LOCUS       pAAV-ADP-MCS-FLA        5861 bp DNA     circular SYN 21-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2022 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    156..1797
                     /label=ADP
     misc_feature    1798..1894
                     /label=MCS
     CDS             1895..1966
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    1988..2576
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2459..2470)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2608..3084
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3124..3264
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3339..3794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4076..4180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4181..5041
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5212..5800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttggga agtgtccagg gccatggggt
      181 cacaactaac agcccttgga aatgagcttg tgtccttaat cttcatgacc taacgtgatt
      241 tctctagaaa catcagtgca ttaacaggaa gacaagatgg aagatcatat tttggctctc
      301 cttccttggt gggttgacac tgctggtcct atccactagt aaaagcatga ctcttaggct
      361 ctgtgtggcc agtggaaggt ggcagttgga ggaagcagat gcttggccag cctttgcctg
      421 ggagcagtct agctctgagt gtcttattgg agcagctgct ggcatccaga gttctttttg
      481 gattcacgat ttaattcaaa agctttgtgc tcccgagaat cagctctggt ctttcaaaaa
      541 taagatgtga gtccgccgag aggctcccaa ggtattgcct tgccaactgc aagcctttta
      601 ggagcagttt agtgagtggt gactgctagt tgcagttggc tgttagccca gagctaataa
      661 tagatagaaa aggtatatac ttaaggagtc tggaaactga ggtttatcta ctcacagaaa
      721 atgagtttct aaaaaactag cttgaaactt acccagaaaa atcttagaac atggttctcc
      781 aatgtcaagg taagtgttct gtgacactgg gcttgaatta tgtagggacc acagatttta
      841 gaatttggac ccctgaactt gcttcacacc ccaccaggaa ccttcctgta caacagccct
      901 cagaattcat ctacatggtc ttttctcagt atgggatccg gtctagcaag tggagcacac
      961 cttctattgc ttaaagattt gtttatgtat atgggtattt tggctgcatg catatttgca
     1021 caccaaaaga aggcagcgga tcccatggaa ttactgtggg tgctgggaat tgaactcagg
     1081 acctctggaa gaatagccag tgctcttaac cactgagcca tgcctgcagt ccatctattt
     1141 tttattctag tacagcccct cttcattctt actgaaatag taatgcctga accacacagc
     1201 ttcacattta gttacaaaga aagagtggga gtatcatgtg acaattagtg ttgttgactc
     1261 tccaggacaa acttatggga aagggaggtc tcctgacccc tgaacaatca ttttacttga
     1321 ggataatttt cattgcactc agaaacatgc tgaattattg tccttaccct tgccccatct
     1381 cttgctctgg tagagaatgg ccaaagcctg gaaacaggat ggcttgacag aagctctact
     1441 tggcttccca gacccaagct ggattaaacc aggttcccta aggagtctta aggcagctgc
     1501 caggagcaag gggcccactc attggctatt ggccttgact gggttggcca atggtaagct
     1561 ggggtctgcc tgtccccatg agtaccagac taatgagacc tggccacttt ctcctcattt
     1621 ctgtctgtac gattgtcagt ggatctgacg acaccaaaag gtaagaacaa ttctatattc
     1681 tcggctggct gggtatgaat gcagaatccc acttgggctg tgttcagatt ttgctctctg
     1741 cagcagtgtg aaggtagatg ttgagaaaac tcaggccttg gaaacatggt tggggcaacc
     1801 ggtggtttag tgaaccgtca gatccgctag agatctgcgg ccgcagtact gctagcacta
     1861 gtgatatccg atcggtcgac gcccgggcct cgaggactac aaggatgacg atgacaagga
     1921 ttacaaagac gacgatgata aggactataa ggatgatgac gacaaataag atatcaagct
     1981 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2041 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2101 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2161 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2221 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2281 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2341 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2401 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2461 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2521 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2581 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2641 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2701 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2761 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2821 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2881 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2941 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3001 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3061 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     3121 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3181 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3241 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3301 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3361 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3421 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3481 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3541 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3601 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3661 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     3721 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3781 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3841 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3901 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3961 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4021 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4081 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4141 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4201 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4261 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4321 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4381 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4441 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4501 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4561 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4621 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4681 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4741 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4801 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4861 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4921 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4981 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5041 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5101 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5161 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5221 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5281 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5341 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5401 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5461 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5521 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5581 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5641 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5701 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5761 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5821 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
