LOCUS       Copy_of_pCas-Cas12m-ΔZF 5849 bp ds-DNA     circular     20-SEP-2022
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_1848"
FEATURES             Location/Qualifiers
     primer          382..401
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    496..530
                     /label="PJ23108"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    529..547
                     /label="FlankL"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    548..2338
                     /label="VU1_Mmu_nuclease"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             548..2338
                     /label="Translation 548-2338"
     misc_feature    2192..2194
                     /label="H549A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2201..2203
                     /label="C552A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2339..2357
                     /label="FlankR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2377..2394)
                     /label="pTrcHis_rev_primer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2377..2394)
                     /label="pBAD_rev_primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      2427..2584
                     /label="rrnB_terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2550..2593
                     /label="rrnB_T1_terminator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     terminator      2725..2752
                     /label="rrnB_T2_terminator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        2793..2821
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            2990..3274
                     /label="Ampicillin (576 - 860)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     origin          3333..3632
                     /label="f1_origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            complement(4326..4985)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     origin          5347..337
                     /label="p15A_origin"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttattaatca gataaaatat ttgctcatga gcccgaagtg
      361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
      421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc
      481 ttatcatcga tgcatctgac agctagctca gtcctaggta taatgctagc gagctcAGGA
      541 GGACATCATG ACAACAATGA CAGTACATAC AATGGGAGTA CATTATAAGT GGCAGATCCC
      601 CGAAGTCCTT CGGCAGCAAC TGTGGTTAGC ACATAACCTG CGGGAAGATC TGGTATCGCT
      661 TCAGTTAGCA TATGATGATG ATTTAAAGGC AATTTGGTCA TCGTATCCCG ACGTAGCACA
      721 GGCAGAAGAT ACAATGGCAG CAGCCGAAGC AGATGCAGTT GCACTGTCGG AACGGGTTAA
      781 ACAGGCACGG ATTGAAGCAC GGTCGAAAAA GATTTCGACA GAACTGACAC AACAGTTACG
      841 GGATGCAAAA AAACGGTTAA AAGATGCCCG GCAAGCACGG CGGGATGCAA TTGCAGTTGT
      901 AAAGGATGAC GCAGCCGAAC GGCGGAAGGC ACGGTCGGAT CAATTAGCAG CAGATCAGAA
      961 GGCACTGTAT GGGCAATATT GCCGAGATGG AGATCTGTAT TGGGCATCGT TTAATACAGT
     1021 ACTGGATCAC CATAAAACAG CCGTTAAGCG AATCGCAGCA CAACGGGCCT CAGGGAAACC
     1081 GGCAACTCTT CGACACCACC GGTTTGACGG ATCGGGAACA ATTGCAGTAC AGCTGCAGCG
     1141 GCAGGCAGGG GCACCGCCGC GGACACCCAT GGTCTTAGCA GATGAAGCAG GAAAATATCG
     1201 GAATGTACTT CATATCCCCG GGTGGACTGA TCCCGACGTA TGGGAACAGA TGACACGGTC
     1261 ACAATGCCGG CAATCGGGGC GGGTTACAGT ACGGATGCGG TGCGGATCGA CAGATGGACA
     1321 GCCCCAGTGG ATTGATCTAC CGGTACAAGT ACATCGCTGG TTACCGGCAG ATGCAGATAT
     1381 TACAGGAGCA GAATTAGTTG TCACACGGGT TGCAGGAATT TATCGGGCAA AACTGTGTGT
     1441 TACAGCACGG ATTGGGGATA CTGAACCCGT TACATCGGGG CCGACAGTAG CATTACATCT
     1501 GGGATGGCGC TCGACAGAAG AAGGAACAGC AGTAGCAACT TGGCGGTCAG ATGCCCCCCT
     1561 GGATATTCCC TTTGGACTAC GGACAGTAAT GCGGGTTGAC GCCGCAGGGA CATCGGGGAT
     1621 TATTGTAGTA CCCGCAACAA TTGAACGGCG GCTGACTCGG ACTGAAAATA TTGCATCGTC
     1681 GCGGTCGCTG GCACTAGACG CACTACGGGA TAAGGTTGTC GGGTGGCTAT CAGATAACGA
     1741 TGCCCCCACA TACCGGGATG CCCCGCTGGA AGCAGCCACT GTTAAGCAGT GGAAGTCACC
     1801 CCAGCGCTTT GCCTCGCTAG CACATGCATG GAAGGATAAT GGAACAGAAA TTTCGGATAT
     1861 TTTATGGGCA TGGTTTTCGT TAGATCGCAA ACAATGGGCA CAACAAGAAA ATGGGCGGCG
     1921 GAAAGCCTTA GGGCATCGGG ATGATTTATA TCGGCAGATT GCAGCAGTAA TTTCGGATCA
     1981 GGCAGGACAT GTTTTAGTTG ATGATACATC GGTAGCAGAA TTATCGGCAC GGGCAATGGA
     2041 ACGGACTGAA TTACCCACAG AAGTACAACA GAAAATTGAT CGGCGGCGGG ACCATGCAGC
     2101 ACCCGGGGGG CTACGGGCAT CGGTTGTAGC AGCAATGACA CGCGATGGAG TCCCCGTCAC
     2161 AATTGTTGCC GCCGCAGACT TTACTCGGAC TGCTTCGCGC GCCGGACATG TTAATCCCGC
     2221 CGATGATCGG TATCTGTCGA ATCCCGTACG GTGCGACGGA TGCGGGGCAA TGTATGATCA
     2281 AGATCGGTCG TTCGTTACTC TGATGCTGCG CGCAGCAACT GCCCCCTCGA ACCCCTAGaa
     2341 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
     2401 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     2461 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     2521 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2581 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     2641 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     2701 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     2761 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2881 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2941 acgctggtga aagtaaaaga tgctgaagat cagttggggc aaactattaa ctggcgaact
     3001 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3061 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3121 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3181 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3241 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3301 actttagatt gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3361 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3421 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3481 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     3541 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3601 cgttctttaa tagtggactc ttgttccaaa cttgaacaac actcaaccct atctcgggct
     3661 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3721 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt taaaaggatc
     3781 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3841 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3901 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3961 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4021 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4081 cctacatacc tcgctctgct aatcctgtta ccagtcaggc atttgagaag cacacggtca
     4141 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
     4201 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
     4261 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
     4321 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     4381 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
     4441 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     4501 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     4561 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     4621 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     4681 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     4741 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     4801 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     4861 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     4921 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     4981 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     5041 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     5101 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     5161 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     5221 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     5281 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     5341 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     5401 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     5461 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     5521 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     5581 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     5641 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     5701 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     5761 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     5821 ggtaggcagt tcgctccaag ctggactgt
//