LOCUS       pCL220                 7711 bp    DNA     circular SYN 12-MAY-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2533..3327
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             592..1299
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1580..2008)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        2141..2498
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        4698..5001
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        5002..5205
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(5237..7711,1..567)
                     /note="pLannotate"
                     /label="PARP1"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    1425..1546
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        2035..2139
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      3935..4523
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    3559..3606
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             3348..3500
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             1319..1402
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cacaatgcgt atgacttgga agtcatcgat atctttaaga tagagcgtga aggcgaatgc
       61 cagcgttaca agccctttaa gcagcttcat aaccgaagat tgctgtggca cgggtccagg
      121 accaccaact ttgctgggat cctgtcccag ggtcttgcca tagccccgcc tgaagcgccc
      181 gtgacaggct acatgtttgg taaagggatc tatttcgctg acatggtctc caagagtgcc
      241 aactactacc atacgtctca gggagaccca ataggcttaa tcctgttggg agaagttgcc
      301 cttggaaaca tgtatgaact gaagcacgct tcacatatca gcaggttacc caagggcaag
      361 cacagtgtca aaggtttggg caaaactacc cctgatcctt cagctaacat tagtctggat
      421 ggtgtagacg ttcctcttgg gaccgggatt tcatctggtg tgatagacac ctctctacta
      481 tataacgagt acattgtcta tgatattgct caggtaaatc tgaagtatct gctgaaactg
      541 aaattcaatt ttaagacctc cctgtggtcc cgggatccac cggtcgccac catggtgagc
      601 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      661 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      721 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      781 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      841 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      901 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      961 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     1021 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     1081 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     1141 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     1201 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     1261 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1321 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     1381 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     1441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
     1561 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     1621 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     1681 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     1741 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     1801 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     1861 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     1921 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     1981 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2041 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2101 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     2161 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2221 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2341 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2401 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2461 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     2521 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     2581 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     2641 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     2701 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     2761 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     2821 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     2881 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     2941 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3001 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     3061 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     3121 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     3181 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     3241 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     3301 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     3361 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     3421 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     3481 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     3541 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     3601 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     3661 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     3721 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     3781 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3841 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3901 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3961 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4021 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4081 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4141 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4201 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4261 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4321 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4381 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4441 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4501 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4561 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4621 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     4681 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4741 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4801 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4861 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4921 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4981 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     5041 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5101 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     5161 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     5221 cagatccgct agcaccatgg cggagtcttc ggataagctc tatcgagtcg agtacgccaa
     5281 gagcgggcgc gcctcttgca agaaatgcag cgagagcatc cccaaggact cgctccggat
     5341 ggccatcatg gtgcagtcgc ccatgtttga tggaaaagtc ccacactggt accacttctc
     5401 ctgcttctgg aaggtgggcc actccatccg gcaccctgac gttgaggtgg atgggttctc
     5461 tgagcttcgg tgggatgacc agcagaaagt caagaagaca gcggaagctg gaggagtgac
     5521 aggcaaaggc caggatggaa ttggtagcaa ggcagagaag actctgggtg actttgcagc
     5581 agagtatgcc aagtccaaca gaagtacgtg caaggggtgt atggagaaga tagaaaaggg
     5641 ccaggtgcgc ctgtccaaga agatggtgga cccggagaag ccacagctag gcatgattga
     5701 ccgctggtac catccaggct gctttgtcaa gaacagggag gagctgggtt tccggcccga
     5761 gtacagtgcg agtcagctca agggcttcag cctccttgct acagaggata aggaagccct
     5821 gaagaagcag ctcccaggag tcaagagtga aggaaagaga aaaggcgatg aggtggatgg
     5881 agtggatgaa gtggcgaaga agaaatctaa aaaagaaaaa gacaaggata gtaagcttga
     5941 aaaagcccta aaggctcaga acgacctgat ctggaacatc aaggacgagc taaagaaagt
     6001 gtgttcaact aatgacctga aggagctact catcttcaac aagcagcaag tgccttctgg
     6061 ggagtcggcg atcttggacc gagtagctga tggcatggtg ttcggtgccc tccttccctg
     6121 cgaggaatgc tcgggtcagc tggtcttcaa gagcgatgcc tattactgca ctggggacgt
     6181 cactgcctgg accaagtgta tggtcaagac acagacaccc aaccggaagg agtgggtaac
     6241 cccaaaggaa ttccgagaaa tctcttacct caagaaattg aaggttaaaa agcaggaccg
     6301 tatattcccc ccagaaacca gcgcctccgt ggcggccacg cctccgccct ccacagcctc
     6361 ggctcctgct gctgtgaact cctctgcttc agcagataag ccattatcca acatgaagat
     6421 cctgactctc gggaagctgt cccggaacaa ggatgaagtg aaggccatga ttgagaaact
     6481 cggggggaag ttgacgggga cggccaacaa ggcttccctg tgcatcagca ccaaaaagga
     6541 ggtggaaaag atgaataaga agatggagga agtaaaggaa gccaacatcc gagttgtgtc
     6601 tgaggacttc ctccaggacg tctccgcctc caccaagagc cttcaggagt tgttcttagc
     6661 gcacatcttg tccccttggg gggcagaggt gaaggcagag cctgttgaag ttgtggcccc
     6721 aagagggaag tcaggggctg cgctctccaa aaaaagcaag ggccaggtca aggaggaagg
     6781 tatcaacaaa tctgaaaaga gaatgaaatt aactcttaaa ggaggagcag ctgtggatcc
     6841 tgattctgga ctggaacact ctgcgcatgt cctggagaaa ggtgggaagg tcttcagtgc
     6901 cacccttggc ctggtggaca tcgttaaagg aaccaactcc tactacaagc tccagcttct
     6961 ggaggacgac aaggaaaaca ggtattggat attcaggtcc tggggccgtg tgggtacggt
     7021 gatcggtagc aacaaactgg aacagatgcc gtccaaggag gatgccattg agcacttcat
     7081 gaaattatat gaagaaaaaa ccgggaacgc ttggcactcc aaaaatttca cgaagtatcc
     7141 caaaaagttc taccccctgg agattgacta tggccaggat gaagaggcag tgaagaagct
     7201 gacagtaaat cctggcacca agtccaagct ccccaagcca gttcaggacc tcatcaagat
     7261 gatctttgat gtggaaagta tgaagaaagc catggtggag tatgagatcg accttcagaa
     7321 gatgcccttg gggaagctga gcaaaaggca gatccaggcc gcatactcca tcctcagtga
     7381 ggtccagcag gcggtgtctc agggcagcag cgactctcag atcctggatc tctcaaatcg
     7441 cttttacacc ctgatccccc acgactttgg gatgaagaag cctccgctcc tgaacaatgc
     7501 agacagtgtg caggccaagg cggaaatgct tgacaacctg ctggacatcg aggtggccta
     7561 cagtctgctc aggggagggt ctgatgatag cagcaaggat cccatcgatg tcaactatga
     7621 gaagctcaaa actgacatta aggtggttga cagagattct gaagaagccg agatcatcag
     7681 gaagtatgtt aagaacactc atgcaaccac a
//
