LOCUS       pCL219                 7711 bp    DNA     circular SYN 12-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(4217..5011)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(6245..6952)
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      5536..5964
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(5046..5403)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(2543..2846)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(2339..2542)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(6977..7711,1..2307))
                     /note="pLannotate"
                     /label="PARP1"
                     /database="swissprot"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(5998..6119)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(5405..5509)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(3021..3609)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3938..3985)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(4044..4196)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6142..6225)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cagcaggttg tcaagcattt ccaccttggc ctgcacactg tctgcattgt tcaggagcgg
       61 aggcttcttc atcccaaagt cgtgggggat cagggtgtaa aagcgatttg agagatccag
      121 gatctgagag tcgctgctgc cctgagacac cgcctgctgg acctcactga ggatggagta
      181 tgcggcctgg atctgccttt tgctcagctt ccccaagggc atcttctgaa ggtcgatctc
      241 atactccacc atggctttct tcatactttc cacatcaaag atcatcttga tgaggtcctg
      301 aactggcttg gggagcttgg acttggtgcc aggatttact gtcagcttct tcactgcctc
      361 ttcatcctgg ccatagtcaa tctccagggg gtagaacttt ttgggatact tcgtgaaatt
      421 tttggagtgc caagcgttcc cggttttttc ttcatataat ttcatgaagt gctcaatggc
      481 atcctccttg gacggcatct gttccagttt gttgctaccg atcaccgtac ccacacggcc
      541 ccaggacctg aatatccaat acctgttttc cttgtcgtcc tccagaagct ggagcttgta
      601 gtaggagttg gttcctttaa cgatgtccac caggccaagg gtggcactga agaccttccc
      661 acctttctcc aggacatgcg cagagtgttc cagtccagaa tcaggatcca cagctgctcc
      721 tcctttaaga gttaatttca ttctcttttc agatttgttg ataccttcct ccttgacctg
      781 gcccttgctt tttttggaga gcgcagcccc tgacttccct cttggggcca caacttcaac
      841 aggctctgcc ttcacctctg ccccccaagg ggacaagatg tgcgctaaga acaactcctg
      901 aaggctcttg gtggaggcgg agacgtcctg gaggaagtcc tcagacacaa ctcggatgtt
      961 ggcttccttt acttcctcca tcttcttatt catcttttcc acctcctttt tggtgctgat
     1021 gcacagggaa gccttgttgg ccgtccccgt caacttcccc ccgagtttct caatcatggc
     1081 cttcacttca tccttgttcc gggacagctt cccgagagtc aggatcttca tgttggataa
     1141 tggcttatct gctgaagcag aggagttcac agcagcagga gccgaggctg tggagggcgg
     1201 aggcgtggcc gccacggagg cgctggtttc tggggggaat atacggtcct gctttttaac
     1261 cttcaatttc ttgaggtaag agatttctcg gaattccttt ggggttaccc actccttccg
     1321 gttgggtgtc tgtgtcttga ccatacactt ggtccaggca gtgacgtccc cagtgcagta
     1381 ataggcatcg ctcttgaaga ccagctgacc cgagcattcc tcgcagggaa ggagggcacc
     1441 gaacaccatg ccatcagcta ctcggtccaa gatcgccgac tccccagaag gcacttgctg
     1501 cttgttgaag atgagtagct ccttcaggtc attagttgaa cacactttct ttagctcgtc
     1561 cttgatgttc cagatcaggt cgttctgagc ctttagggct ttttcaagct tactatcctt
     1621 gtctttttct tttttagatt tcttcttcgc cacttcatcc actccatcca cctcatcgcc
     1681 ttttctcttt ccttcactct tgactcctgg gagctgcttc ttcagggctt ccttatcctc
     1741 tgtagcaagg aggctgaagc ccttgagctg actcgcactg tactcgggcc ggaaacccag
     1801 ctcctccctg ttcttgacaa agcagcctgg atggtaccag cggtcaatca tgcctagctg
     1861 tggcttctcc gggtccacca tcttcttgga caggcgcacc tggccctttt ctatcttctc
     1921 catacacccc ttgcacgtac ttctgttgga cttggcatac tctgctgcaa agtcacccag
     1981 agtcttctct gccttgctac caattccatc ctggcctttg cctgtcactc ctccagcttc
     2041 cgctgtcttc ttgactttct gctggtcatc ccaccgaagc tcagagaacc catccacctc
     2101 aacgtcaggg tgccggatgg agtggcccac cttccagaag caggagaagt ggtaccagtg
     2161 tgggactttt ccatcaaaca tgggcgactg caccatgatg gccatccgga gcgagtcctt
     2221 ggggatgctc tcgctgcatt tcttgcaaga ggcgcgcccg ctcttggcgt actcgactcg
     2281 atagagctta tccgaagact ccgccatggt gctagcggat ctgacggttc actaaaccag
     2341 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     2401 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     2461 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     2521 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     2581 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     2641 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     2701 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     2761 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     2821 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     2881 ccgtaattga ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca
     2941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
     3781 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
     3841 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
     3901 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
     3961 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
     4021 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
     4081 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     4141 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     4201 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     4261 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     4321 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     4381 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4441 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     4501 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     4561 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     4621 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     4681 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     4741 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     4801 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     4861 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4921 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4981 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     5041 cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     5101 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     5161 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     5221 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     5281 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     5341 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     5401 caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5521 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5581 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5641 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5701 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5761 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5821 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5881 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5941 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tacgccttaa
     6001 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6061 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     6121 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     6181 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
     6241 tttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact
     6301 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt
     6361 aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt
     6421 agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct
     6481 cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc
     6541 cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca
     6601 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
     6661 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
     6721 cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg
     6781 ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
     6841 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
     6901 gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc atggtggcga
     6961 ccggtggatc ccgggaccac agggaggtct taaaattgaa tttcagtttc agcagatact
     7021 tcagatttac ctgagcaata tcatagacaa tgtactcgtt atatagtaga gaggtgtcat
     7081 tcacaccaga tgaaatcccg gtcccaagag gaacgtctac accatccaga ctaatgttag
     7141 ctgaaggatc aggggtagtt ttgcccaaac ctttgacact gtgcttgccc ttgggtaact
     7201 tgctgatatg tgaagcgtgc ttcagttcat acatgtttcc aagggcaact tctcccaaca
     7261 ggattaagcc tattgggtct ccctgagacg tatggcagta gttggcactc ttggagacca
     7321 tgtcagcgaa atagatccct ttaccaaaca tgtagcctgt cacgggcgct tcaggcgggg
     7381 ctatccgaag accctgggac aggatcccag caaagttggt ggtcctggac ccgtgccaca
     7441 gcaatcttcg gttatgaagc tgcttaaagg gcttgtaacg ctggcattcg ccttcacgct
     7501 ctatcttaaa gatatcgatg acttccaagt catacgcact gtgtgtggtt gcatgagtgt
     7561 tcttaacata cttcctgatg atctcggctt cttcagaatc tctgtcaacc accttaatgt
     7621 cagttttgag cttctcatag ttgacatcga tgggatcctt gctgctatca tcagaccctc
     7681 ccctgagcag actgtaggcc acctcgatgg c
//
