LOCUS       pEThT-6xHis-CAHS8        6797 bp    DNA     circular UNA 06-JUN-2020
DEFINITION  His6(N)-MCS-GST(C) was inserted to NdeI-XhoI sites of pET-22b(+)
            http://wolfson.huji.ac.il/expression/commercial-vectors/pet-22b-map
            .pdf / generated by T.Kunieda 2000.12.19 T7=> NdeI - His6 - NcoI,
            BamHI, SmaI, EcoRI, HindIII, ClaI, NheI, SpeI, AvrII - Thrombin/TEV
            sites - GST.
ACCESSION   urn.local...3r-c0bp3wb
VERSION     urn.local...3r-c0bp3wb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             4..1433
                     /created_by="User"
                     /label="His6-GST"
     primer          15..50
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS8-pET-Fw"
     misc_feature    30..699
                     /Original_Bases="CCCCGGGCTGCAGG"
                     /label="CCCCGGGCTGCAGG"
                     /note="Geneious type: Editing History Replacement"
     CDS             31..699
                     /created_by="saibou"
                     /label="CAHS8"
     primer          complement(675..714)
                     /created_by="saibou"
                     /label="CAHS8-pET-Rv"
     primer          complement(804..823)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="RvSHAS1 seq Rv"
     primer          6742..6761
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="RvSAHS1 seq fw"
ORIGIN      
        1 catatgcacc atcaccatca ccatggatca atgtctcagc gagcttcgga acactcatca
       61 tccagccacc agtcgtctgg ccagtctgga cagtcgagcc agcacgtcca agagcagaac
      121 accagctaca cacacaccga tgtccgcacc aacgttccca atgtccctcc tccagtcatc
      181 tcgaccagcg ctgctggtct cagtgagcag atggtcggtc aaggtttcac cgcctcggct
      241 gcgcgagtct ccggccagtc caccgaagcc catgtccaaa tgacccctga gatggaagcc
      301 gaggcccgca aggaccgcga acgatacgag cgcgaactgg aagccatcaa ccaacgccac
      361 cagaaggata ttgaaggcaa gaccgaggct taccgaaagc aagccgaagc tgaggcggaa
      421 cgtcttcgac gggaactaga gaagcaacat caacgagacg ttgagttccg caagtccctg
      481 gttcaaggga caatcgacaa ccagaagaaa caggttgagc tggaggctat gatggccaag
      541 cgcgagttgg accgagaagc caagctggct caagatgctc tcgagaagag caaaatggcc
      601 accgatgctc aagtcacgtt cgagtcagca gtaggccaca cgatttctgg tagcaccacc
      661 gtgtcccagt ccgagaaagt atcccagtcc aagaagtaaa attcgatatc aagcttatcg
      721 atgctagcac tagtcctagg ctggttccgc gtgggtccga gaacctgtac ttccagggct
      781 cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga cttcttttgg
      841 aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt gataaatggc
      901 gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat attgatggtg
      961 atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag cacaacatgt
     1021 tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg gttttggata
     1081 ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc aaagttgatt
     1141 ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat
     1201 atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct cttgatgttg
     1261 ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt tttaaaaaac
     1321 gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat atagcatggc
     1381 ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa taactcgagc
     1441 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     1501 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     1561 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     1621 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1681 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1741 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1801 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1861 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1921 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1981 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2041 caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2161 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2221 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2281 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2341 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2401 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2461 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2521 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2581 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2641 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2701 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     2761 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2821 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2881 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2941 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3001 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3061 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3121 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3181 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3241 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3301 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3361 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3421 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3481 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3541 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3601 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3661 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3721 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3781 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3841 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3901 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3961 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4021 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
     4081 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     4141 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4201 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4261 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     4321 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     4381 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     4441 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     4501 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     4561 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     4621 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     4681 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     4741 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     4801 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     4861 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga
     4921 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     4981 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     5041 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     5101 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     5161 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     5221 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     5341 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     5401 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     5461 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     5521 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     5581 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     5641 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     5701 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     5761 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     5821 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     5881 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     5941 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     6001 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     6061 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     6121 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     6181 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     6241 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     6301 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     6361 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     6421 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     6481 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     6541 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     6601 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     6661 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
     6721 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
     6781 tttaagaagg agatata
//
