LOCUS       pAc5.1/V5-His_A_CAHS3_DM_for_GA_GS_AcGFP        7032 bp    DNA     circular SYN 22-SEP-2022
DEFINITION  pAc5.1/V5-His A.
ACCESSION   urn.local...1p-fc36hf3
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="pAc5.1/V5-His A"
                     /mol_type="other DNA"
     CDS             726..1454
                     /translation="MMPDHRLISASFAERSACAVVVFWNSFNFRTVRFDFSFLAYSKS
                     KVKPKKRDGNTKCGKTVVEGKGCGAAEGRVGRGVAGSVAGRDVTDVGATPLTMCACVY
                     YSCLATRRLFFSFYLALSSAISYACSTHRMLPCPLCVILARNRQLFKQRLVNENAKIN
                     KSTIWLLIAMCVQPTIATKATTQWLSLFHFLFASACEQDGTTGKRNYADKEQTKFWPK
                     NIKAPDTNAFAITIAIFNIVYEAV*"
                     /label="ORF frame 3"
     CDS             complement(1940..2416)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="ORF frame 2"
     promoter        2510..2571
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="promotor"
     misc_feature    2525..2544
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="AC5_primer"
     misc_feature    2608..4287
                     /Original_Bases="CTGCAGATATCCAGCACAGTGGC"
                     /label="CTGCAGATATCCAGCACAGTGGC"
                     /note="Geneious type: Editing History Replacement"
     motif           2608..2613
                     /created_by="saibou"
                     /label="kozak_DM"
     CDS             2614..>3522
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS3_DM_for_GeneArt"
     primer          complement(3503..3542)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="CAHS3_DM_iF"
     linker          3523..3567
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="pBS Coupler segment"
     CDS             3568..4287
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="AcGFP"
     primer          complement(3575..3596)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="seq-AcGFP1-N1-Rev"
     primer          complement(3762..3783)
                     /created_by="saibou"
                     /modified_by="saibou"
                     /label="seq_AcGFP"
     misc_feature    complement(4408..4425)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="BGH_rev_primer"
     terminator      complement(4468..4587)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="SV40_PA_terminator"
     misc_feature    complement(4482..4501)
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
                     /label="EBV_rev_primer"
     CDS             complement(4615..5244)
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAGKSRERSLKLSLMVVIYL
                     PGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQRVGRHAGDNGLLLAETFGGG
                     TSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCRHLSYELHDKEDSHKCGDD
                     SHAPRPPEGADWVEGSQGHRSTDHNQPYHICRGFTCFKKPPTPPPEPET*"
                     /label="ORF frame 1"
     CDS             4693..5295
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="ORF frame 3"
     rep_origin      complement(5199..5818)
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="pBR322_origin"
     gene            complement(5973..6833)
                     /gene="Ampicillin"
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
                     /label="Ampicillin"
     CDS             complement(5973..6833)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 2"
     promoter        complement(6875..6903)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaattgcc accatgagta gccgccagaa tcagcagagc
     2641 agcagccagc atagtagcag ctcccagcaa ggcggacaag gtggacaggg tgttcaaggc
     2701 agcagcagct atagccgcac cgaggttcac acaagcagcg gcggaccaac aattggaggt
     2761 gcccaacgta cagtgccagt gccaccagga tcgcatagcg aggtgcacga agaacgcgaa
     2821 gtgatcaagc acggcaccaa gaccgagagc gagacacacg ttgtgaccgt gccagtgaca
     2881 accttcggca gcaccaacat ggaaagcgtg cgcaccggat tcaccgtgac gcaggataag
     2941 aacctgaccg tggccgctcc gaatatcgcc gctccaatcc acagcaacct ggatctgaat
     3001 ctcggaggcg gagcccgtgc cgaaattacc gctggaacaa cggtggatct gagcaagatc
     3061 cagcgcaagg atctgggccc cgaagagtac gcccgttaca aggccaaggt ggaacagctg
     3121 gcccgccagg atgaacagga tgctggaatg cgtgccgcgc agtaccgtga agaggtggaa
     3181 cgtgatgccg agctgatccg ccagattctg gaacgccagc acatccgcga tttggagttc
     3241 cgcaaagaga tggtcgagaa tcaagtgaac cgccaagagc gcgagatcca gttggaggcc
     3301 gaatatgcca tgcgcgccct ggaactggaa cgcaatgccg ccaaagaagc cctggaaagc
     3361 gccaaggctc agaccaacgt gaacgtgaag gtcgagagcg ccatcggaac cacagtgtcc
     3421 aagggcgcca ttcagaccag cgccgataag agcagcacca ccaagacagg acccaccacc
     3481 gtgacacaga ttaagcacac cgagcagcat accgagcgcc gcggtggagg cggttcaggc
     3541 ggaggtggct ctggcggtgg cggatcgatg gtgagcaagg gcgccgagct gttcaccggc
     3601 atcgtgccca tcctgatcga gctgaatggc gatgtgaatg gccacaagtt cagcgtgagc
     3661 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3721 ggcaagctgc ctgtgccctg gcccaccctg gtgaccaccc tgagctacgg cgtgcagtgc
     3781 ttctcacgct accccgatca catgaagcag cacgacttct tcaagagcgc catgcctgag
     3841 ggctacatcc aggagcgcac catcttcttc gaggatgacg gcaactacaa gtcgcgcgcc
     3901 gaggtgaagt tcgagggcga taccctggtg aatcgcatcg agctgaccgg caccgatttc
     3961 aaggaggatg gcaacatcct gggcaataag atggagtaca actacaacgc ccacaatgtg
     4021 tacatcatga ccgacaaggc caagaatggc atcaaggtga acttcaagat ccgccacaac
     4081 atcgaggatg gcagcgtgca gctggccgac cactaccagc agaatacccc catcggcgat
     4141 ggccctgtgc tgctgcccga taaccactac ctgtccaccc agagcgccct gtccaaggac
     4201 cccaacgaga agcgcgatca catgatctac ttcggcttcg tgaccgccgc cgccatcacc
     4261 cacggcatgg atgagctgta caagtgaggc cgctcgagtc tagagggccc ttcgaaggta
     4321 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     4381 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
     4441 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     4501 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4561 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4621 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4681 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     4741 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     4801 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     4861 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     4921 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     4981 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     5041 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     5101 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     5161 cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5221 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5281 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5341 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5401 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5461 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5521 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5581 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5641 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5701 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5761 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5821 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5881 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5941 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6001 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6061 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6121 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6181 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6241 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     6301 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6361 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6421 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6481 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6541 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     6601 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6661 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6721 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6781 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6961 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7021 aggccctttc gt
//
