LOCUS       pcDNA3-mCherry-T       11589 bp DNA     circular SYN 20-SEP-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Jin Zhang Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 20, 2022 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /label=6His
                     /note="color: #008000"
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /note="color: #cc99b2"
                     /translation="MASMTGGQQMG"
     CDS             979..1002
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /note="This feature has 2 segments:
                        1:  979 ..  987 / #cc99b2
                        2:  988 .. 1002 / #cc99b2 / enterokinase site
                     Cleavage site after base 1002"
                     /translation="DLYDDDDK"
     CDS             1009..1716
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1738..7086
                     /codon_start=1
                     /label=TSC2 isoform5 (S939A,T1439A)
                     /note="color: #993366"
                     /translation="AKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILR
                     ELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALL
                     KAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFV
                     LQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLD
                     AVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMED
                     RAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLS
                     ITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEF
                     HGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRG
                     AVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEG
                     CHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYT
                     LPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDG
                     VVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQ
                     CLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAP
                     EGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSV
                     EMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAIS
                     LPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKD
                     SFRARSTALNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSR
                     IQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGE
                     FLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEA
                     PAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPE
                     KASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNM
                     PLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTDSAVVMEEGSPGEV
                     PVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEG
                     GRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGE
                     SAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYAISDSAPSRRGKRVERDALKSRAT
                     ASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHK
                     IAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGED
                     GQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTI
                     KGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANM
                     ASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQT
                     PAEPTPGYEVGQRKRLISSVEDFTEFV"
     CDS             7093..7122
                     /codon_start=1
                     /label=NLS-Targeting
                     /note="color: #800040"
                     /translation="PKKKRKVEDA"
     promoter        complement(7141..7159)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    7185..7409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7455..7883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7897..8227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8078..8213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             8294..9088
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    9262..9383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9432..9448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9456..9472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9480..9510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9480 .. 9486 / #ffffff / -10
                        2: 9487 .. 9504 / #ffffff
                        3: 9505 .. 9510 / #ffffff / -35"
     protein_bind    9525..9546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(9834..10422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10593..11453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10593 .. 11384 / #ccffcc
                        2: 11385 .. 11453 / #ccffcc / signal sequence
                     Cleavage site after base 11384"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11454..11558)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1081 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1141 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1201 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1261 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1321 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1381 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1441 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1501 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1561 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1621 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1681 cgccactcca ccggcggcat ggacgagctg tacaagcgca tgcatgcaag ggatccagcc
     1741 aaaccaacaa gcaaagattc aggcttgaag gagaagttta agattctgtt gggactggga
     1801 acaccgaggc caaatcccag gtctgcagag ggtaaacaga cggagtttat catcaccgcg
     1861 gaaatactga gagaactgag catggaatgt ggcctcaaca atcgcatccg gatgataggg
     1921 cagatttgtg aagtcgcaaa aaccaagaaa tttgaagagc acgcagtgga agcactctgg
     1981 aaggcggtcg cggatctgtt gcagccggag cggccgctgg aggcccggca cgcggtgctg
     2041 gctctgctga aggccatcgt gcaggggcag ggcgagcgtt tgggggtcct cagagccctc
     2101 ttctttaagg tcatcaagga ttacccttcc aacgaagacc ttcacgaaag gctggaggtt
     2161 ttcaaggccc tcacagacaa tgggagacac atcacctact tggaggaaga gctggctgac
     2221 tttgtcctgc agtggatgga tgttggcttg tcctcggaat tccttctggt gctggtgaac
     2281 ttggtcaaat tcaatagctg ttacctcgac gagtacatcg caaggatggt tcagatgatc
     2341 tgtctgctgt gcgtccggac cgcgtcctct gtggacatag aggtctccct gcaggtgctg
     2401 gacgccgtgg tctgctacaa ctgcctgccg gctgagagcc tcccgctgtt catcgttacc
     2461 ctctgtcgca ccatcaacgt caaggagctc tgcgagcctt gctggaagct gatgcggaac
     2521 ctccttggca cccacctggg ccacagcgcc atctacaaca tgtgccacct catggaggac
     2581 agagcctaca tggaggacgc gcccctgctg agaggagccg tgttttttgt gggcatggct
     2641 ctctggggag cccaccggct ctattctctc aggaactcgc cgacatctgt gttgccatca
     2701 ttttaccagg ccatggcatg tccgaacgag gtggtgtcct atgagatcgt cctgtccatc
     2761 accaggctca tcaagaagta taggaaggag ctccaggtgg tggcgtggga cattctgctg
     2821 aacatcatcg aacggctcct tcagcagctc cagaccttgg acagcccgga gctcaggacc
     2881 atcgtccatg acctgttgac cacggtggag gagctgtgtg accagaacga gttccacggg
     2941 tctcaggaga gatactttga actggtggag agatgtgcgg accagaggcc tgagtcctcc
     3001 ctcctgaacc tgatctccta tagagcgcag tccatccacc cggccaagga cggctggatt
     3061 cagaacctgc aggcgctgat ggagagattc ttcaggagcg agtcccgagg cgccgtgcgc
     3121 atcaaggtgc tggacgtgct gtcctttgtg ctgctcatca acaggcagtt ctatgaggag
     3181 gagctgatta actcagtggt catctcgcag ctctcccaca tccccgagga taaagaccac
     3241 caggtccgaa agctggccac ccagttgctg gtggacctgg cagagggctg ccacacacac
     3301 cacttcaaca gcctgctgga catcatcgag aaggtgatgg cccgctccct ctccccaccc
     3361 ccggagctgg aagaaaggga tgtggccgca tactcggcct ccttggagga tgtgaagaca
     3421 gccgtcctgg ggcttctggt catccttcag accaagctgt acaccctgcc tgcaagccac
     3481 gccacgcgtg tgtatgagat gctggtcagc cacattcagc tccactacaa gcacagctac
     3541 accctgccaa tcgcgagcag catccggctg caggcctttg acttcctgtt gctgctgcgg
     3601 gccgactcac tgcaccgcct gggcctgccc aacaaggatg gagtcgtgcg gttcagcccc
     3661 tactgcgtct gcgactacat ggagccagag agaggctctg agaagaagac cagcggcccc
     3721 ctttctcctc ccacagggcc tcctggcccg gcgcctgcag gccccgccgt gcggctgggg
     3781 tccgtgccct actccctgct cttccgcgtc ctgctgcagt gcttgaagca ggagtctgac
     3841 tggaaggtgc tgaagctggt tctgggcagg ctgcctgagt ccctgcgcta taaagtgctc
     3901 atctttactt ccccttgcag tgtggaccag ctgtgctctg ctctctgctc catgctttca
     3961 ggcccaaaga cactggagcg gctccgaggc gccccagaag gcttctccag aactgacttg
     4021 cacctggccg tggttccagt gctgacagca ttaatctctt accataacta cctggacaaa
     4081 accaaacagc gcgagatggt ctactgcctg gagcagggcc tcatccaccg ctgtgccagc
     4141 cagtgcgtcg tggccttgtc catctgcagc gtggagatgc ctgacatcat catcaaggcg
     4201 ctgcctgttc tggtggtgaa gctcacgcac atctcagcca cagccagcat ggccgtccca
     4261 ctgctggagt tcctgtccac tctggccagg ctgccgcacc tctacaggaa ctttgccgcg
     4321 gagcagtatg ccagtgtgtt cgccatctcc ctgccgtaca ccaacccctc caagtttaat
     4381 cagtacatcg tgtgtctggc ccatcacgtc atagccatgt ggttcatcag gtgccgcctg
     4441 cccttccgga aggattttgt ccctttcatc actaagggcc tgcggtccaa tgtcctcttg
     4501 tcttttgatg acacccccga gaaggacagc ttcagggccc ggagtactgc tctcaacgag
     4561 agacccaaga gtctgaggat agccagaccc cccaaacaag gcttgaataa ctctccaccc
     4621 gtgaaagaat tcaaggagag ctctgcagcc gaggccttcc ggtgccgcag catcagtgtg
     4681 tctgaacatg tggtccgcag caggatacag acgtccctca ccagtgccag cttggggtct
     4741 gcagatgaga actccgtggc ccaggctgac gatagcctga aaaacctcca cctggagctc
     4801 acggaaacct gtctggacat gatggctcga tacgtcttct ccaacttcac ggctgtcccg
     4861 aagaggtctc ctgtgggcga gttcctccta gcgggtggca ggaccaaaac ctggctggtt
     4921 gggaacaagc ttgtcactgt gacgacaagc gtgggaaccg ggacccggtc gttactaggc
     4981 ctggactcgg gggagctgca gtccggcccg gagtcgagct ccagccccgg ggtgcatgtg
     5041 agacagacca aggaggcgcc ggccaagctg gagtcccagg ctgggcagca ggtgtcccgt
     5101 ggggcccggg atcgggtccg ttccatgtcg gggggccatg gtcttcgagt tggcgccctg
     5161 gacgtgccgg cctcccagtt cctgggcagt gccacttctc caggaccacg gactgcacca
     5221 gccgcgaaac ctgagaaggc ctcagctggc acccgggttc ctgtgcagga gaagacgaac
     5281 ctggcggcct atgtgcccct gctgacccag ggctgggcgg agatcctggt ccggaggccc
     5341 acagggaaca ccagctggct gatgagcctg gagaacccgc tcagcccttt ctcctcggac
     5401 atcaacaaca tgcccctgca ggagctgtct aacgccctca tggcggctga gcgcttcaag
     5461 gagcaccggg acacagccct gtacaagtca ctgtcggtgc cggcagccag cacggccaaa
     5521 ccccctcctc tgcctcgctc caacacagac tccgccgtgg tcatggagga gggaagtccg
     5581 ggcgaggttc ctgtgctggt ggagccccca gggttggagg acgttgaggc agcgctaggc
     5641 atggacaggc gcacggatgc ctacagcagg tcgtcctcag tctccagcca ggaggagaag
     5701 tcgctccacg cggaggagct ggttggcagg ggcatcccca tcgagcgagt cgtctcctcg
     5761 gagggtggcc ggccctctgt ggacctctcc ttccagccct cgcagcccct gagcaagtcc
     5821 agctcctctc ccgagctgca gactctgcag gacatcctcg gggaccctgg ggacaaggcc
     5881 gacgtgggcc ggctgagccc tgaggttaag gcccggtcac agtcagggac cctggacggg
     5941 gaaagtgctg cctggtcggc ctcgggcgaa gacagtcggg gccagcccga gggtcccttg
     6001 ccttccagct ccccccgctc gcccagtggc ctccggcccc gaggttacgc gatctccgac
     6061 tcggccccat cacgcagggg caagagagta gagagggacg ccttaaagag cagagccaca
     6121 gcctccaatg cagagaaagt gccaggcatc aaccccagtt tcgtgttcct gcagctctac
     6181 cattccccct tctttggcga cgagtcaaac aagccaatcc tgctgcccaa tgagtcacag
     6241 tcctttgagc ggtcggtgca gctcctcgac cagatcccat catacgacac ccacaagatc
     6301 gccgtcctgt atgttggaga aggccagagc aacagcgagc tcgccatcct gtccaatgag
     6361 catggctcct acaggtacac ggagttcctg acgggcctgg gccggctcat cgagctgaag
     6421 gactgccagc cggacaaggt gtacctggga ggcctggacg tgtgtggtga ggacggccag
     6481 ttcacctact gctggcacga tgacatcatg caagccgtct tccacatcgc caccctgatg
     6541 cccaccaagg acgtggacaa gcaccgctgc gacaagaagc gccacctggg caacgacttt
     6601 gtgtccattg tctacaatga ctccggtgag gacttcaagc ttggcaccat caagggccag
     6661 ttcaactttg tccacgtgat cgtcaccccg ctggactacg agtgcaacct ggtgtccctg
     6721 cagtgcagga aagacatgga gggccttgtg gacaccagcg tggccaagat cgtgtctgac
     6781 cgcaacctgc ccttcgtggc ccgccagatg gccctgcacg caaatatggc ctcacaggtg
     6841 catcatagcc gctccaaccc caccgatatc tacccctcca agtggattgc ccggctccgc
     6901 cacatcaagc ggctccgcca gcggatctgc gaggaagccg cctactccaa ccccagccta
     6961 cctctggtgc accctccgtc ccatagcaaa gcccctgcac agactccagc cgagcccaca
     7021 cctggctatg aggtgggcca gcggaagcgc ctcatctcct cggtggagga cttcaccgag
     7081 tttgtggaat tccccaaaaa gaagagaaag gtggaagacg cataatctag agggccctat
     7141 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     7201 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     7261 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     7321 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7381 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7441 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7501 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7561 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     7621 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7681 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7741 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7801 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     7861 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7921 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7981 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8041 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8101 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8161 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8221 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     8281 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     8341 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     8401 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     8461 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     8521 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     8581 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     8641 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     8701 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     8761 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8821 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8881 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     8941 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     9001 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     9061 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     9121 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9181 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     9241 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9361 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9601 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10021 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10261 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11581 cctgacgtc
//
