LOCUS       iCAP-ROOT               9378 bp DNA     circular SYN 19-SEP-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    iCAP-ROOT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
            Sequenced at PlasmidSaurus 03/05/2022
COMMENT     Alias: iCAP-ROOT
FEATURES             Location/Qualifiers
     source          1..9378
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    886..1553
                     /label=pUC ori
                     /note="color: #a6acb3"
     rep_origin      909..1497
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     misc_feature    1680..1894
                     /label=LacZ promoter
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1680..1772
                     /codon_start=1
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1785..1806
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        1821..1851
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    1859..1875
                     /label=rlac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     primer_bind     1883..1899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        1920..1938
                     /label=rT3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     misc_feature    1951..1979
                     /label=rMCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=26.9"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     misc_feature    2023..2153
                     /label=p5 promoter
                     /note="color: #a6acb3"
     CDS             complement(2246..4474)
                     /codon_start=1
                     /label=rVP1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=81.0"
                     /note="/match_length=101.1"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGL
                     VLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERL
                     KEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSG
                     AQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVG
                     SSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPW
                     GYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTS
                     TVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEY
                     FPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTINGSGQNQ
                     QTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSL
                     MNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATES
                     YGQVATNHQSAQIPNMGDHAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGN
                     FHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWEL
                     QKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL"
     misc_feature    2690..2710
                     /label=ROOT insert
                     /note="color: #993366"
     CDS             complement(4494..4794)
                     /label=AAV 2 Rep
                     /note="color: #993366; direction: LEFT"
     misc_feature    4795..5043
                     /label=p41 promoter
                     /note="color: #a6acb3"
     promoter        complement(5117..5137)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5118..5136
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5118..5136
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5118..5136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5154..5174)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5155..5173
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5155..5173
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5155..5173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5191..5211)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5192..5210
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5192..5210
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5192..5210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5228..5248)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5229..5247
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5229..5247
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5229..5247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5265..5285)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5266..5284
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5266..5284
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5266..5284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5302..5322)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5303..5321
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5303..5321
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5303..5321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5339..5359)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5340..5358
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5340..5358
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5340..5358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5376..5396)
                     /label=rtight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    5377..5395
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5377..5395
                     /label=Tet binding
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    5377..5395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     promoter        complement(5432..5453)
                     /label=rSV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    complement(5457..5681)
                     /label=rbGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        5707..5725
                     /label=rSP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             complement(5755..6501)
                     /codon_start=1
                     /label=tTA
                     /note="color: #993366"
                     /translation="MSRLDKSKVINGALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(5764..5874)
                     /codon_start=1
                     /label=rVP48
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(5804..6480)
                     /codon_start=1
                     /label=rrtTA3 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.7"
                     /note="/match_length=95.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="KVINGALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALL
                     DALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYE
                     TLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLL
                     RQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPADALDDFD
                     LDM"
     CDS             complement(6521..8383)
                     /codon_start=1
                     /label=rRep78
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERLNQNSNICFTHGVKDCLECFPVSE
                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCVSEQ"
     CDS             complement(6521..6821)
                     /label=AAV 2 Rep
                     /note="color: #993366; direction: LEFT"
     promoter        complement(8484..8502)
                     /label=rT7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     CDS             8510..8665
                     /codon_start=1
                     /label=rlacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     rep_origin      8670..9098
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        9151..9255
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(9256..9378,1..738)
                     /codon_start=1
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1681 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1741 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1801 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1861 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     1921 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctagt
     1981 cgagcggccg ccagtgtgat ggatatctgc agaattcggc ttggcggctg cgcgttcaaa
     2041 cctcccgctt caaaatggag accctgcgtg ctcactcggg cttaaatacc cagcgtgacc
     2101 acatggtgtc gcaaaatgtc gcaaaacact cacgtgacct ctaatacagg acctctagtc
     2161 ctgcaggttt aaacgaattc gcccttcgca gagaccaaag ttcaactgaa acgaattaaa
     2221 cggtttattg attaacaagc aattacagat tacgagtcag gtatctggtg ccaatggggc
     2281 ggggttcact atatacacct tcagtattaa cagcaaattc aacattatta gacttgtaat
     2341 agttggaagt gtactggatc tccgggttcc agcgcttgct gttttccttc tgcagctccc
     2401 actcgatctc cacgctgact tggccagtag aatactgggt gatgaaagag ttcagcttgt
     2461 ccttgttgaa ggccgttgga ggatccgcag gtacaggtgt gtttttgatg aggatctgag
     2521 gaggcgggtg cttcattcca aaccctccca tcagcggaga agggtgaaag ttgccgtccg
     2581 tgtgaggaat tttggcccaa atgggtcctt gcaggtacac atctctgtcc tgccaaacca
     2641 tacccggaag tattccttgg ttttgaaccc aaccggtctg cgcctgtgca tgatccccca
     2701 tattaggaat ttgggcactc tggtggtttg tggccacttg tccataggac tccgttgcta
     2761 ccgggttagt agttttaatt tcttcttcgt tggttatcat gactttgtcc gcatccacgt
     2821 tgtctcttcc agttccttgt ttgccaaaaa ttaaagatcc agacaaagga aagaaacggt
     2881 cctctccttc tttgtggctg gccatagcag gtccaggatt catcaagcta ttacgtccat
     2941 tgagagccca agaagaagct ccaggccaag caaattcgct gttgttgttt tgagtcacag
     3001 tggttgagac acgttgttgt cggtagctgg gtccaggtat gtagtttctt ccctggacag
     3061 ccatgttgct gggtccggcc acactgaatt ttagcgtttg ttgattctgt ccagaaccgt
     3121 taatagttct agagagatag tacaagtatt ggtcgatgag tggattcatt agtcggtcca
     3181 ggctttggct gtgagcgtag ctgctatgga aaggtacgtt ctcaaactcg tagctgaact
     3241 ggaagttgtt acccgttctt agcatttgcg acgggaaata ttccaggcag taaaaggacg
     3301 aacgacccac ggcctggctt ccatcattaa gcgtcagata cccgtactga ggaatcatga
     3361 aaacgtccgc tgggaacggc gggaggcagc cctcgtgagc cgacccgagc acgtacggga
     3421 gctgatagtc tgagtccgtg aagacctgga ccgtgctggt aaggttattg gcgatggtct
     3481 tgactccatt gttgtccgta acctctttga cctgaatgtt gaagagcttg aagttgagtc
     3541 gcttaggccg gaatccccag ttgttgttga tgagtcgctg ccagtcacgt ggtgagaagt
     3601 ggcagtggaa tctgttgaag tcaaaatacc cccagggggt gctgtagccg aagtaggcgt
     3661 tgtcatttga agatcctcca gatgtgctgt tggagatttg cttgtagagg tgattgttgt
     3721 aggtgggcag ggcccaggtt cgggtgctgg tggtgatgac tctgtccccc agccattggg
     3781 aatcgcaatg ccaatttccc gaggaactac ccactccatc ggcaccttcg ttattgtctg
     3841 ccactggtgc gccaccacct gaagccattg taagagatcc cacacctgag ggggctgcgg
     3901 gaggttctcc gattggttga gggtctggga ctgactctgt gtcgccagtc tgaccgaaat
     3961 tgagtctctt tttagcgggc tgtgcacccg atttgccaat acccgcggag gagtccggtt
     4021 cctgaggaga ctgctctaca ggcctcttct ttccaggagc cgtcttagcc gcttcctcaa
     4081 ccagaccaag aggttcaaga agcctctttt tggcctggaa gactgctcgc ccgaggttgc
     4141 ccccaaaaga cgtatcttct ttgagccgct cctggaactc ggcgtcggcg tggttgtact
     4201 tgaggtacgg gttgtctccg gccttgagct gctggtcgta ggccttgtcg tgctcgaggg
     4261 ccgccgcgtc tgctgcgttg accggctccc ccttgtcgag tccgttgccg ggtccaaggt
     4321 atttgtaacc cggaagcaca agacctcgag cgttgtcttg atgttgttga tttgccttgg
     4381 gttgaggggc tccaggtttc aaagcccacc actcgcgaat tccttcacta aggttgtcct
     4441 cgagccaatc tggaagataa ccatcggcag ccatacctgg tttaagtcat ttattgttca
     4501 gaaacacagt catccaagtc cacattgacc aggtcgcaag cagtgcaagc gtctggcacc
     4561 tttcccatga tgtgatgaat gtagcacagt ttctgatacg cctttttgac gacagaaacg
     4621 ggttgagatt ctgacacggg aaagcactct aaacagtctt tgacaccgtg agtgaagcag
     4681 atatttgaat tctgattcag tctctcgcat tgtctgcagg gaaacagcat cagattcatg
     4741 cccacgtgac gagaacattt gttttggtac ctgtccgcgt agttgatcga agctagcggt
     4801 cgcagaggag cgggatcaac agtcacgtct gaactgcgag gcgtctcggg aacaaatgag
     4861 agcctggccc gcttctccag acttttatag ctagtattgg tgacgtcacc cagtgggcgt
     4921 tttagagatt tctccgcccc tttagttccc gccaattccc tgggaacttt aaactcgtga
     4981 gtcaccggca cctgattgac ctttgcccaa gcaaaaaagt ccttgacttc ctgcttagta
     5041 atcttgccaa aatctggcgg gagccgctta gtcagttcaa atttgaacag atctctctgc
     5101 ttatataggg ggtaccttct ctatcactga tagggagctc gagacgtgtc gacttctcta
     5161 tcactgatag ggagacgttc tagacgtacc ttctctatca ctgataggga gctcgagacg
     5221 tgtcgacttc tctatcactg atagggagac gttctagacg taccttctct atcactgata
     5281 gggagctcga gacgtgtcga cttctctatc actgataggg agacgttcta gacgtacctt
     5341 ctctatcact gatagggagc tcgagacgtg tcgacttctc tatcactgat agggagacgt
     5401 caggtggcac ttgatagctt gattcgagcc ccagctggtt ctttccgcct cagaagccat
     5461 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     5521 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     5581 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     5641 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgagc
     5701 tctagcattt aggtgacact atagaatagg gccctctaga tcgaattcat gcatttaccc
     5761 ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat caaggtcaaa
     5821 gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg cgtcggccgg
     5881 cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt ccaggccgaa
     5941 aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc tcagaagtgg
     6001 gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat gctcctgttc
     6061 ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca gtgcgttctc
     6121 cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt cgtactgttt
     6181 ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga ggagagcaca
     6241 gcggaatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt ccagggggca
     6301 gaagtgggta tgatgcctgt ccagcatctc gatggccagg gcatcgagca gggcccgctt
     6361 gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag cgagtttcct
     6421 tgtcgtcagg ccttcgatac cgacttcatt gagtaattcc agagcgccgt ttatgacttt
     6481 agatttatcc agccgactca tacctggttt aagtcattta ttgttcagaa acacagtcat
     6541 ccaagtccac attgaccagg tcgcaagcag tgcaagcgtc tggcaccttt cccatgatgt
     6601 gatgaatgta gcacagtttc tgatacgcct ttttgacgac agaaacgggt tgagattctg
     6661 acacgggaaa gcactctaaa cagtctttga caccgtgagt gaagcagata tttgaattct
     6721 gattcagtct ctcgcattgt ctgcagggaa acagcatcag attcatgccc acgtgacgag
     6781 aacatttgtt ttggtacctg tccgcgtagt tgatcgaagc ttccgcgtct gacgtcgatg
     6841 gctgcgcaac tgactcgcgc acccgtttgg gctcacttat atctgcgtca ctgggggcgg
     6901 gtcttttctt ggctccaccc tttttgacgt agaattcatg ctccacctca accacgtgat
     6961 cctttgccca ccggaaaaag tctttgactt cctgcttggt gaccttccca aagtcatgat
     7021 ccagacggcg ggtgagttca aatttgaaca tccggtcttg caacggctgc tggtgttcga
     7081 aggtcgttga gttcccgtca atcacggcgc acatgttggt gttggaggtg acgatcacgg
     7141 gagtcgggtc tatctgggcc gaggacttgc atttctggtc cacgcgcacc ttgcttcctc
     7201 cgagaatggc tttggccgac tccacgacct tggcggtcat cttcccctcc tcccaccaga
     7261 tcaccatctt gtcgacacag tcgttgaagg gaaagttctc attggtccag tttacgcacc
     7321 cgtagaaggg cacagtgtgg gctatggcct ccgcgatgtt ggtcttcccg gtagttgcag
     7381 gcccaaacag ccagatggtg ttcctcttgc cgaacttttt cgtggcccat cccagaaaga
     7441 cggaagccgc atattgggga tcgtacccgt ttagttccaa aattttataa atccgattgc
     7501 tggaaatgtc ctccacgggc tgctggccca ccaggtagtc gggggcggtt ttagtcaggc
     7561 tcataatctt tcccgcattg tccaaggcag ccttgatttg ggaccgcgag ttggaggccg
     7621 cattgaagga gatgtatgag gcctggtcct cctggatcca ctgcttctcc gaggtaatcc
     7681 ccttgtccac gagccacccg accagctcca tgtacctggc tgaagttttt gatctgatca
     7741 ccggcgcatc agaattggga ttctgattct ctttgttctg ctcctgcgtc tgcgacacgt
     7801 gcgtcagatg ctgcgccacc aaccgtttac gctccgtgag attcaaacag gcgcttaaat
     7861 actgttccat attagtccac gcccactgga gctcaggctg ggttttgggg agcaagtaat
     7921 tggggatgta gcactcatcc accaccttgt tcccgcctcc ggcgccattt ctggtctttg
     7981 tgaccgcgaa ccagtttggc aaagtcggct cgatcccgcg gtaaattctc tgaatcagtt
     8041 tttcgcgaat ctgactcagg aaacgtccca aaaccatgga tttcaccccg gtggtttcca
     8101 cgagcacgtg catgtggaag tagctctctc ccttctcaaa ttgcacaaag aaaagagcct
     8161 ccggggcctt actcacacgg cgccattccg tcagaaagtc gcgctgcagc ttctcggcca
     8221 cggtcagggg tgcctgctca atcagattca gatccatgtc agaatctggc ggcaactccc
     8281 attccttctc ggccacccag ttcacaaagc tgtcagaaat gccgggcaga tgctcgtcaa
     8341 ggtcgctggg gaccttaatc acaatctcgt aaaaccccgg catggcggct gcgcgttcaa
     8401 acctcccgct tcaaaatgga gaccctgcga gctcccccga taccgtcgac ctcgatcgag
     8461 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8521 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8581 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8641 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8701 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8761 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8821 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8881 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8941 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9001 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9061 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9121 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9181 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9241 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9301 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9361 gatcagttgg gtgcacga
//
