1921 acccagtaaa acaggcagca taagcagcca aagcaacagc ggccccagat ctagtaacac 
     1981 agacagcagc agcagcagca gccccggcaa tatcaaaacc agcagcagca gcagcagcag 
     2041 taacagcagc agcagcagcc acagcagcta cagctgcagc agcagcagcc acagcagcag 
     2101 cagcagccgc agccatagca gcagccacag tcacagcagc cacagtcgca caccatggga 
     2161 agcctaccca tacgacgtcc cagactacgc ttctagagtg agcaagggcg aggagctgtt 
     2221 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 
     2281 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 
     2341 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 
     2401 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 
     2461 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 
     2521 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 
     2581 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 
     2641 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 
     2701 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 
     2761 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 
     2821 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 
     2881 gatcactctc ggcatggacg agctgtacaa gtccggactc taacggccgc ttcgagcaga 
     2941 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 
     3001 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 
     3061 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 
     3121 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg 
     3181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 
     3241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 
     3301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 
     3361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 
     3421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 
     3481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 
     3541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 
     3601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 
     3661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 
     3721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 
     3781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 
     3841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 
     3901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 
     3961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 
     4021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 
     4081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 
     4141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 
     4201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 
     4261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 
     4321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 
     4381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 
     4441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 
     4501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 
     4561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 
     4621 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 
     4681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 
     4741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 
     4801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 
     4861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 
     4921 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 
     4981 catggctcga c 
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