LOCUS       Exported                7681 bp DNA     circular SYN 21-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(245..349)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      376..804
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(835..989)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     enhancer        1149..1528
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        1530..1729
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1763..4807
                     /codon_start=1
                     /label=BALF5 (Y753F)
                     /translation="MSGGLFYNPFLRPNKGLLKKPDKEYLRLIPKCFQTPGAAGVVDVR
                     GPQPPLCFYQDSLTVVGGDEDGKGMWWRQRAQEGTARPEADTHGSPLDFHVYDILETVY
                     THEKCAVIPSDKQGYVVPCGIVIKLLGRRKADGASVCVNVFGQQAYFYASAPQGLDVEF
                     AVLSALKASTFDRRTPCRVSVEKVTRRSIMGYGNHAGDYHKITLSHPNSVCHVATWLQD
                     KHGCRIFEANVDATRRFVLDNDFVTFGWYSCRRAIPRLQHRDSYAELEYDCEVGDLSVR
                     REDSSWPSYQALAFDIECLGEEGFPTATNEADLILQISCVLWSTGEEAGRYRRILLTLG
                     TCEDIEGVEVYEFPSELDMLYAFFQLIRDLSVEIVTGYNVANFDWPYILDRARHIYSIN
                     PASLGKIRAGGVCEVRRPHDAGKGFLRANTKVRITGLIPIDMYAVCRDKLSLSDYKLDT
                     VARHLLGAKKEDVHYKEIPRLFAAGPEGRRRLGMYCVQDSALVMDLLNHFVIHVEVAEI
                     AKIAHIPCRRVLDDGQQIRVFSCLLAAAQKENFILPMPSASDRDGYQGATVIQPLSGFY
                     NSPVLVVDFASLYPSIIQAHNLCYSTMITPGEEHRLAGLRPGEDYESFRLTGGVYHFVK
                     KHVHESFLASLLTSWLAKRKAIKKLLAACEDPRQRTILDKQQLAIKCTCNAVYGFTGVA
                     NGLFPCLSIAETVTLQGRTMLERAKAFVEALSPANLQALAPSPDAWAPLNPEGQLRVIF
                     GDTDSLFIECRGFSESETLRFADALAAHTTRSLFVAPISLEAEKTFSCLMLITKKRYVG
                     VLTDGKTLMKGVELVRKTACKFVQTRCRRVLDLVLADARVKEAASLLSHRPFQESFTQG
                     LPVGFLPVIDILNQAYTDLREGRVPMGELCFSTELSRKLSAYKSTQMPHLAVYQKFVER
                     NEELPQIHDRIQYVFVEPKGGVKGARKTEMAEDPAYAERHGVPVAVDHYFDKLLQGAAN
                     ILQCLFDNNSGAALSVLQNFTARPPF"
     CDS             4823..4846
                     /codon_start=1
                     /label=FLAG
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="DYKDDDDK"
     polyA_signal    4853..5475
                     /label=hGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        5517..5588
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.6"
                     /note="/match_length=24.2"
                     /note="/other=promoter"
     promoter        5615..5825
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        complement(5872..5890)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(5928..5944)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(5952..5982)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(5997..6018)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(6031..6123)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(6306..6894)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(7065..7681,1..244))
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
       61 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
      121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      361 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      421 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      481 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      541 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      601 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      661 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      721 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      781 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc
      841 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      901 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      961 gtcacgacgt tgtaaaacga cggccagtgc caagctgatc tatacattga atcaatattg
     1021 gcaattagcc atattagtca ttggttatat agcataaatc aatattggct attggccatt
     1081 gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc
     1141 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     1201 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1261 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1321 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1381 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
     1441 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
     1501 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
     1561 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1621 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
     1681 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     1741 gaaccgtcag aattaagctt cgatgtctgg gggactcttc tataaccctt tcctaagacc
     1801 taataaaggc cttctgaaaa agcctgacaa ggagtacctg cgtctcattc ccaagtgttt
     1861 ccagacacca ggcgccgcag gggtggtgga tgtgcggggg cctcagcccc ccctgtgctt
     1921 ctaccaagac tccctgacgg tggtgggggg tgacgaggat ggaaagggca tgtggtggcg
     1981 ccagcgtgcc caagagggca cggcaaggcc ggaggcagac acccacggaa gccctctgga
     2041 cttccatgtc tacgacatac tcgagacggt gtacacgcac gagaaatgcg ccgtcattcc
     2101 atcggataaa caggggtatg tggtgccatg tggcatcgtc atcaagctac tgggccggcg
     2161 caaggccgat ggggccagcg tgtgtgtgaa cgtgtttggg cagcaggcct acttctacgc
     2221 cagcgcgcct cagggtctgg acgtggagtt tgcagtcctc agcgccctca aggccagcac
     2281 cttcgaccgc aggaccccct gccgggtctc ggtggagaag gtcacgcgcc gttccattat
     2341 gggctacggc aaccatgccg gcgactacca caagatcacc ctctcccatc ccaacagtgt
     2401 gtgtcacgtg gccacgtggc tgcaagacaa gcacgggtgt cggatctttg aggccaacgt
     2461 ggatgccacg cgccgctttg tcctggacaa tgactttgtc acctttggct ggtacagctg
     2521 ccgccgcgcc atcccccgcc tccagcaccg ggactcgtac gccgagctcg agtacgactg
     2581 tgaggtgggc gacctctcgg tccggcgtga agacagctcc tggccctcct accaggccct
     2641 ggccttcgat atcgagtgtc tgggggagga gggcttcccc acggccacca acgaggctga
     2701 cctgatcctg cagatatcct gcgtcctctg gtcgacaggg gaggaggccg ggcgctatag
     2761 gcgcatcctg ctgacgctgg gcacctgcga agacatagag ggggttgagg tctacgagtt
     2821 cccatcggag ctggacatgc tctacgcctt cttccagctc atcagagacc tcagcgtgga
     2881 gattgtgacc ggctacaacg tggccaactt tgactggccc tacattctgg acagagccag
     2941 gcacatctac agcatcaacc cagcctctct gggcaaaatt agggctgggg gcgtctgcga
     3001 ggtcaggcga ccccatgatg cgggcaaggg cttcttgcgg gccaacacca aggtccgcat
     3061 caccggcctc atccccatcg acatgtacgc cgtgtgccgg gacaagctca gcctctcaga
     3121 ctacaagctg gacacagtag ccaggcacct actgggggcc aagaaggagg atgtgcatta
     3181 caaggagatt cctcgcctct ttgcagcggg ccccgagggg cgcaggcggc tcggcatgta
     3241 ctgcgtgcag gactcggccc tggtcatgga tctgctaaac catttcgtga tccacgtgga
     3301 ggtggcagag attgccaaga tcgctcacat cccctgcagg cgggtgctgg acgatgggca
     3361 gcagatccgc gtgttctcct gcctcctggc ggccgcccaa aaggaaaact ttatcctgcc
     3421 catgccctcg gcctctgacc gggacggcta ccagggggcc accgtcatcc agcccctgtc
     3481 cggattctac aactccccgg ttctggtggt ggactttgcc agcctctacc cgagcatcat
     3541 tcaggctcat aatctctgtt attctaccat gataacgccg ggagaagagc acaggctagc
     3601 cggcctgcgc ccgggagaag actatgagtc cttcaggctc acggggggcg tctaccactt
     3661 tgtaaagaag cacgtgcacg agtccttctt ggctagtctg ttgacctcct ggctggccaa
     3721 gcgcaaggcc atcaagaagc tgctggcggc ctgcgaggat ccgcgccaaa ggaccatcct
     3781 cgacaagcag cagctggcca tcaagtgcac gtgcaacgcc gtctacggct tcaccggggt
     3841 ggccaacggc ctctttccct gcctctccat cgccgagacg gtgacgctgc agggccgcac
     3901 gatgttggaa cgagctaaag ctttcgttga agctctgagc cctgctaatc tgcaggctct
     3961 ggccccctcc ccggacgcct gggcgcccct caaccccgag ggccagcttc gagtcatctt
     4021 cggggacacg gactcgctgt ttatcgagtg ccgggggttt tcagagagcg agaccctgcg
     4081 ctttgccgat gccctggccg cccacaccac ccggagcctg tttgtggccc ccatctccct
     4141 ggaggccgag aagaccttct cctgcctgat gctgattaca aagaagagat atgtgggggt
     4201 gctgacggac ggcaagaccc tgatgaaggg ggtggagctc gtccggaaga cggcctgcaa
     4261 gtttgtgcag acacgctgcc ggcgcgtgct cgacctggtg ctggcggatg cccgggtaaa
     4321 ggaggcggcc agcctcctct cccaccggcc cttccaagag tcatttacac aagggctacc
     4381 tgtgggcttt ttgcccgtca ttgacatcct aaaccaggcc tacacagacc tccgtgaagg
     4441 cagggtcccc atgggggagc tctgcttttc aacggagctc agccgcaagc tctcagccta
     4501 caagagcacc cagatgcctc acctggccgt ctaccagaag ttcgtcgagc gcaacgagga
     4561 actgccccag atccacgacc gcatccagta cgtctttgtg gagcccaagg ggggagtgaa
     4621 gggggcgaga aagacggaga tggccgagga cccggcctac gccgagcggc acggcgttcc
     4681 cgtggccgtg gatcattatt tcgacaagct gctccaagga gcggccaaca tcctccagtg
     4741 cctctttgat aacaactccg gggccgccct ctccgtcctc cagaatttta cagcccggcc
     4801 accattcata gatcgccggg ctgattacaa ggacgacgat gacaagtagg ttgggtggca
     4861 tccctgtgac ccctccccag tgcctctcct ggccccggaa gttgccactc cagtgcccac
     4921 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     4981 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     5041 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     5101 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     5161 ccaggcatgc atgaccgggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     5221 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     5281 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa
     5341 ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc
     5401 ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta
     5461 gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg
     5521 tggaaagtcc ccgggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5581 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5641 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5701 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5761 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5821 aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt ccctatagtg
     5881 agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5941 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6001 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6061 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7681 a
//
