LOCUS       Exported               12717 bp DNA     circular SYN 20-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12717)
  AUTHORS   James Lloyd
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12717
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    824..844
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    845..877
                     /label=B3RT
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(1239..1266)
                     /label=OCS_IntF1_R
     primer_bind     1261..1274
                     /label=OCS_IntF2_F
     misc_feature    1586..1618
                     /label=B3RT
                     /label=B3RT(1)
     misc_feature    1619..1653
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     1654..1682
                     /label=rLUC-IV2-PEST_CDS_BpiI_F
     CDS             join(1655..1684,1874..2866)
                     /codon_start=1
                     /label=R-LUC
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQGSRSDASEVTSTSSQSEVCTVETPGCVGS
                     "
     primer_bind     1682..1704
                     /label=IV2-GIB-B3o_F
     intron          1685..1873
                     /label=IV2 intron
     primer_bind     complement(1853..1873)
                     /label=IV2-GIB-B3o_R
     CDS             2777..2863
                     /label=PEST
     primer_bind     complement(2844..2871)
                     /label=rLUC-IV2-PEST_CDS_BpiI_R
     polyA_signal    2901..3074
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     3095..3119
                     /label=TCTPpro_BpiI_F
     promoter        3099..3256
                     /label=start of TCTP promoter
     promoter        3314..3328
                     /label=TSS
     primer_bind     complement(3374..3405)
                     /label=TCTPpro_BpiI_R
     5'UTR           3406..3461
                     /label=AtRbcS2B
     CDS             3463..5115
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3529..3547)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5135..5335
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     primer_bind     5356..5380
                     /label=TCTPpro_BpiI_F
     promoter        5360..5517
                     /label=start of TCTP promoter
     promoter        5575..5589
                     /label=TSS
     primer_bind     complement(5635..5666)
                     /label=TCTPpro_BpiI_R
     misc_feature    5678..5733
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     5734..5758
                     /label=B3o_ORF_BpiI_F
     CDS             5735..6537
                     /label=NLS-B3o
     primer_bind     6407..6427
                     /label=B3_seq_F
     misc_feature    6425..6427
                     /label=Flp intron insertion codon site
     primer_bind     6512..6555
                     /label=IV2-GIB-B3o_F
     primer_bind     complement(6512..6555)
                     /label=B3o-GIB-IV2_R
     misc_feature    6534..6535
                     /label=splice site
                     
     misc_feature    6536..6724
                     /label=potato_Intron_IV2
     primer_bind     complement(6704..6746)
                     /label=IV2-GIB-B3o_R
     primer_bind     6704..6746
                     /label=B3o-GIB-IV2_F
     CDS             6722..7651
                     /label=NLS-B3o
     misc_feature    complement(6725)
                     /label=splice site
     C_region        7199..7651
                     /label=Extention relative to Flp
     primer_bind     complement(7631..7656)
                     /label=B3o_ORF_BpiI_R
     polyA_signal    7686..7859
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7930..7951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7966..7996
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8004..8020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8009..8031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8028..8044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8028..8044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8040..8363
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8057..8113
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8114..8131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8114..8130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8123..8145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8339..8358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8495..8519
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8607..9317
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9338..9442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9443..10237
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10408..10996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10897..10916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11332..12480
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12626..12650
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaaccca
     1621 acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt atgatcctga
     1681 gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt aattagtagt
     1741 aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt
     1801 tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt
     1861 tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa gatgtaaaca
     1921 gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg ctgagaatgc
     1981 agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg ttgtgcctca
     2041 cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa agtctggtaa
     2101 atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag cttggttcga
     2161 acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg cttgcttggc
     2221 attccattac tcatacgaac accaagataa gatcaaagct atagttcatg cagagagtgt
     2281 tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata ttgctttgat
     2341 caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg aaactatgct
     2401 tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc tcgaaccttt
     2461 caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa taccattagt
     2521 taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt accttagggc
     2581 atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca gtaatgctat
     2641 agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag gattgcattt
     2701 ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg ttgaaagagt
     2761 gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa caagctccca
     2821 gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt ctctagctag
     2881 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     2941 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     3001 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     3061 actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa tccccacccg
     3121 ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat ttaaaaaaaa
     3181 ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta atttaccgag
     3241 aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac caaacttagg
     3301 acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg attttcgttt
     3361 attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa caagtaagta
     3421 agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga cgccaaaaac
     3481 ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg agagcaactg
     3541 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     3601 atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct
     3661 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     3721 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     3781 gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg
     3841 tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc
     3901 cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     3961 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt
     4021 gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag
     4081 ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt
     4141 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     4201 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     4261 agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg
     4321 ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta
     4381 tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg
     4441 gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca
     4501 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     4561 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag
     4621 agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa
     4681 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     4741 gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga
     4801 tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac
     4861 gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     4921 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     4981 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     5041 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     5101 aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca atttatgtat
     5161 tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt
     5221 tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat
     5281 aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc aagatcgcta
     5341 ctagaattcg agctcggagc caacactcga atccccaccc gttggaccaa acccggctca
     5401 ttaagcgtcg gttcagattt atttccttta tttaaaaaaa aggaaagggt aaaaaataga
     5461 aaattggaaa cagttaaagc ccaaaattgt aatttaccga gaattgtaaa tttacctgaa
     5521 aaccctacgc tatagtttcg actataaata ccaaacttag gacctcactt cagaatcccc
     5581 tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt tattcgcatc catcggagag
     5641 agaaaacaat caataagcga cctactgtat ttttacaaca attaccaaca acaacaaaca
     5701 acaaacaaca ttacaattac tatttacaat tacaatgccc aagaaaaagc ggaaggtgtc
     5761 cagctacatg gacctggtgg acgacgagcc cgccaccctg taccacaagt tcgtggaatg
     5821 cctgaaggcc ggcgagaact tctgcggcga taagctgagc ggcatcatca ccatggccat
     5881 tctgaaggcc atcaaggccc tgaccgaagt gaagaaaacc accttcaaca agtacaagac
     5941 caccatcaag cagggcctgc agtacgacgt gggcagcagc accatcagct tcgtgtacca
     6001 cctgaaggac tgcgacgagc tgagcagagg cctgagcgac gccttcgagc cctacaagtt
     6061 caagatcaag agcaacaaag aggccaccag cttcaagacc ctgttcaggg gccctagctt
     6121 cggcagccag aagaactggc ggaagaaaga ggtggaccgc gaggtggaca acctgttcca
     6181 cagcaccgag acagacgaga gcatcttcaa gttcatcctg aacaccctgg acagcatcga
     6241 aacccagacc aacaccgacc ggcagaaaac cgtgctgacc tttatcctgc tgatgacctt
     6301 cttcaactgc tgccggaaca acgacctgat gaacgtggac cccagcacct tcaagatcgt
     6361 gaagaacaag tttgtgggct acctgctgca ggctgaagtg aagcagacca agaccagaaa
     6421 gagccggaat atcttcttct tccccatccg ggaaaaccgc ttcgacctgt tcctggccct
     6481 gcacgacttc ttcagaacct gccagcccac ccccaagagc agactgagcg atcaggtaag
     6541 tttctgcttc tacctttgat atatatataa taattatcat taattagtag taatataata
     6601 tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt
     6661 tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt
     6721 gcaggtgtcc gagcagaagt ggcagctgtt ccgggacagc atggtcatcg actacaaccg
     6781 gttctttcgg aagttccccg ccagccccat cttcgccatt aagcacggcc ccaagtccca
     6841 cctgggccgg catctgatga acagctttct gcacaagaac gagctggaca gctgggccaa
     6901 cagcctgggc aattggagca gctcccagaa ccagagagag agcggcgcca gactgggcta
     6961 cacacacggc ggaagagatc tgccccagcc cctgtttggc ttcctggccg gatactgcgt
     7021 gcggaacgaa gagggccaca tcgtgggcct gggcctggaa aaggacatca acgatctgtt
     7081 cgacggcatc atggaccccc tgaacgagaa agaggacacc gagatctgcg agagctacgg
     7141 cgagtgggcc aagattgtgt ccaaggacgt gctgatcttc ctgaagagat accacagcaa
     7201 gaacgcctgt cggagatacc agaacagcac cctgtatgcc cggaccttcc tgaaaaccga
     7261 gagcgtgacc ctgagcggct ccaagggcag cgaggaacct tctagccctg tgcggatccc
     7321 catcctgagc atgggaaagg ccagcccctc cgagggaaga aagctgagag ccagcgagca
     7381 cgccaacgac gacaacgaga tcgagaagat cgacagcgac agcagccaga gcgaagagat
     7441 ccctatcgag atgagcgact ccgaggacga gacaaccgcc agcaacatca gcggcatcta
     7501 cctggacatg agcaaggcca actccaacgt ggtgtacagc ccccctagcc agacaggcag
     7561 agctgctggc gccggaagaa aaagaggcgt gggaggcaga cggaccgtgg aaagcaagcg
     7621 gagaagagtg ctggccccca tcaaccggtg agcttctcta gctagagtcg atcgacaagc
     7681 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
     7741 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     7801 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
     7861 gctttactga gaccgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7921 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7981 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8041 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
     8101 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8161 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8221 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     8281 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     8341 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8401 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acggtctcag
     8461 ggacacgaag tgatccgttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     8521 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     8581 ccgttcgtcc atttgtatgt gccagccgcc tttgcgacgc tcaccgggct ggttgccctc
     8641 gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg
     8701 tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     8761 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     8821 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     8881 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     8941 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     9001 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     9061 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     9121 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     9181 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     9241 gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     9301 caccccaaaa atggcagcgc tggccaattc ccgagtgcgc ggaaccccta tttgtttatt
     9361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9421 ataatattga aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact
     9481 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     9541 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     9601 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     9661 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     9721 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     9781 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     9841 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     9901 tgtggattgc gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga
     9961 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
    10021 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
    10081 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg
    10141 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
    10201 aataaaatat tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata
    10261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    10561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg cagatcctag
    11041 atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt
    11101 tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg
    11161 cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac
    11221 ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa
    11281 taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg
    11341 acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat
    11401 gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc
    11461 ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc
    11521 cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg
    11581 gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga
    11641 aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg
    11701 aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg
    11761 ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc
    11821 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
    11881 gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
    11941 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac
    12001 gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa
    12061 tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg
    12121 gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt
    12181 tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa
    12241 ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc
    12301 agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg
    12361 gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga
    12421 ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag
    12481 ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc ctgctcggat ctgttggacc
    12541 ggacagtagt catggttgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    12601 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
    12661 ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggttga acactct
//
