LOCUS       pAcGFP1-N1_GS_CAHS8        5361 bp    DNA     circular UNA 28-FEB-2020
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...ie-ab7u4zz
VERSION     urn.local...ie-ab7u4zz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..589
                     /created_by="User"
                     /label="CMV"
     primer          492..511
                     /created_by="kunieda"
                     /label="pXFP-seq-Fwd"
     primer          519..539
                     /created_by="kunieda"
                     /label="CMV-Fwd"
     primer          537..555
                     /created_by="User"
                     /label="seq-primer-CMV"
     primer          complement(568..596)
                     /created_by="kunieda"
                     /modified_by="kunieda"
                     /label="N_V_iF"
     primer          complement(576..599)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="SDM_CAHS8_iF"
     primer          582..621
                     /created_by="kunieda"
                     /label="N_CAHS8_F"
     misc_feature    591..>592
                     /created_by="User"
                     /modified_by="kunieda"
                     /label="MCS"
     motif           591..592
                     /created_by="kunieda"
                     /label="Kozak-N"
     feature         593..1262
                     /created_by="kunieda"
                     /label="insert"
     misc_feature    592^593
                     /Original_Bases="TAGCGCTACCGGACTCAGATCTCGAGCTCAAGCTTCGAATT
                     CTGCAGTCGACGGTACCGCGGGCCCGGGATCCACCGGTCGC"
                     /label="TAGCGCTACCGGACTCAGATCTCGAGCTCAAGCTTCGAATTCTGCAGTCG
                     ACGGTACCGCGGGCCCGGGATCCACCGGTCGC"
                     /note="Geneious type: Editing History Deletion"
     motif           593..596
                     /created_by="kunieda"
                     /label="Kozak_C"
     CDS             597..>1262
                     /created_by="kunieda"
                     /label="RvCAHS8"
     motif           684..746
                     /created_by="saibou"
                     /label="motif-5"
     primer          684..707
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="SDM_CAHS8_M5_iR"
     primer          complement(724..746)
                     /created_by="saibou"
                     /label="CAHS8_C_SDM_M5_iF"
     motif           759..821
                     /created_by="saibou"
                     /label="motif-3"
     primer          759..779
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="SDM_CAHS8_M3_iR"
     primer          complement(804..821)
                     /created_by="saibou"
                     /label="CAHS8_C_SDM_M3_iF"
     motif           831..953
                     /created_by="saibou"
                     /label="motif-4"
     primer          831..852
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="SDM_CAHS8_M4_iR"
     primer          complement(927..953)
                     /created_by="saibou"
                     /label="CAHS8_C_SDM_M4_iF"
     motif           957..1106
                     /created_by="saibou"
                     /label="motif-1"
     primer          957..978
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="SDM_CAHS8_M1_iR"
     primer          complement(1083..1106)
                     /created_by="saibou"
                     /label="CAHS8_C_SDM_M1_iF"
     motif           1107..1229
                     /created_by="saibou"
                     /label="motif-2"
     primer          1107..1126
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="SDM_CAHS8_M2_iR"
     primer          complement(1205..1229)
                     /created_by="saibou"
                     /label="CAHS8_C_SDM_M2_iF"
     primer          complement(1238..1277)
                     /created_by="kunieda"
                     /modified_by="kunieda"
                     /label="N_CAHS8_GS_R"
     linker          1263..1307
                     /created_by="kunieda"
                     /label="pBS Coupler segment"
     misc_feature    1262^1263
                     /Original_Bases="TAA"
                     /label="TAA"
                     /note="Geneious type: Editing History Deletion"
     primer          1263..1287
                     /created_by="kunieda"
                     /label="N_V_iR"
     primer          1263..1283
                     /created_by="saibou"
                     /label="C_SDM_iR"
     primer          complement(1268..1307)
                     /created_by="kunieda"
                     /modified_by="kunieda"
                     /label="pBSC_pR"
     primer          1293..1332
                     /created_by="kunieda"
                     /modified_by="kunieda"
                     /label="pAcGFP1-N1-iR"
     CDS             1308..2027
                     /created_by="User"
                     /label="AcGFP"
     primer          complement(1315..1335)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="seq-AcGFP1-N1-Rev"
     primer          complement(2008..2044)
                     /created_by="User"
                     /label="AcGFP1-CMV-R"
     primer          complement(2055..2074)
                     /created_by="User"
                     /label="seq-primer-FPend"
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gccaccatgt
      601 ctcagcgagc ttcggaacac tcatcatcca gccaccagtc gtctggccag tctggacagt
      661 cgagccagca cgtccaagag cagaacacca gctacacaca caccgatgtc cgcaccaacg
      721 ttcccaatgt ccctcctcca gtcatctcga ccagcgctgc tggtctcagt gagcagatgg
      781 tcggtcaagg tttcaccgcc tcggctgcgc gagtctccgg ccagtccacc gaagcccatg
      841 tccaaatgac ccctgagatg gaagccgagg cccgcaagga ccgcgaacga tacgagcgcg
      901 aactggaagc catcaaccaa cgccaccaga aggatattga aggcaagacc gaggcttacc
      961 gaaagcaagc cgaagctgag gcggaacgtc ttcgacggga actagagaag caacatcaac
     1021 gagacgttga gttccgcaag tccctggttc aagggacaat cgacaaccag aagaaacagg
     1081 ttgagctgga ggctatgatg gccaagcgcg agttggaccg agaagccaag ctggctcaag
     1141 atgctctcga gaagagcaaa atggccaccg atgctcaagt cacgttcgag tcagcagtag
     1201 gccacacgat ttctggtagc accaccgtgt cccagtccga gaaagtatcc cagtccaaga
     1261 agggtggagg cggttcaggc ggaggtggct ctggcggtgg cggatcgatg gtgagcaagg
     1321 gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg
     1381 gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1441 tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtgaccaccc
     1501 tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct
     1561 tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg
     1621 gcaactacaa gtcgcgcgcc gaggtgaagt tcgagggcga taccctggtg aatcgcatcg
     1681 agctgaccgg caccgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca
     1741 actacaacgc ccacaatgtg tacatcatga ccgacaaggc caagaatggc atcaaggtga
     1801 acttcaagat ccgccacaac atcgaggatg gcagcgtgca gctggccgac cactaccagc
     1861 agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc
     1921 agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac ttcggcttcg
     1981 tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtgagcg gccgcgactc
     2041 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2101 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2281 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2341 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2401 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2461 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2521 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2581 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2641 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2701 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     2761 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2821 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     2881 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2941 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3001 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3061 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3121 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3181 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3241 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3301 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3361 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3421 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3481 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3541 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3601 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3661 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3721 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3781 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3841 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3901 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3961 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4021 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4081 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4141 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4201 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4261 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4321 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4381 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4441 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4501 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4561 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4621 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4681 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4741 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4801 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4861 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4921 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4981 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5041 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5101 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5161 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5221 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5281 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5341 aaccgtatta ccgccatgca t
//
